##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805286_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15417130
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.65243128909207	37.0	35.0	37.0	28.0	37.0
2	35.77155384951674	37.0	35.0	37.0	33.0	37.0
3	37.911994709780615	39.0	38.0	39.0	35.0	39.0
4	38.0903992507036	39.0	39.0	39.0	37.0	39.0
5	38.09192119415222	39.0	39.0	39.0	37.0	39.0
6	38.113835584184606	39.0	39.0	39.0	37.0	39.0
7	38.06148414134148	39.0	39.0	39.0	37.0	39.0
8	39.0208235903829	40.0	40.0	40.0	37.0	40.0
9	39.071551773903444	40.0	40.0	40.0	37.0	40.0
10	38.88787783459048	40.0	40.0	40.0	36.0	40.0
11	39.0504915636049	40.0	40.0	40.0	37.0	40.0
12	39.10885210152603	40.0	40.0	40.0	37.0	40.0
13	39.15523810203326	40.0	40.0	40.0	38.0	40.0
14	39.11628409438073	40.0	40.0	40.0	37.0	40.0
15	39.09239054220857	40.0	40.0	40.0	37.0	40.0
16	39.06488561749171	40.0	40.0	40.0	37.0	40.0
17	38.9993225068479	40.0	40.0	40.0	37.0	40.0
18	38.962878823749946	40.0	40.0	40.0	37.0	40.0
19	38.93669820517827	40.0	40.0	40.0	37.0	40.0
20	38.89192774530668	40.0	40.0	40.0	37.0	40.0
21	38.84640487561563	40.0	40.0	40.0	37.0	40.0
22	38.835044006245	40.0	40.0	40.0	37.0	40.0
23	38.65448303283426	40.0	39.0	40.0	36.0	40.0
24	38.76779958396926	40.0	39.0	40.0	36.0	40.0
25	38.80238786337016	40.0	39.0	40.0	37.0	40.0
26	38.820004177171754	40.0	40.0	40.0	37.0	40.0
27	38.824180635436036	40.0	40.0	40.0	37.0	40.0
28	38.80279448898725	40.0	40.0	40.0	37.0	40.0
29	38.748355562935515	40.0	40.0	40.0	36.0	40.0
30	38.69050367999751	40.0	39.0	40.0	36.0	40.0
31	38.67328108409282	40.0	39.0	40.0	36.0	40.0
32	38.666409831142374	40.0	39.0	40.0	36.0	40.0
33	38.227495325005364	40.0	39.0	40.0	35.0	40.0
34	38.43850554545496	40.0	39.0	40.0	35.0	40.0
35	38.51721182866072	40.0	39.0	40.0	35.0	40.0
36	38.490079022489915	40.0	39.0	40.0	35.0	40.0
37	38.43388847340588	40.0	39.0	40.0	35.0	40.0
38	38.29140216110262	40.0	38.0	40.0	35.0	40.0
39	38.2900403641923	40.0	38.0	40.0	35.0	40.0
40	38.27670351096475	40.0	38.0	40.0	35.0	40.0
41	38.252112228410866	40.0	38.0	40.0	35.0	40.0
42	38.14936152189156	40.0	38.0	40.0	35.0	40.0
43	38.08692071740979	40.0	38.0	40.0	35.0	40.0
44	37.9889176519884	40.0	38.0	40.0	34.0	40.0
45	37.47085676776417	40.0	38.0	40.0	33.0	40.0
46	37.688220116195424	40.0	38.0	40.0	33.0	40.0
47	37.74083652404825	40.0	38.0	40.0	34.0	40.0
48	37.65333301334295	40.0	37.0	40.0	34.0	40.0
49	37.656586148005495	40.0	38.0	40.0	34.0	40.0
50	37.613039391897196	40.0	37.0	40.0	33.0	40.0
51	37.62249841572329	40.0	37.0	40.0	34.0	40.0
52	37.52713793034112	40.0	37.0	40.0	33.0	40.0
53	37.10777083672512	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	12.0
11	38.0
12	64.0
13	164.0
14	323.0
15	673.0
16	1326.0
17	2105.0
18	3310.0
19	4603.0
20	6531.0
21	8901.0
22	12573.0
23	17376.0
24	23695.0
25	32705.0
26	44607.0
27	58534.0
28	75761.0
29	95575.0
30	119658.0
31	148082.0
32	181668.0
33	223404.0
34	285414.0
35	389443.0
36	565330.0
37	997352.0
38	2198103.0
39	9919797.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.699077584479083	36.76847117459605	29.475745485703243	22.056705755221625
2	28.864386562220073	41.6642332262879	13.925004199873777	15.546376011618246
3	31.183151468528834	27.986428083566782	20.18026701467783	20.65015343322655
4	22.170397473459715	26.574453221838308	21.354837119489815	29.900312185212165
5	20.697406067147387	31.61520334848315	25.299183440757133	22.38820714361233
