##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR805285_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41221354 Sequences flagged as poor quality 0 Sequence length 73 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.006646555084046 35.0 25.0 39.0 4.0 40.0 2 28.351967186715896 35.0 19.0 39.0 4.0 40.0 3 28.204120272225897 35.0 17.0 39.0 4.0 40.0 4 28.049146832973996 35.0 15.0 39.0 4.0 40.0 5 27.88119334944699 35.0 14.0 39.0 4.0 40.0 6 27.583916894141808 35.0 10.0 39.0 4.0 40.0 7 27.395542077535833 35.0 8.0 39.0 4.0 40.0 8 27.235743420752264 35.0 4.0 39.0 4.0 40.0 9 27.07291601823657 35.0 4.0 39.0 4.0 40.0 10 26.948986804266546 35.0 4.0 39.0 4.0 40.0 11 26.97790984740579 35.0 4.0 39.0 4.0 40.0 12 26.73004644631518 35.0 4.0 39.0 4.0 40.0 13 26.766791891406577 35.0 4.0 39.0 4.0 40.0 14 26.672449308676274 35.0 4.0 39.0 4.0 40.0 15 26.566814520454617 35.0 4.0 39.0 4.0 40.0 16 26.470627723679335 35.0 4.0 39.0 4.0 40.0 17 26.31668309585367 35.0 4.0 39.0 4.0 40.0 18 26.19785422380837 34.0 4.0 39.0 4.0 40.0 19 26.094167576348898 34.0 4.0 39.0 4.0 40.0 20 25.955650340840332 34.0 4.0 39.0 4.0 40.0 21 25.726642336881994 33.0 4.0 39.0 4.0 40.0 22 25.566728133190384 33.0 4.0 38.0 4.0 40.0 23 25.447081966303195 33.0 4.0 38.0 4.0 40.0 24 25.181321773176105 33.0 4.0 38.0 4.0 40.0 25 25.196422635704785 33.0 4.0 38.0 4.0 40.0 26 23.678206664439017 31.0 4.0 38.0 4.0 40.0 27 23.375261472488265 31.0 4.0 38.0 4.0 40.0 28 23.148606593563134 31.0 4.0 38.0 4.0 40.0 29 22.91543041017042 30.0 4.0 38.0 4.0 40.0 30 22.664854798316426 30.0 4.0 38.0 4.0 40.0 31 20.922688953885405 25.0 4.0 37.0 4.0 39.0 32 20.1921050191607 21.0 4.0 37.0 4.0 39.0 33 19.870348970099332 19.0 4.0 36.0 4.0 39.0 34 19.396518100788246 17.0 4.0 36.0 4.0 39.0 35 18.794870081172007 10.0 4.0 36.0 4.0 39.0 36 18.29925948089915 4.0 4.0 36.0 4.0 39.0 37 18.14177775431637 4.0 4.0 35.0 4.0 39.0 38 18.089320937880885 4.0 4.0 35.0 4.0 39.0 39 17.86589935886143 4.0 4.0 35.0 4.0 39.0 40 17.59312663528714 4.0 4.0 35.0 4.0 39.0 41 17.53196214758011 4.0 4.0 35.0 4.0 39.0 42 17.362572636502914 4.0 4.0 35.0 4.0 39.0 43 17.322454546252896 4.0 4.0 35.0 4.0 39.0 44 17.17801334230797 4.0 4.0 35.0 4.0 39.0 45 17.107901234879378 4.0 4.0 35.0 4.0 39.0 46 17.277410853607574 4.0 4.0 35.0 4.0 39.0 47 17.14922976571803 4.0 4.0 35.0 4.0 39.0 48 16.963217244149718 4.0 4.0 35.0 4.0 39.0 49 16.92665476248063 4.0 4.0 35.0 4.0 39.0 50 16.80906289492577 4.0 4.0 34.0 4.0 39.0 51 16.660338352786763 4.0 4.0 34.0 4.0 39.0 52 16.565724381591153 4.0 4.0 34.0 4.0 39.0 53 16.416442482699622 4.0 4.0 33.0 4.0 38.0 54 16.27982392329956 4.0 4.0 33.0 4.0 38.0 55 16.128417082078382 4.0 4.0 33.0 4.0 38.0 56 15.483146186804053 4.0 4.0 33.0 4.0 38.0 57 15.194922442382655 4.0 4.0 33.0 4.0 38.0 58 15.092896002397204 4.0 4.0 32.0 4.0 38.0 59 15.141315032980236 4.0 4.0 32.0 4.0 38.0 60 14.778447039852209 4.0 4.0 31.0 4.0 38.0 61 14.809224825560072 4.0 4.0 32.0 4.0 38.0 62 14.733922107459158 4.0 4.0 32.0 4.0 38.0 63 14.6381053373453 4.0 4.0 31.0 4.0 38.0 64 14.499882924757882 4.0 4.0 31.0 4.0 38.0 65 14.381930515916581 4.0 4.0 31.0 4.0 38.0 66 14.378205868734929 4.0 4.0 31.0 4.0 38.0 67 14.156358037147445 4.0 4.0 31.0 4.0 37.0 68 13.960306786623264 4.0 4.0 31.0 4.0 37.0 69 13.634333724214882 4.0 4.0 30.0 4.0 36.0 70 13.457413431882902 4.0 4.0 30.0 4.0 36.0 71 13.312253498514387 4.0 4.0 30.0 4.0 36.0 72 13.059696219585605 4.0 4.0 29.0 4.0 36.0 73 12.791181701600584 4.0 4.0 28.0 4.0 36.