##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805284_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41380713
Sequences flagged as poor quality	0
Sequence length	73
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.636587097955513	36.0	28.0	39.0	4.0	40.0
2	29.200662033058734	36.0	23.0	39.0	4.0	40.0
3	29.151658841644416	37.0	23.0	39.0	4.0	40.0
4	28.952776889078734	36.0	22.0	39.0	4.0	40.0
5	28.815219302770352	36.0	21.0	39.0	4.0	40.0
6	28.47929739634984	36.0	18.0	39.0	4.0	40.0
7	28.304626384760457	36.0	17.0	39.0	4.0	40.0
8	28.161297220760794	36.0	16.0	39.0	4.0	40.0
9	27.974565832154706	35.0	14.0	39.0	4.0	40.0
10	27.837693589281557	35.0	13.0	39.0	4.0	40.0
11	27.81285593121607	35.0	10.0	39.0	4.0	40.0
12	27.70809806974568	35.0	9.0	39.0	4.0	40.0
13	27.591224853955513	35.0	7.0	39.0	4.0	40.0
14	27.502465822664774	35.0	4.0	39.0	4.0	40.0
15	27.386669219546796	35.0	4.0	39.0	4.0	40.0
16	27.267958577707446	35.0	4.0	39.0	4.0	40.0
17	27.103944753199396	35.0	4.0	39.0	4.0	40.0
18	26.983115467343445	35.0	4.0	39.0	4.0	40.0
19	26.830808159347086	35.0	4.0	39.0	4.0	40.0
20	26.713992506605674	35.0	4.0	39.0	4.0	40.0
21	26.530739767582062	35.0	4.0	39.0	4.0	40.0
22	26.344703195423435	35.0	4.0	39.0	4.0	40.0
23	26.22054194667936	34.0	4.0	39.0	4.0	40.0
24	26.200010110990597	35.0	4.0	39.0	4.0	40.0
25	26.110378692604932	35.0	4.0	39.0	4.0	40.0
26	24.869349931210706	33.0	4.0	38.0	4.0	40.0
27	24.613228897240123	33.0	4.0	38.0	4.0	40.0
28	24.067600285186	32.0	4.0	38.0	4.0	40.0
29	24.1344930668546	32.0	4.0	38.0	4.0	40.0
30	23.1720529803341	31.0	4.0	38.0	4.0	40.0
31	21.081474647379807	26.0	4.0	37.0	4.0	39.0
32	19.834411504702686	18.0	4.0	36.0	4.0	39.0
33	19.180867569874884	15.0	4.0	36.0	4.0	39.0
34	19.358597784431602	17.0	4.0	36.0	4.0	39.0
35	19.254462531856326	15.0	4.0	36.0	4.0	39.0
36	18.734335244537714	7.0	4.0	36.0	4.0	39.0
37	18.558140334604673	4.0	4.0	36.0	4.0	39.0
38	18.414524346160977	4.0	4.0	36.0	4.0	39.0
39	18.294752606123534	4.0	4.0	36.0	4.0	39.0
40	18.130859272531143	4.0	4.0	35.0	4.0	39.0
41	17.692400442689326	4.0	4.0	35.0	4.0	39.0
42	17.488437330695582	4.0	4.0	35.0	4.0	39.0
43	17.34348424590944	4.0	4.0	35.0	4.0	39.0
44	17.271670210225714	4.0	4.0	35.0	4.0	39.0
45	17.090856288532294	4.0	4.0	35.0	4.0	39.0
46	17.19349207443574	4.0	4.0	35.0	4.0	39.0
47	16.998544249346306	4.0	4.0	35.0	4.0	39.0
48	16.86970630496386	4.0	4.0	34.0	4.0	39.0
49	16.76129219909768	4.0	4.0	34.0	4.0	39.0
50	16.670913016892676	4.0	4.0	34.0	4.0	39.0
51	16.509877294767733	4.0	4.0	34.0	4.0	39.0
52	16.353822782125576	4.0	4.0	33.0	4.0	38.0
53	16.276858061870513	4.0	4.0	33.0	4.0	38.0
54	16.116800234930704	4.0	4.0	33.0	4.0	38.0
55	15.96979720963242	4.0	4.0	33.0	4.0	38.0
56	15.553854835705707	4.0	4.0	33.0	4.0	38.0
57	15.536533602018892	4.0	4.0	33.0	4.0	38.0
58	15.465827932930976	4.0	4.0	33.0	4.0	38.0
59	15.322905649305753	4.0	4.0	33.0	4.0	38.0
60	15.10075162310519	4.0	4.0	33.0	4.0	38.0
61	14.868131851667226	4.0	4.0	33.0	4.0	38.0
62	14.691266556958553	4.0	4.0	32.0	4.0	38.0
63	14.504212940941834	4.0	4.0	32.0	4.0	38.0
64	14.344648677271461	4.0	4.0	31.0	4.0	38.0
65	14.1660791828309	4.0	4.0	31.0	4.0	37.0
66	13.866896976859726	4.0	4.0	31.0	4.0	37.0
67	13.680629524194037	4.0	4.0	30.0	4.0	37.0
68	13.574096197907465	4.0	4.0	30.0	4.0	36.0
69	13.37281820156168	4.0	4.0	29.0	4.0	36.0
70	13.183746012302882	4.0	4.0	29.0	4.0	36.0
71	12.963233330464847	4.0	4.0	29.0	4.0	36.0
72	12.832779295030514	4.0	4.0	29.0	4.0	36.0