6	26.78813761056695	25.572437931054615	23.940752915750206	23.698671542628233
7	22.86356150593528	25.09011729161005	29.441361654211907	22.604959548242768
8	26.126503441301978	26.755239139839905	25.380359379469457	21.737898039388654
9	26.576042363267355	26.111792532073093	25.5608339554768	21.75133114918276
10	21.69574363062386	26.682073771188282	29.832543411127755	21.789639187060107
11	21.49859279904885	31.255292003116015	25.58328949681296	21.662825701022175
12	21.287185098653254	26.18941398301759	30.328472290238196	22.194928628090963
13	21.44116965998211	26.52188831514037	25.578068032117525	26.45887399276
14	21.900892059676476	31.25670601467329	25.205424096443373	21.636977829206863
15	21.546014076549916	31.004953580854544	25.216444305781945	22.232588036813596
16	22.04589959350411	25.98811192485242	25.393202236732776	26.572786244910695
17	26.81375846217811	25.854487832690005	25.316508325479514	22.015245379652374
18	21.785779843589566	26.16575847774521	25.33403428523986	26.714427393425368
19	22.1419615713171	30.830083160743925	25.20052694632529	21.827428321613684
20	26.964344206736275	26.241609171097345	25.220238786337017	21.573807835829367
21	22.130630019984267	30.65543976083746	25.273504212522045	21.94042600665623
22	26.562369260686005	25.80532174276276	25.702546453198487	21.92976254335275
23	21.949578164029234	26.090601817588617	30.236918285050464	21.72290173333169
24	21.848619036098157	30.489916086846254	25.371505591507628	22.28995928554796
25	21.771477570728145	26.05247539587459	25.51829685551072	26.657750177886548
26	22.080095322540576	29.17792092302523	26.99404493573058	21.74793881870361
27	22.18137227875746	25.787659570879924	30.095225246203412	21.935742904159206
28	25.340514090495443	25.857127753349683	26.875306882668827	21.92705127348605
29	25.539416220788176	25.856725603273762	26.77206458011316	21.8317935958249
30	25.4402602819072	25.801352132335914	26.748298807884474	22.010088777872404
31	23.762049097335236	26.59091542978492	27.105537801134194	22.54149767174565
32	23.36944035627902	26.959330303370344	27.415186873302616	22.25604246704802
33	23.394315284362264	26.561779008155213	27.448026967405738	22.595878740076785
34	23.035863354593236	26.66404836697881	27.617539710698424	22.682548567729533
35	22.975761377117532	26.64876666409377	27.646072907214247	22.72939905157445
36	22.87563249450449	26.665209413165748	27.650042517641094	22.809115574688672
37	22.973653332364712	26.855387481327586	27.654161312773518	22.516797873534177
38	23.149373456668005	26.74657994062449	27.63542241649386	22.468624186213646
39	23.005585345651234	26.539933178224484	27.738301486722882	22.7161799894014
40	22.81502458628811	26.7585276896543	27.67936055543412	22.747087168623473
41	22.943530994419845	26.79269747352458	27.666686341751028	22.59708519030455
42	23.067912121127605	26.657315596352888	27.637478570914304	22.637293711605206
43	22.964929270233824	26.669911974537413	27.767866003594705	22.59729275163406
44	22.919959810937574	26.682994824587976	27.713497907846662	22.68354745662779
45	22.987903714893758	26.50904545787705	27.606895706269587	22.89615512095961
46	22.891763901582202	26.605217702646343	27.65474507901276	22.848273316758696
47	23.101991096916223	26.697173857910002	27.565934775149458	22.634900270024318
48	22.98853288517383	26.58625827245408	27.608407012200065	22.81680183017202
49	22.847754413434927	26.72390386537572	27.592950179443253	22.835391541746098