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 0.25230722670577777 2 2 0.4907724486359797 2 3 0.6225790935178104 2 4 0.3874774618675012 2 5 0.26248066499319833 2 6 0.5880260131527955 2 7 0.539055566768738 2 8 0.524054682260136 2 9 0.3838978940812936 2 10 0.4399147048918479 2 11 0.6003557578612906 2 12 0.48715830495766 2 13 0.6654964934825358 2 14 0.5057336907208736 2 15 0.6472090832292388 2 16 0.6241827408171687 2 17 0.6116479848227563 2 18 0.6058602502848487 2 19 0.5252792509039708 2 20 0.30624977728909286 2 21 0.6408650014718944 2 22 0.824219368369203 2 23 0.7145810539371595 2 24 0.5063124560721057 2 25 0.7882902543245827 2 26 1.8887348142333948 2 27 2.062419880323475 2 28 2.3925698338617494 2 29 1.9961443611969898 2 30 2.4986446515472736 2 31 4.31156052897941 2 32 4.971568530930476 2 33 5.218787285346998 2 34 5.580509741082704 2 35 5.946545617528308 2 36 6.563418135888796 2 37 6.54081176716376 2 38 6.356800540189376 2 39 6.222381338988939 2 40 5.6119547149914375 2 41 -2.145163686049324 2 42 -4.803918670446624 2 43 -6.451060065505352 2 44 -7.8511880013569115 2 45 -8.059872999059412 2 46 -10.190969860619017 2 47 -10.012018214413686 2 48 -10.007510276437543 2 49 -9.884103716422644 2 50 -10.0029804840484 2 51 -9.529401056162847 2 52 -9.461980002232483 2 53 -9.703785588889716 2 54 -9.217996836248123 2 55 -9.593790983263897 2 56 -5.258345960322703 2 57 -4.4526731082952065 2 58 -4.3166833932242845 2 59 -4.405203551984629 2 60 -4.133261077635257 2 61 -3.126850468490831 2 62 -3.1632318045294117 2 63 -3.781798511171141 2 64 -7.648879888420579 2 65 -7.616015362857557 2 66 -9.951471925934497 2 67 -9.785428468693038 2 68 -9.547404971935357 2 69 -9.23207737576172 2 70 -9.103571999653465 2 71 -8.863344409143089 2 72 -8.602498624548675 2 73 -8.350514357874351 3 1 0.25927438245532386 3 2 0.6486246942582135 3 3 0.8326525186472473 3 4 0.8741821588725713 3 5 0.8415730911801873 3 6 0.720213028240245 3 7 0.7957134763510858 3 8 0.8427390444133138 3 9 0.8060474137255511 3 10 0.6534672726452726 3 11 0.774905061106935 3 12 0.5850509241261541 3 13 0.6468776513139431 3 14 0.7244614740877893 3 15 0.6508827076136932 3 16 0.5924827955456671 3 17 0.8183416283249407 3 18 0.5331947394260474 3 19 0.8994534567296277 3 20 0.8815884541095613 3 21 0.7068882883913723 3 22 0.8755860039266032 3 23 1.0192170974224553 3 24 0.6709544691227265 3 25 1.059139455685603 3 26 2.3034593467550053 3 27 2.588047796208901 3 28 2.668692207405538 3 29 2.8528025230672114 3 30 2.845407960939834 3 31 4.19621435164791 3 32 5.141110068089056 3 33 5.4406464703081845 3 34 5.946874150405485 3 35 6.1949754792345 3 36 6.74813520869964 3 37 6.777539564979207 3 38 6.769848642990173 3 39 6.84414852571674 3 40 7.025740580903875 3 41 6.724576528933632 3 42 7.030675738427352 3 43 6.413658332378304 3 44 6.799715122981372 3 45 7.081358284801766 3 46 6.90144788932275 3 47 6.5957411394867975 3 48 6.765539323620672 3 49 6.654370612705904 3 50 6.733295374101882 3 51 6.455059850127814 3 52 6.66561588858945 3 53 6.588187926273552 3 54 6.634717469481284 3 55 6.577967315369559 3 56 7.155528402486651 3 57 7.377235831128864 3 58 7.647456867155519 3 59 7.245026838874795 3 60 7.22965628053039 3 61 6.9992424985429675 3 62 7.041058150898358 3 63 6.55160750278038 3 64 6.04764090232748 3 65 5.785749465689811 3 66 2.632503802324962 3 67 2.0448093508090626 3 68 0.9671894953316578 3 69 -4.496105292425721 3 70 -6.394067270322608 3 71 -7.973024643993904 3 72 -8.197817592969756 3 73 -8.032323380220282 4 1 0.18411551901575152 4 2 0.5863841478516498 4 3 