73	12.697517343405853	4.0	4.0	28.0	4.0	36.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2	1	0.10522301772294185
2	2	0.26540052395703384
2	3	0.36574485572529625
2	4	0.5191062916008811
2	5	0.5299964455415349
2	6	0.5023950489286513
2	7	0.5776146701884777
2	8	0.5501542181441152
2	9	0.47608917074159507
2	10	0.5602627815163643
2	11	0.7201107442379104
2	12	0.6235841352685547
2	13	0.6716487562285955
2	14	0.7102450102531037
2	15	0.6502025649293337
2	16	0.8222810075919931
2	17	0.8254842686359716
2	18	0.856195591607932
2	19	0.8084724337691895
2	20	0.6492051318857115
2	21	1.0206951405565512
2	22	1.0991354852590085
2	23	1.0167743173303414
2	24	1.0024253115561876
2	25	0.9427080700352377
2	26	2.083751427735521
2	27	2.085831397083126
2	28	2.365795571337408
2	29	2.2585814681667777
2	30	3.2208673811201827
2	31	5.186546564692392
2	32	6.206820491193479
2	33	6.767968305561684
2	34	6.682063252317587
2	35	6.561436515929049
2	36	7.170541053619807
2	37	7.0351783093363345
2	38	7.050316655449237
2	39	7.1048479623039
2	40	7.190122755752899
2	41	7.31317823158356
2	42	7.216867703305887
2	43	7.349699670231605
2	44	7.1780549794994
2	45	6.798340427919989
2	46	1.0602844517681334
2	47	-5.992382447851142
2	48	-3.898960958519929
2	49	-6.054641748036376
2	50	-5.9011076777310105
2	51	-5.390655348328604
2	52	-4.438185232085452
2	53	-1.8693557936286993
2	54	-0.7457752232912789
2	55	-0.6464204866703476
2	56	5.942508103643647
2	57	5.860318110534337
2	58	5.855117969435568
2	59	5.802095701890391
2	60	5.924822638173307
2	61	7.163255647498914
2	62	7.095026380016559
2	63	7.034585132798728
2	64	7.01145631425986
2	65	6.607157635727432
2	66	3.185461046465017
2	67	2.782647667832695
2	68	1.5307151441076563
2	69	-3.5438499484405206
2	70	-3.8747357648315806
2	71	-7.18751894151556
2	72	-6.268692343191502
2	73	-6.58811255432068
3	1	-0.24100150181801894
3	2	-0.14673829028113872
3	3	-0.0966005922165607
3	4	0.05454012688290888
3	5	0.058096475350268406
3	6	0.0500924909832996
3	7	0.0482754458576089
3	8	0.055609859526896344
3	9	0.012856352129631432
3	10	-0.014730150977975143
3	11	0.01018079213618961
3	12	0.14830452854921816
3	13	0.31616367806917367
3	14	0.1266171702006318
3	15	0.3107488452854277
3	16	0.1440964259129629
3	17	0.207284590252943
3	18	-0.13868308880303104
3	19	0.19835200065872982
3	20	0.3149444000128909
3	21	0.38956382250697175
3	22	0.32313239826294193
3	23	0.41073968756293766
3	24	0.3006364374498425
3	25	0.4380044498722633
3	26	1.453366760394747
3	27	1.7912010354500083
3	28	2.0406090811661706
3	29	1.9691058136974924
3	30	2.922496175680699
3	31	4.832398752752777
3	32	5.603846675503043
3	33	6.46338979675112
3	34	5.759497626895911
3	35	6.2093128911963476
3	36	6.60531717044249
3	37	6.539945757453765
3	38	6.8296449254644
3	39	6.731783079181742
3	40	6.933730671928021
3	41	7.241481784001461
3	42	7.255061657933602
3	43	7.299845044486094
3	44	7.15510150678973
3	45	7.407564223684307
3	46	7.3159147926706005
3	47	7.149564118408325
3	48	7.465270806570807
3	49	7.279376266076483
3	50	7.394445841514106
3	51	7.402829992818216
3	52	7.262050618365574
3	53	6.885368177747434
3	54	6.96814731756513
3	55	7.267332625640314
3	56	7.194807853596068
3	57	7.530453010196862
3	58	7.450733956045813
3	59	7.658799986332291
3	60	7.6758054229763495
3	61	7.846657025017024
3	62	7.530898498689886
3	63	7.740837129059425
3	64	7.601519243387152
3	65	7.331436552946798
3	66	7.587481024676128
3	67	7.60787166027626
3	68	7.408977870613409
3	69	7.63565488385936
