FastQCFastQC Report
Thu 26 May 2016
SRR805286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15417130
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1534640.9954122459887151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT827950.042.5267871
TCAACGC842850.041.72201512
ATCAACG844050.041.7183811
GTGGTAT842050.041.613746
CAACGCA844850.041.57874313
AGTGGTA847950.041.318655
GTATCAA849300.041.3138479
GGTATCA851300.041.2581948
AACGCAG854600.041.10987514
ACGCAGA855950.041.03954715
TATCAAC856750.040.95185510
CGCAGAG860450.040.81126416
TGGTATC862350.040.677747
AGAGTAC857150.040.3953819
CAGTGGT887000.039.5155034
CAGAGTA881750.039.4229718
GCAGTGG893000.039.4210553
AGCAGTG905100.038.9667432
GTACATG612000.038.84795822
GAGTACT277550.038.6600620
Produced by FastQC (version 0.11.5)