0.753606162414691 4 4 0.8040486354489111 4 5 0.8075464198299933 4 6 0.9582434015400665 4 7 0.8505868671505503 4 8 0.8448394253172857 4 9 0.9319039552060531 4 10 1.0414812020901358 4 11 1.0916814244295914 4 12 1.2504147146733935 4 13 1.1751840686917205 4 14 1.0047363745434374 4 15 1.1587430688243323 4 16 1.2164450078235163 4 17 1.273433032973358 4 18 1.2379261396454169 4 19 1.3663166490766372 4 20 1.5115660147144183 4 21 1.553479312568662 4 22 1.5743744059838036 4 23 1.6022193934454698 4 24 1.7312540145324604 4 25 1.786821218641279 4 26 3.024415838860264 4 27 3.2491050645877806 4 28 3.417673340301505 4 29 3.5805627368211184 4 30 3.6696778794453913 4 31 5.500349278657431 4 32 6.067359648550148 4 33 6.325786454637328 4 34 6.784997683426397 4 35 7.142093914287827 4 36 7.687179644596778 4 37 7.633456495141768 4 38 7.705687887260673 4 39 7.790649047536398 4 40 8.125049722114731 4 41 7.96368363766242 4 42 8.053099126571173 4 43 8.073237397340208 4 44 7.864656289800344 4 45 8.04208866125186 4 46 7.774580612050311 4 47 7.87591265472528 4 48 7.8777627697982275 4 49 7.702506719382136 4 50 7.871281820319396 4 51 7.816570901775979 4 52 7.879563366198436 4 53 8.04984689088483 4 54 8.126421501037292 4 55 8.097019677138075 4 56 8.88658158270752 4 57 8.839797888939408 4 58 9.073704094253145 4 59 8.826602237784746 4 60 9.130048831872978 4 61 8.915862663641699 4 62 8.747683461919184 4 63 8.617041385730802 4 64 8.372620921033658 4 65 8.246727896736527 4 66 8.560729506557628 4 67 8.094434369680387 4 68 8.164843760754104 4 69 8.41732069932675 4 70 8.038651119393911 4 71 8.52929238046643 4 72 8.104443323087185 4 73 8.05588001298044 5 1 0.09089684541550724 5 2 0.35539187477172973 5 3 0.5555310738135866 5 4 0.5779381232884688 5 5 0.6795566062586396 5 6 0.7193134845554852 5 7 0.5204397445909663 5 8 0.7813264167142506 5 9 0.689724069164761 5 10 0.7537761847711977 5 11 0.7289447014845507 5 12 0.9730741189959033 5 13 0.805126919678699 5 14 0.8376225536016797 5 15 0.6912241050918126 5 16 0.9722182712707941 5 17 0.8381828512652589 5 18 0.9389057932632348 5 19 0.8413688851795555 5 20 1.0003190690848989 5 21 1.0578178430406489 5 22 1.0600908768863313 5 23 1.1117301113311697 5 24 1.086566071982773 5 25 1.2559998753708896 5 26 2.473320394726059 5 27 2.8175748885160203 5 28 3.030154549572046 5 29 3.198335142444108 5 30 3.2065907400249607 5 31 5.136585403217669 5 32 5.7458156168795504 5 33 5.746334772434789 5 34 6.460490424326814 5 35 6.859932008607746 5 36 7.413447110267924 5 37 7.4163299009334445 5 38 7.371725584270937 5 39 7.441823326627649 5 40 7.428654899128912 5 41 7.60675580515359 5 42 7.596386795425492 5 43 7.641762316301591 5 44 7.557268286454676 5 45 7.667442127718921 5 46 7.516701238612317 5 47 7.351380776896189 5 48 7.535326017282657 5 49 7.609494468413615 5 50 7.582107832468967 5 51 7.594401236859472 5 52 7.531166162214127 5 53 7.537981415412009 5 54 7.5254934815440215 5 55 7.9141987756479715 5 56 8.308534922462476 5 57 8.50084979515471 5 58 8.702044440477579 5 59 8.646532855315376 5 60 8.628665290845953 5 61 8.636537569739213 5 62 8.633132089944421 5 63 8.496965298714999 5 64 8.144064311588561 5 65 8.133275164283088 5 66 8.119324866661394 5 67 7.946440513664866 5 68 8.059985784904496 5 69 8.182773246546118 5 70 7.8808123054502985 5 71 7.705635151812221 5 72 7.468584036411997 5 73 7.54039252173977 6 1 0.08541769722059556 6 2 0.39206075544851515 6 3 0.615933663566345 6 4 0.6725682170368401 6 5 0.620246861474083 6 6 0.4793099198160924 6 7 0.67127098335245 