3	70	7.26328755333445
3	71	7.451813146831142
3	72	7.298868475637798
3	73	6.958831118254174
4	1	-0.26521505283484004
4	2	-0.11110953447208871
4	3	-0.09800578349955202
4	4	0.004869638356087336
4	5	0.10099798344297284
4	6	0.021887319508717695
4	7	0.0012263483082115556
4	8	0.05836586395746224
4	9	-0.07321552792685182
4	10	0.1072162733335702
4	11	0.04280273156905068
4	12	0.09183296878901359
4	13	0.1075239572688993
4	14	-0.010507418613219244
4	15	0.14985492742987816
4	16	0.00403507592850616
4	17	-0.019815674809041894
4	18	0.008553892472491498
4	19	0.13714001495591788
4	20	0.042605133947184015
4	21	0.055588120568046406
4	22	-0.05947150452219674
4	23	0.31317808217565
4	24	0.038143682913979404
4	25	0.4942447940245529
4	26	1.5618816763479977
4	27	1.5585808745871148
4	28	1.838626925296321
4	29	1.8379007528609144
4	30	2.522002471402395
4	31	4.522038710079563
4	32	5.354991264234783
4	33	6.33465207139108
4	34	6.314479423297378
4	35	6.2671265225189785
4	36	6.730628610401443
4	37	6.419963597124632
4	38	6.443922240195988
4	39	6.607153178822529
4	40	6.728310447139467
4	41	6.958581138661916
4	42	7.1526739790361695
4	43	7.1450672351447615
4	44	6.979117257773865
4	45	6.835625000024741
4	46	6.9554629092005555
4	47	6.921508685038486
4	48	6.818993141280327
4	49	6.689561018109689
4	50	7.0084379134363886
4	51	7.011092215093374
4	52	7.10486879293763
4	53	7.0361053296510185
4	54	6.680502171212829
4	55	7.14129312765834
4	56	7.403868291354744
4	57	6.972752797122123
4	58	7.086572645431616
4	59	7.137270510371216
4	60	6.955503185702632
4	61	6.771039655746598
4	62	7.265187175394715
4	63	7.3165867475162365
4	64	6.6847323452598495
4	65	7.085416101727741
4	66	7.026846606803664
4	67	6.806279516754962
4	68	6.6596349302956455
4	69	6.828944800138814
4	70	6.971817708597921
4	71	6.84809398943597
4	72	6.074859872816804
4	73	6.569526689860314
5	1	-0.23313231885169827
5	2	-0.18747153119466375
5	3	-0.09146763214251408
5	4	-0.09316443078882486
5	5	-0.04629881075243958
5	6	0.07785656432839616
5	7	0.11139742332164815
5	8	0.08260255128313077
5	9	-0.07371178560068259
5	10	0.03833802154828092
5	11	0.08575771372610674
5	12	0.18925107650256479
5	13	0.1575206841910095
5	14	0.22223814960258892
5	15	0.27754801102303617
5	16	0.34047944710061984
5	17	0.24158681045323505
5	18	0.430597438578026
5	19	0.32107443881739073
5	20	0.28204979591673407
5	21	0.3872127812559114
5	22	0.3630205879113575
5	23	0.2773117374774259
5	24	0.41102289829095184
5	25	0.33821196672120024
5	26	1.3410474062829287
5	27	1.741036179400183
5	28	2.15388982919551
5	29	2.0452801189004646
5	30	2.558070746185223
5	31	4.882884868502163
5	32	5.7793975731987715
5	33	6.671534193395878
5	34	6.213273435636712
5	35	6.088853521136237
5	36	6.891923729833746
5	37	6.8953667993118515
5	38	6.992790229604374
5	39	7.00484193595452
5	40	6.819406627091851
5	41	7.2767192417995865
5	42	7.424635962917385
5	43	7.217796176614538
5	44	7.138546649358414
5	45	7.16425496429234
5	46	7.46091197946587
5	47	6.883203652480006
5	48	7.102008541792745
5	49	6.883743197900607
5	50	6.791940125755488
5	51	7.170346320273662
5	52	7.135983684367844
5	53	7.09322386244542
5	54	7.150906801986078
5	55	7.457320248574561
5	56	7.745969537278853
5	57	7.777792138936917
5	58	7.605965156054044
5	59	7.799599362367683
5	60	7.534124444715378
5	61	8.13223845120497
5	62	7.752248921894388
5	63	8.063332780175525
5	64	7.9585096035412874
5	65	7.656812831636781
5	66	7.883464575967631
5	67	7.718114619060145
5	68	7.601525718382412