50	23.078685851387384	26.667006115924302	27.532835229384457	22.721472803303858
51	23.01139706287746	26.581211937630417	27.514537400930006	22.89285359856212
52	22.99400082894806	26.6434997953575	27.49669361288385	22.865805762810588
53	23.28129165415353	26.697478713612714	27.246050334919662	22.77517929731409
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	473.0
1	403.0
2	333.0
3	318.0
4	303.0
5	337.0
6	371.0
7	465.5
8	769.0
9	978.0
10	1527.5
11	2077.0
12	3246.5
13	4416.0
14	6634.5
15	8853.0
16	13908.0
17	18963.0
18	28664.5
19	38366.0
20	52522.5
21	66679.0
22	173488.5
23	280298.0
24	228796.0
25	193240.0
26	209186.0
27	234701.0
28	260216.0
29	294715.5
30	329215.0
31	367425.5
32	405636.0
33	447701.0
34	489766.0
35	540962.0
36	592158.0
37	644957.0
38	697756.0
39	757993.0
40	818230.0
41	868551.5
42	971911.5
43	1024950.0
44	1057038.5
45	1089127.0
46	1109153.0
47	1129179.0
48	1135153.0
49	1141127.0
50	1127395.5
51	1113664.0
52	1075900.0
53	1038136.0
54	980720.5
55	923305.0
56	858526.0
57	793747.0
58	703534.5
59	530441.5
60	447561.0
61	379927.5
62	312294.0
63	258963.0
64	205632.0
65	165071.5
66	124511.0
67	97250.5
68	69990.0
69	53037.0
70	36084.0
71	26797.0
72	17510.0
73	12881.5
74	8253.0
75	6101.0
76	2795.5
77	1642.0
78	1243.5
79	845.0
80	684.0
81	523.0
82	418.0
83	313.0
84	237.0
85	161.0
86	137.5
87	114.0
88	81.5
89	49.0
90	46.0
91	43.0
92	39.5
93	32.0
94	28.0
95	25.0
96	22.0
97	17.0
98	12.0
99	6.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.541713E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.56105479475537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.70629038932108	42.25466584223297
2	13.94508448132136	15.774973749310202
3	4.899322846981166	8.313326040158959
4	2.2101679456978185	5.00037721128905
5	1.1564374836634501	3.270466194009871
6	0.7065586975649476	2.3978223125209195
7	0.4696796123701335	1.859590200387261
8	0.33862408896857243	1.5322348520780438
9	0.23683644188283123	1.2056147070056742
>10	1.2408873648252992	12.41451139905651
>50	0.06794730698820611	2.622968362923738
>100	0.02171222568566163	1.9103548964171446
>500	2.4469267945004453E-4	0.0883524112215585
>1k	1.605538725792412E-4	0.23566138654991545
>5k	3.440113305392761E-5	0.12373115639416907
>10k+	1.1467044351309203E-5	0.9953492784439253
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	153464	0.9954122459887151	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9836941116796707E-4	0.0	0.0	6.486291547129719E-6	6.486291547129719E-6
2	0.0039566378437491286	0.0	0.0	1.2972583094259438E-5	6.486291547129719E-6
3	0.0039825830099376475	0.0	0.0	1.2972583094259438E-5	6.486291547129719E-6
4	0.004125281423974501	0.0	0.0	1.2972583094259438E-5	6.486291547129719E-6
5	0.004125281423974501	0.0	0.0	1.2972583094259438E-5	1.2972583094259438E-5
6	0.004138254007068761	6.486291547129719E-6	0.0	1.2972583094259438E-5	1.2972583094259438E-5
7	0.00414474029861589	6.486291547129719E-6	0.0	1.2972583094259438E-5	1.2972583094259438E-5
8	0.00415122659016302	1.2972583094259438E-5	0.0	1.2972583094259438E-5	1.2972583094259438E-5
9	0.00415122659016302	1.9458874641389157E-5	0.0	1.2972583094259438E-5	1.2972583094259438E-5
10	0.004183658047898668	2.5945166188518875E-5	0.0	1.2972583094259438E-5	1.2972583094259438E-5
11	0.004196630630992928	2.5945166188518875E-5	0.0	2.5945166188518875E-5	1.2972583094259438E-5