6 8 0.7079212422970755 6 9 0.775702691415102 6 10 0.7018450486890622 6 11 0.7687585371494698 6 12 0.9035497141263669 6 13 0.5925299229053138 6 14 0.5216401632902112 6 15 0.45774608547281304 6 16 0.49379201425716346 6 17 0.5408362493068601 6 18 0.6335078267729948 6 19 0.41840337359284874 6 20 0.7256803678627364 6 21 0.6751748221699962 6 22 1.0122546933695808 6 23 0.8264933621416795 6 24 0.7476957927236789 6 25 0.746851957634064 6 26 2.340743423802966 6 27 2.4467325619551126 6 28 2.52683133657127 6 29 2.6827272354146245 6 30 2.803374310058885 6 31 4.854995355742357 6 32 5.276948022888909 6 33 5.7113658564529075 6 34 5.941302671398059 6 35 6.272956467660563 6 36 7.321768447998242 6 37 7.3814135837729005 6 38 7.169192213701649 6 39 7.411007271092796 6 40 7.370965852555493 6 41 7.398226352882794 6 42 7.459898166230879 6 43 7.623592147033566 6 44 7.371358028721371 6 45 7.54384554531099 6 46 7.351639059805205 6 47 7.233355572408286 6 48 7.370361674200478 6 49 6.97834044099897 6 50 7.041587155279899 6 51 6.991309693418469 6 52 7.343257379963141 6 53 7.139433346140326 6 54 6.940601239397637 6 55 7.262946247519746 6 56 8.112571362044276 6 57 8.406350777990216 6 58 8.421313635420184 6 59 8.24309768925899 6 60 8.411427373395098 6 61 7.6910054374965515 6 62 8.223977463556793 6 63 7.97556973112818 6 64 7.894901362259102 6 65 8.037871073138675 6 66 7.585960277469514 6 67 7.539409855101061 6 68 7.449121369566415 6 69 7.49585602639017 6 70 7.894163897700924 6 71 7.66467206046493 6 72 7.4725741786810005 6 73 7.037624061116892 7 1 0.4196924891851026 7 2 0.711635587878888 7 3 0.9208844511320677 7 4 0.8353743075326072 7 5 0.8740113813464774 7 6 0.9518019818876979 7 7 1.0081319882213542 7 8 0.9769023887268169 7 9 0.9253467922107852 7 10 0.9524127271109855 7 11 0.7417756135640765 7 12 0.7082283253251305 7 13 0.9736691079010953 7 14 0.9692210146668998 7 15 0.788319989625748 7 16 0.9529882954750555 7 17 1.0499490867981862 7 18 0.8261152444700706 7 19 0.8039999320076348 7 20 0.9351670109895558 7 21 1.020950903262328 7 22 0.9683438126495041 7 23 1.070762940108473 7 24 1.1450113553557415 7 25 0.8939300333839029 7 26 2.5919236810793223 7 27 2.8350538311721074 7 28 2.6809702046982835 7 29 2.6612257618309734 7 30 3.2994718990512837 7 31 5.0149512742659965 7 32 5.640889605449903 7 33 6.037551202733884 7 34 6.339056117263549 7 35 6.77024818278878 7 36 7.258236841058224 7 37 7.478792974363429 7 38 7.372948599487966 7 39 7.463606862245751 7 40 7.83279154817501 7 41 7.660671885971066 7 42 7.567697334952246 7 43 7.357670037599458 7 44 7.600077572749331 7 45 7.598273884272711 7 46 7.527798807638828 7 47 7.552725891169803 7 48 7.712171079457672 7 49 7.3732016835801275 7 50 7.523782582210721 7 51 7.189287107495424 7 52 7.282170722925855 7 53 7.393550221723444 7 54 7.1747675872827905 7 55 7.385464254760194 7 56 7.805109912186078 7 57 8.282755429078662 7 58 8.403955414256743 7 59 8.144175422725377 7 60 8.235290171126136 7 61 7.799230302191093 7 62 8.048017149922241 7 63 7.434709800125061 7 64 7.719822145934263 7 65 7.816494570114031 7 66 7.684939857064855 7 67 7.549942907141565 7 68 7.445216514172332 7 69 7.861346873237638 7 70 7.772933449368892 7 71 7.801347520797513 7 72 7.779123109594771 7 73 7.512809715737289 8 1 0.4726909060306532 8 2 0.7841774657335421 8 3 0.8757002324923242 8 4 0.9803341391461835 8 5 1.0402878725088485 8 6 1.0944686058241082 8 7 1.039976387957232 8 8 0.90351031763878 8 9 0.9307849888974111 8 10 1.0552599630518245 8 11 1.0757741627194548 8 