5	69	7.494592028262641
5	70	7.440955281799948
5	71	7.31857082837783
5	72	7.042083693136179
5	73	7.033498585643557
6	1	-0.35159837754596523
6	2	-0.3065487910208269
6	3	-0.2985655107048224
6	4	-0.11587586293325103
6	5	-0.23296546438257337
6	6	-0.12944910060608095
6	7	-0.13570149836946044
6	8	-0.2984281715807029
6	9	-0.10037725790445506
6	10	-0.05228697352437095
6	11	0.033144465075210405
6	12	-0.01145889492145713
6	13	0.019668909176267846
6	14	0.004261912379643462
6	15	-0.22616405664467365
6	16	9.676001532739065E-4
6	17	0.035853662958004406
6	18	-0.022416609281428634
6	19	0.23756997805051938
6	20	0.4405645418902928
6	21	0.21013025993631018
6	22	0.3936959869119896
6	23	0.38518730272323154
6	24	0.3859476447718535
6	25	0.595145591143325
6	26	1.5115049405373
6	27	1.6794057121987436
6	28	1.8295542178387123
6	29	1.893566330008401
6	30	2.6869109928644725
6	31	4.5372699459294346
6	32	5.693531943708411
6	33	6.1713072479568005
6	34	5.933383786389854
6	35	6.096729099521632
6	36	6.727661205818826
6	37	6.82707689536101
6	38	6.856362828570909
6	39	6.761315030914268
6	40	7.028678715027301
6	41	7.145944910967174
6	42	6.994525285684098
6	43	7.06679767449679
6	44	6.937166172425954
6	45	7.093074720168666
6	46	7.070658898920097
6	47	7.1484549568624836
6	48	7.352185839680434
6	49	7.2283817747946735
6	50	7.361403108270963
6	51	7.219992219938089
6	52	7.105773335621194
6	53	6.993518119681294
6	54	6.929948999514046
6	55	7.043583476624125
6	56	7.466450790810502
6	57	7.567340533265202
6	58	7.661590453801001
6	59	7.445511129902442
6	60	7.680336676782538
6	61	7.585423136726213
6	62	7.454757602602651
6	63	7.564193518337859
6	64	6.99932305742329
6	65	7.397692123362837
6	66	7.657288128257738
6	67	7.338512045104277
6	68	7.213627122373797
6	69	7.608031860182255
6	70	7.288974786298926
6	71	7.527472934851847
6	72	6.807926605977203
6	73	6.854592410688966
7	1	-0.2365230432946177
7	2	-0.1658878260493779
7	3	-0.09043332278304916
7	4	-0.052651298425427484
7	5	-0.29684573927919544
7	6	-0.11205894176520559
7	7	0.060750552923956036
7	8	-0.14545759231538824
7	9	-0.13662323447563907
7	10	-0.06207288902267294
7	11	0.04882260061852506
7	12	0.12930762213154168
7	13	0.20649875776694415
7	14	0.12911536996085005
7	15	0.12155599564549746
7	16	0.18385722737277632
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119	15	-2.709460371284102
119	16	-2.8010534409011676
119	17	-2.8474971157588023
119	18	-2.8312311786265205
119	19	-2.9572570666170606
119	20	-3.0161302049874443
119	21	-4.012663902440746
119	22	-4.081067805004217
119	23	-4.147388988950571
119	24	-4.596780755294866
119	25	-4.937169843643488
119	26	-14.930244615815063
119	27	-14.7583972842075
119	28	-14.526491012267739
119	29	-14.69804173452386
119	30	-13.858563958237193
119	31	-12.737430256746398
119	32	-11.788520236129909
119	33	-11.238225837256389
119	34	-8.11918582345069
119	35	-7.697867090098512
119	36	-3.4570653190160456
119	37	-3.500267287985073
119	38	-3.2487721536919256
119	39	-3.308572482967529
119	40	-3.5794162103266522
119	41	-10.250574948691787
119	42	-10.240555026390716
119	43	-10.375041270440633
119	44	-10.406472485578774
119	45	-10.185339602913345
119	46	-10.446372052736304
119	47	-10.338924262273078
119	48	-10.28823425761755
119	49	-10.32657319020447
119	50	-10.259474477273077
119	51	-9.866857362682719
119	52	-9.791680396131664
119	53	-9.853067505495115
119	54	-9.737799868016793
119	55	-9.768167023891252
119	56	-10.015709783731209
