FastQCFastQC Report
Thu 26 May 2016
SRR805285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41221354
Sequences flagged as poor quality0
Sequence length73
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG279200.020.49117511
AAGCAGT277100.020.2610821
TCAACGC287900.019.93439912
GGTATCA283350.019.104928
GTATCAA281600.019.0956179
GAGTACT115750.019.02170220
AGTGGTA291100.018.3608765
CAACGCA313600.018.3015913
AACGCAG313650.018.19674914
GTGGTAT294900.017.9346946
CGCAGAG314650.017.65896416
ACGCAGA317050.017.56010215
TATCAAC309350.017.51713410
AGAGTAC294150.017.49079719
TGGTATC308950.017.1407347
CAGAGTA329850.015.74767518
CAGTGGT358400.015.1319354
GTACTTT146700.014.7744322
AGCGGTT120550.014.67969710
AGTACTT153200.014.30379421
Produced by FastQC (version 0.11.5)