12 1.0877176589076392 8 13 1.0334839214416576 8 14 1.0760780201858573 8 15 1.1039075859431335 8 16 1.0544785330437811 8 17 0.906690530542317 8 18 0.9936593044329385 8 19 0.9557974397304498 8 20 1.1318886907197836 8 21 1.342617558063889 8 22 1.2434361702040775 8 23 1.2266985359597307 8 24 1.2530282191089732 8 25 1.4130053441054535 8 26 2.824832587241957 8 27 3.0671503806966776 8 28 3.022381333671607 8 29 3.351085083648929 8 30 3.429547561368885 8 31 5.195002337717263 8 32 5.809654592042055 8 33 6.094051745127992 8 34 6.526249282923956 8 35 7.105855947565445 8 36 7.472852267670042 8 37 7.619113630183357 8 38 7.214625961615845 8 39 7.563216658963615 8 40 7.433453969546157 8 41 7.890481136562272 8 42 7.4810652928099906 8 43 7.3181468979071305 8 44 7.589116733149297 8 45 7.562835450839504 8 46 7.609855203499233 8 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1452132.0 19 1606062.0 20 1117253.0 21 626643.0 22 580416.0 23 558519.0 24 545157.0 25 542143.0 26 563972.0 27 613847.0 28 683473.0 29 748348.0 30 788938.0 31 790008.0 32 855414.0 33 983791.0 34 1163329.0 35 1410470.0 36 1710111.0 37 1985928.0 38 2103530.0 39 1100853.0 40 2.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.274895166292808 30.703492622871213 23.768977368137445 27.25263484269853 2 24.288045033171002 31.050149352484546 24.169283762312315 20.492521852032137 3 23.831788952002025 28.84492303476087 24.656899168635814 22.666388844601286 4 26.35429178511543 27.603519415547527 23.30740690374227 22.734781895594775 5 26.391434173565422 29.382265511648903 24.123515692400392 20.10278462238528 6 25.986211751720816 27.785395712330786 21.661670701270495 24.566721834677903 7 24.13033620686141 27.134707455875752 25.753734277899 22.981222059363844 8 24.48596937430067 26.629328139795476 25.24961377085414 23.635088715049708 9 23.79868315222083 26.487823410823918 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28.925101592575786 48 23.221962061456832 24.035734874366288 23.54467243096882 29.19763063320806 49 23.318425084784565 24.16177505050911 23.385968005610227 29.133831859096098 50 23.43136015439688 24.125785635068546 23.49406443637139 28.948789774163185 51 23.268863517412875 23.88465560886254 23.45481753520458 29.391663338520008 52 23.30689306242915 23.984892210131356 23.32793260355335 29.380282123886143 53 23.12390696937685 24.039870564217463 23.689723751228968 29.146498715176715 54 23.199304545733284 24.10704503453651 23.363583467517486 29.330066952212714 55 23.07102833489853 24.154024926070907 23.533628031124206 29.241318707906355 56 22.14927108526892 24.9235244225518 23.916420836317016 29.01078365586226 57 21.544796069427928 25.5375082283936 24.609130562465793 28.308565139712684 58 22.514417516706597 25.03178735557944 22.610273083292686 29.843522044421277 59 22.631083619140398 24.775544695686232 23.430298095966748 29.16307358920662 60 22.07644322224803 24.66409647117577 23.840921639725945 29.418538666850257 61 22.428832339723307 24.893794270025644 23.675753223555798 29.001620166695254 62 22.19315444887765 24.82721961217422 23.74805727303881 29.231568665909318 63 22.60748846391093 24.90269645330436 23.46328567288092 29.026529409903784 64 23.112984304546778 24.229897695025386 23.218358616413585 29.43875938401425 65 22.72714547379379 23.948257964808327 23.714671241017744 29.609925320380135 66 22.84927005403906 23.91786556618267 23.51297139831866 29.719892981459612 67 23.030052592878477 23.619212362889687 23.15899957832972 30.191735465902113 68 22.923805379802527 