119	57	-10.041734730733104
119	58	-9.773434283604654
119	59	-9.353031205035467
119	60	-8.998237251360793
119	61	-8.336664253156801
119	62	-8.638698915811695
119	63	-8.217132394934527
119	64	-8.64465865357342
119	65	-9.099270976854632
119	66	-9.13840650021369
119	67	-9.37733708858325
119	68	-9.122659303291128
119	69	-8.904192565198317
119	70	-8.71716700814143
119	71	-8.26637114120324
119	72	-8.19228599053517
119	73	-8.06553347890981
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	8335436.0
5	1028653.0
6	510566.0
7	528906.0
8	366112.0
9	405371.0
10	399543.0
11	465942.0
12	509329.0
13	609736.0
14	794976.0
15	1112301.0
16	1319050.0
17	1738917.0
18	2001389.0
19	1575902.0
20	1073744.0
21	686878.0
22	618469.0
23	588823.0
24	583060.0
25	593513.0
26	613824.0
27	643586.0
28	687046.0
29	738145.0
30	766631.0
31	765228.0
32	818536.0
33	933469.0
34	1116330.0
35	1371761.0
36	1685531.0
37	2025134.0
38	2220237.0
39	1148634.0
40	5.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.633468737364552	30.302056254755215	24.65009718651017	27.41437782137006
2	24.221159188178024	30.92375411638462	24.572103440593608	20.28298325484375
3	23.851764287731466	28.6145644797544	24.982044367017945	22.551626865496186
4	26.692309788973596	27.081110965427552	23.661334772643826	22.565244472955023
5	26.559561102694868	29.090078564866978	24.55780356376078	19.792556768677372
6	26.135549128071023	27.46249508283392	21.806061027594122	24.59589476150093
7	24.230742429314372	26.741022169368982	26.144038464442836	22.884196936873806
8	24.642971880010627	26.266694040126946	25.504898659896654	23.585435419965773
9	23.833525030596	26.16073928124238	25.83029215156223	24.175443536599392
10	23.703171055624725	27.84167036691731	24.265629297182024	24.189529280275945
11	24.474869219372323	27.072705215020402	24.45223099832187	24.000194567285405
12	24.26040657884096	26.46735582227183	25.432876478757805	23.8393611201294
13	23.876101731169967	26.67961550764737	25.43335997302923	24.010922788153433
14	23.833780032352042	26.80746094999561	25.40444088789503	23.95431812975732
15	23.381121240138206	26.690418011040716	25.606639876361758	24.32182087245932
16	23.552655854411498	26.405304624574615	25.585542172934712	24.45649734807918
17	24.045161208318127	26.244196514951145	25.559535065698473	24.15110721103225
18	23.55129541795361	26.345799686729404	25.554942370275285	24.5479625250417
19	23.910073685494087	26.508036985320405	25.417699741916422	24.16418958726908
20	24.013758479592134	26.331203208428928	25.469686062364367	24.18535224961457
21	23.682173751558015	26.639275011057684	25.382086885146705	24.2964643522376
22	24.05193606186746	26.275221907827063	25.356897071669337	24.315944958636145
23	23.941482389772403	26.29299974846353	25.58044746514701	24.185070396617053
24	23.609053799239963	26.539171663704714	25.372606019273398	24.479168517781925
25	23.72057990076395	26.363603290561244	25.43338606164865	24.482430747026157
26	23.388392499725743	26.35015439373525	26.229892719991003	24.031560386548005
27	23.179681772367793	26.130601720994573	26.297682264151256	24.39203424248638
28	23.032877560582428	26.042236846228146	25.937806237105924	24.9870793560835
29	23.13271569179913	26.322623284757814	26.22642453369973	24.318236489743324
30	22.844710286746487	25.59825384267092	26.24497406768936	25.312061802893236
31	22.457692696245196	26.269489383978158	26.593946184516604	24.678871735260046
32	21.387610453729764	25.667957303137122	26.759823264082154	26.18460897905096