12	0.004255007254917095	2.5945166188518875E-5	0.0	2.5945166188518875E-5	1.2972583094259438E-5
13	0.004501486333708025	2.5945166188518875E-5	0.0	4.540404082990803E-5	1.9458874641389157E-5
14	0.004553376666085063	2.5945166188518875E-5	0.0	4.540404082990803E-5	1.9458874641389157E-5
15	0.004760937995593214	2.5945166188518875E-5	0.0	4.540404082990803E-5	1.9458874641389157E-5
16	0.0047868831617817326	2.5945166188518875E-5	0.0	5.189033237703775E-5	1.9458874641389157E-5
17	0.004793369453328862	2.5945166188518875E-5	0.0	7.134920701842691E-5	1.9458874641389157E-5
18	0.004929581575818586	2.5945166188518875E-5	0.0	7.134920701842691E-5	1.9458874641389157E-5
19	0.004962013033554234	3.2431457735648594E-5	0.0	7.134920701842691E-5	2.5945166188518875E-5
20	0.0050203896574784026	3.2431457735648594E-5	0.0	7.134920701842691E-5	2.5945166188518875E-5
21	0.005098225156043959	3.2431457735648594E-5	0.0	7.783549856555663E-5	2.5945166188518875E-5
22	0.005176060654609516	3.2431457735648594E-5	0.0	8.432179011268635E-5	2.5945166188518875E-5
23	0.005234437278533683	3.2431457735648594E-5	0.0	8.432179011268635E-5	2.5945166188518875E-5
24	0.005403080858759055	3.891774928277831E-5	0.0	8.432179011268635E-5	2.5945166188518875E-5
25	0.0054614574826832235	3.891774928277831E-5	0.0	8.432179011268635E-5	3.2431457735648594E-5
26	0.005584697022078687	3.891774928277831E-5	0.0	8.432179011268635E-5	3.891774928277831E-5
27	0.0058506349755110066	3.891774928277831E-5	0.0	9.080808165981606E-5	3.891774928277831E-5
28	0.005909011599435174	4.540404082990803E-5	0.0	9.080808165981606E-5	3.891774928277831E-5
29	0.006058196305019158	4.540404082990803E-5	0.0	1.037806647540755E-4	3.891774928277831E-5
30	0.006187922135961752	4.540404082990803E-5	0.0	1.037806647540755E-4	3.891774928277831E-5
31	0.006479805255582589	4.540404082990803E-5	0.0	1.1026695630120522E-4	3.891774928277831E-5
32	0.006635476252713702	4.540404082990803E-5	0.0	1.1026695630120522E-4	3.891774928277831E-5
33	0.006771688375203426	4.540404082990803E-5	0.0	1.1675324784833494E-4	3.891774928277831E-5
34	0.006894927914598891	4.540404082990803E-5	0.0	1.2323953939546466E-4	3.891774928277831E-5
35	0.007011681162447226	4.540404082990803E-5	0.0	1.2323953939546466E-4	4.540404082990803E-5
36	0.00714789328493695	4.540404082990803E-5	0.0	1.2972583094259438E-4	4.540404082990803E-5
37	0.007420317529916398	4.540404082990803E-5	0.0	1.2972583094259438E-4	4.540404082990803E-5
38	0.00760193369323603	4.540404082990803E-5	0.0	1.2972583094259438E-4	4.540404082990803E-5
39	0.00782895389738557	4.540404082990803E-5	0.0	1.4269841403685381E-4	4.540404082990803E-5
40	0.007984624894516684	4.540404082990803E-5	0.0	1.4918470558398353E-4	4.540404082990803E-5
41	0.008211645098666225	4.540404082990803E-5	0.0	1.5567099713111325E-4	4.540404082990803E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	82795	0.0	42.526787	1
TCAACGC	84285	0.0	41.722015	12
ATCAACG	84405	0.0	41.71838	11
GTGGTAT	84205	0.0	41.61374	6
CAACGCA	84485	0.0	41.578743	13
AGTGGTA	84795	0.0	41.31865	5
GTATCAA	84930	0.0	41.313847	9
GGTATCA	85130	0.0	41.258194	8
AACGCAG	85460	0.0	41.109875	14
ACGCAGA	85595	0.0	41.039547	15
TATCAAC	85675	0.0	40.951855	10
CGCAGAG	86045	0.0	40.811264	16
TGGTATC	86235	0.0	40.67774	7
AGAGTAC	85715	0.0	40.39538	19
CAGTGGT	88700	0.0	39.515503	4
CAGAGTA	88175	0.0	39.42297	18
GCAGTGG	89300	0.0	39.421055	3
AGCAGTG	90510	0.0	38.966743	2
GTACATG	61200	0.0	38.847958	22
GAGTACT	27755	0.0	38.66006	20
>>END_MODULE