23.590387403450254 23.129756444492646 30.356050772254573 69 22.995841947455705 23.318908024459958 23.06633880183988 30.618911226244457 70 22.898281110687122 23.509350806659484 23.11416774219953 30.478200340453864 71 22.79418546581631 23.545029433770857 23.09169337486592 30.569091725546915 72 22.77441101974282 23.418845750783028 23.04333377852376 30.76340945095039 73 22.507389823595915 23.082746696932357 23.0230076401743 31.38685583929743 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 516.0 1 464.0 2 634.0 3 1473.0 4 2090.0 5 3750.5 6 8869.0 7 12327.0 8 18790.0 9 35601.5 10 61442.0 11 76934.0 12 97197.5 13 142085.5 14 194286.0 15 221862.0 16 250122.0 17 303111.5 18 346724.5 19 365608.0 20 377855.5 21 395032.0 22 399961.0 23 399765.5 24 395813.5 25 389508.0 26 386959.0 27 390388.5 28 404781.5 29 433322.0 30 450899.0 31 482536.0 32 559032.0 33 603891.0 34 664381.5 35 783075.0 36 908357.5 37 975437.0 38 1050860.0 39 1208456.0 40 1383324.5 41 1476020.0 42 1562224.5 43 1715129.0 44 1781829.0 45 1837727.0 46 1929090.0 47 1989837.0 48 2015119.0 49 2029527.5 50 2052949.5 51 2048012.5 52 2034062.0 53 1996102.5 54 1900034.0 55 1754368.5 56 1666812.0 57 1569183.5 58 1361065.0 59 1250575.0 60 1146897.5 61 951843.5 62 783341.0 63 706215.0 64 638956.0 65 516952.0 66 415148.0 67 368089.0 68 330243.5 69 260264.5 70 228131.0 71 202285.0 72 154668.0 73 115826.5 74 98756.0 75 85311.5 76 61425.5 77 42879.0 78 34774.0 79 28708.5 80 18639.0 81 14635.0 82 11845.5 83 7319.5 84 4469.5 85 3356.0 86 2779.0 87 1780.5 88 1129.0 89 899.0 90 721.5 91 443.5 92 284.5 93 226.0 94 187.5 95 126.5 96 104.0 97 92.5 98 79.5 99 98.5 100 119.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.8836827630649882 2 1.107147038401504 3 2.132355962882733 4 3.044681647284075 5 3.6012305660799013 6 3.94149110191771 7 4.187720277213602 8 4.32694665973369 9 4.366894401382352 10 4.361372991289903 11 4.363684899821583 12 4.441021030022449 13 4.505502172490501 14 4.534065038232368 15 4.562009292562297 16 4.633392197645909 17 4.694139838298373 18 4.720706166032295 19 4.736770655325878 20 4.841197113515485 21 4.852824582132843 22 4.879781484130773 23 4.968650471791877 24 5.328767220989393 25 5.3295556472987275 26 7.393696965897821 27 7.6979470397794305 28 8.27612552464919 29 7.957021499099715 30 8.335267201557716 31 9.278972253070581 32 9.104395260767028 33 11.289204619528025 34 9.190328876630302 35 12.923391599412284 36 10.694051922700066 37 11.398174839186504 38 11.4371061173779 39 11.548730301289957 40 12.269298577625568 41 9.570372191073588 42 7.552767432142088 43 6.386983309670032 44 7.095065339192885 45 6.97867178259113 46 6.4682639973446765 47 6.591399205373021 48 6.768309939552204 49 6.619852904395135 50 6.455365342923962 51 6.723520047400674 52 6.731215088179781 53 6.698472350034887 54 7.667084395141412 55 7.273516537084153 56 11.067722326636819 57 13.741896978929901 58 10.533157159272353 59 9.102837815565204 60 10.357013018058552 61 10.026829783417595 62 11.54048942691208 63 8.900158883669858 64 7.099334970898822 65 7.280905911047948 66 7.228670848609194 67 6.966013294953872 68 6.948301601155557 69 7.335773104396329 70 7.531506606988213 71 7.10128541629176 72 7.309281980402682 73 7.493317662491145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 73 4.1221354E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.88766237619421 #Duplication Level Percentage of deduplicated Percentage of total 1 99.89492069652042 99.78270111630728 2 0.09973287199570217 0.19924166891429787 