FastQCFastQC Report
Thu 26 May 2016
SRR805284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41380713
Sequences flagged as poor quality0
Sequence length73
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT279000.022.646281
ATCAACG308700.020.73810811
TCAACGC320700.020.08966412
GTATCAA305000.019.7641689
GGTATCA306700.019.667898
AGTGGTA306050.019.2414995
CAACGCA337300.018.69774813
AACGCAG339150.018.60059414
GTGGTAT325750.018.1596476
GAGTACT124750.017.96114520
CGCAGAG338000.017.9225816
ACGCAGA342100.017.84365315
TGGTATC330600.017.8414737
TATCAAC346950.017.60152410
AGAGTAC325450.017.17824719
CAGTGGT383900.015.6454774
CAGAGTA362500.015.4632518
GTACTTT145100.015.047976522
AGCAGTG443650.014.72779752
GCAGTGG443100.014.7263973
Produced by FastQC (version 0.11.5)