3 0.00401424076481936 0.012029193786390956 4 0.0010047809483281192 0.004014608805145257 5 2.0120053868686165E-4 0.001004872573913082 6 0.0 0.0 7 0.0 0.0 8 1.2620923207476672E-4 0.0010085396129794434 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.911112526774351E-5 2.4259271056452925E-6 0.0 9.70370842258117E-6 2.4259271056452925E-6 2 3.638890658467939E-5 4.851854211290585E-6 0.0 1.6981489739517046E-5 2.4259271056452925E-6 3 5.3370396324196435E-5 4.851854211290585E-6 0.0 2.1833343950807633E-5 4.851854211290585E-6 4 9.70370842258117E-5 4.851854211290585E-6 0.0 2.4259271056452924E-5 9.70370842258117E-6 5 3.469075761072768E-4 7.277781316935878E-6 0.0 2.4259271056452924E-5 9.70370842258117E-6 6 3.5903721163550327E-4 7.277781316935878E-6 0.0 2.6685198162098218E-5 9.70370842258117E-6 7 3.7116684716372977E-4 9.70370842258117E-6 0.0 2.911112526774351E-5 9.70370842258117E-6 8 4.4151873322744324E-4 1.2129635528226462E-5 0.0 3.638890658467939E-5 9.70370842258117E-6 9 7.302040587992331E-4 1.2129635528226462E-5 0.0 3.881483369032468E-5 9.70370842258117E-6 10 0.0016908711926347689 1.2129635528226462E-5 0.0 4.366668790161527E-5 9.70370842258117E-6 11 0.001940741684516234 1.6981489739517046E-5 0.0 4.851854211290585E-5 1.2129635528226462E-5 12 0.0021275380716509213 1.940741684516234E-5 0.0 5.3370396324196435E-5 1.6981489739517046E-5 13 0.0022585381353557674 2.1833343950807633E-5 0.0 6.064817764113231E-5 1.6981489739517046E-5 14 0.002421075251434002 2.1833343950807633E-5 0.0 6.55000318524229E-5 1.6981489739517046E-5 15 0.0039324278382510194 2.1833343950807633E-5 0.0 7.277781316935878E-5 1.940741684516234E-5 16 0.004116798298280062 2.1833343950807633E-5 0.0 8.490744869758524E-5 2.1833343950807633E-5 17 0.004427316967802659 2.4259271056452924E-5 0.0 9.461115712016641E-5 2.4259271056452924E-5 18 0.004672335605472833 2.911112526774351E-5 0.0 1.1159264685968345E-4 2.4259271056452924E-5 19 0.005077465432115597 3.15370523733888E-5 0.0 1.1644450107097404E-4 2.6685198162098218E-5 20 0.0367430919421036 3.15370523733888E-5 0.0 1.1887042817661933E-4 2.911112526774351E-5 21 0.03754607381407219 3.638890658467939E-5 0.0 1.261482094935552E-4 2.911112526774351E-5 22 0.037929370296764145 3.881483369032468E-5 0.0 1.285741365992005E-4 3.15370523733888E-5 23 0.03821562969523029 3.881483369032468E-5 0.0 1.3827784502178168E-4 3.15370523733888E-5 24 0.03850431502080208 4.366668790161527E-5 0.0 1.4555562633871756E-4 3.396297947903409E-5 25 0.03879785220058516 4.609261500726056E-5 0.0 1.5525933476129872E-4 3.396297947903409E-5 26 0.039047722692466626 5.3370396324196435E-5 0.0 1.6496304318387988E-4 3.638890658467939E-5 27 0.039346111726461 5.5796323429841725E-5 0.0 1.6981489739517047E-4 3.881483369032468E-5 28 0.04189090926028291 5.5796323429841725E-5 0.0 1.7709267871210635E-4 3.881483369032468E-5 29 0.04234455762903858 5.822225053548702E-5 0.0 1.7709267871210635E-4 3.881483369032468E-5 30 0.04353568783791042 5.822225053548702E-5 0.0 1.8437046002904223E-4 4.124076079596997E-5 31 0.07081523814089173 5.822225053548702E-5 0.0 1.8922231424033282E-4 4.124076079596997E-5 32 0.07137320137519015 5.822225053548702E-5 0.0 1.916482413459781E-4 4.366668790161527E-5 33 0.07198210907870711 5.822225053548702E-5 2.4259271056452925E-6 1.940741684516234E-4 5.0944469218551145E-5 34 0.07236297963429343 5.822225053548702E-5 4.851854211290585E-6 1.940741684516234E-4 5.3370396324196435E-5 35 0.07274627611698539 5.822225053548702E-5 4.851854211290585E-6 1.940741684516234E-4 5.3370396324196435E-5 36 0.07324359117364267 5.822225053548702E-5 4.851854211290585E-6 1.940741684516234E-4 5.3370396324196435E-5 37 0.07378457291820158 5.822225053548702E-5 4.851854211290585E-6 1.965000955572687E-4 5.3370396324196435E-5 38 0.07442259174698629 6.064817764113231E-5 4.851854211290585E-6 1.9892602266291398E-4 5.5796323429841725E-5 39 0.0751624995142081 6.064817764113231E-5 4.851854211290585E-6 2.0620380397984986E-4 5.822225053548702E-5 40 0.0760091480740783 6.30741047467776E-5 4.851854211290585E-6 2.1105565819114046E-4 5.822225053548702E-5 41 0.07681698180025819 6.55000318524229E-5 4.851854211290585E-6 2.1348158529678574E-4 6.792595895806818E-5 42 0.07779948227804453 6.55000318524229E-5 4.851854211290585E-6 2.1590751240243102E-4 7.277781316935878E-5 43 0.078692223452922 6.792595895806818E-5 9.70370842258117E-6 2.2075936661372162E-4 7.277781316935878E-5 44 0.07979359435888496 7.035188606371348E-5 1.2129635528226462E-5 2.231852937193669E-4 7.520374027500406E-5 45 0.08102353940144712 7.520374027500406E-5 1.2129635528226462E-5 2.231852937193669E-4 8.005559448629466E-5 46 0.08222437331874155 7.520374027500406E-5 1.2129635528226462E-5 2.256112208250122E-4 8.248152159193994E-5 47 0.08338154054813435 7.520374027500406E-5 1.4555562633871756E-5 2.3531492924759337E-4 8.733337580323053E-5 48 0.08468426340386587 7.520374027500406E-5 1.4555562633871756E-5 2.498704918814651E-4 9.218523001452111E-5 49 0.08606218999987239 7.520374027500406E-5 1.4555562633871756E-5 2.6685198162098217E-4 9.218523001452111E-5 50 0.0876317648372249 8.005559448629466E-5 1.4555562633871756E-5 2.7170383583227274E-4 9.218523001452111E-5 51 0.0894148212598742 8.005559448629466E-5 1.4555562633871756E-5 2.7170383583227274E-4 9.70370842258117E-5 52 0.09133615552754526 8.005559448629466E-5 1.6981489739517046E-5 2.814075442548539E-4 1.0674079264839287E-4 53 0.09328417499337843 8.005559448629466E-5 1.6981489739517046E-5 2.886853255717898E-4 1.0674079264839287E-4 54 0.09520793518815515 8.248152159193994E-5 1.940741684516234E-5 2.959631068887257E-4 1.0916671975403817E-4 55 0.09720932505031252 8.490744869758524E-5 1.940741684516234E-5 3.0566681531130687E-4 1.1159264685968345E-4 56 0.09959158546805619 8.490744869758524E-5 1.940741684516234E-5 3.202223779451786E-4 1.1644450107097404E-4 57 0.1023450127329636 8.733337580323053E-5 1.940741684516234E-5 3.275001592621145E-4 1.1644450107097404E-4 58 0.1054138105216049 8.975930290887582E-5 1.940741684516234E-5 3.3235201347340507E-4 1.1644450107097404E-4 59 0.10861360837395104 9.218523001452111E-5 1.940741684516234E-5 3.347779405790504E-4 1.1644450107097404E-4 60 0.11224764717820768 9.218523001452111E-5 2.4259271056452924E-5 3.3720386768469564E-4 1.285741365992005E-4 61 0.1162067602146208 9.70370842258117E-5 2.4259271056452924E-5 3.3962979479034095E-4 1.285741365992005E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCAACG 27920 0.0 20.491175 11 AAGCAGT 27710 0.0 20.261082 1 TCAACGC 28790 0.0 19.934399 12 GGTATCA 28335 0.0 19.10492 8 GTATCAA 28160 0.0 19.095617 9 GAGTACT 11575 0.0 19.021702 20 AGTGGTA 29110 0.0 18.360876 5 CAACGCA 31360 0.0 18.30159 13 AACGCAG 31365 0.0 18.196749 14 GTGGTAT 29490 0.0 17.934694 6 CGCAGAG 31465 0.0 17.658964 16 ACGCAGA 31705 0.0 17.560102 15 TATCAAC 30935 0.0 17.517134 10 AGAGTAC 29415 0.0 17.490797 19 TGGTATC 30895 0.0 17.140734 7 CAGAGTA 32985 0.0 15.747675 18 CAGTGGT 35840 0.0 15.131935 4 GTACTTT 14670 0.0 14.77443 22 AGCGGTT 12055 0.0 14.679697 10 AGTACTT 15320 0.0 14.303794 21 >>END_MODULE