33	21.68820857035356	25.117202046635956	24.87936630446157	28.31522307854891
34	22.49715410189885	25.154374895399904	25.223897018256164	27.12457398444508
35	21.78616638817155	25.8111013651433	26.624628332889593	25.77810391379555
36	22.255064450444518	25.12938143854467	25.35208906701079	27.263465044000025
37	22.027156091849157	25.276714311118443	25.751735981354578	26.944393615677825
38	22.19617765740132	25.071198262539635	25.277121390961927	27.455502689097116
39	21.975154821869424	25.19476443198585	25.75338879902308	27.076691947121645
40	22.35916791711541	26.200512047392337	23.777612553428398	27.66270748206385
41	22.40618548819125	25.158277335228036	23.439762568247108	28.9957746083336
42	22.878012597495488	24.698493913339288	23.346695958037433	29.07679753112779
43	22.83743652699215	24.50147964003627	23.5044126065089	29.156671226462684
44	23.16172602122064	24.342359453116156	23.6393064341166	28.856608091546605
45	23.264861171555985	23.95959209840093	23.72192017467331	29.053626555369778
46	23.389894892045014	23.844348780019825	23.634800476795427	29.13095585113974
47	23.235127361580933	23.96490010898479	23.833803597681598	28.966168931752684
48	23.15004007833715	23.804159308770835	23.799338186164523	29.246462426727494
49	23.229500817253154	23.944307043347486	23.61148606128766	29.214706078111696
50	23.28187042154396	24.02620944152489	23.673347092298226	29.018573044632923
51	23.273572689509397	23.723304702101426	23.630476282594103	29.37264632579507
52	23.29267374102566	23.952252373958853	23.326412269919345	29.428661615096146
53	23.139698285546558	24.084957359389836	23.614710754452084	29.160633600611526
54	22.848457393324125	24.270890871339464	23.319206828769918	29.561444906566493
55	22.692867227826675	24.313125806422974	23.343827290999403	29.650179674750948
56	22.854083543960915	24.087411423550222	23.793024669259545	29.26548036322932
57	22.44882086702253	24.414043373174948	23.72206283927637	29.415072920526153
58	22.96810429246792	24.464972548588637	23.246112876226285	29.320810282717158
59	22.48195702057566	24.521101981183673	23.285715948174175	29.711225050066492
60	22.305426382797023	24.35107831394178	23.89742337892865	29.446071924332546
61	23.425904462800606	24.96510870867163	24.155996697827074	27.452990130700687
62	22.40598511207554	25.092540328456447	23.675033065837557	28.826441493630455
63	22.456265943918957	24.503693449132243	24.269320004570556	28.770720602378248
64	22.167386401621783	25.229310766539736	23.76511543200584	28.838187399832638
65	23.28132987964164	24.376341756250124	22.928981016782096	29.413347347326145
66	22.374470120217143	24.472774749694715	24.0572261734081	29.095528956680038
67	22.384262005264347	24.42855545018428	23.37748668491947	29.8096958596319
68	23.13785718483162	24.001200287016513	22.944329994614453	29.91661253353741
69	22.846972282609688	23.59662412028992	23.337655378252236	30.218748218848155
70	22.910549655816205	23.55377527775469	23.0709010273289	30.464774039100213
71	22.780713194082416	23.17607304064765	22.9924089538871	31.050804811382832
72	23.044756406116782	23.37579940606193	22.956607178746154	30.622837009075138
73	22.48834637401711	23.513556564281583	23.429591269725094	30.568505791976214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	558.0
1	531.0
2	710.0
3	1577.0
4	2238.0
5	4095.0
6	9731.5
7	13511.0
8	20143.0
9	37455.5
10	62773.0
11	77410.0
12	97089.5
13	139733.0
14	187749.5
15	212802.0
16	239319.5
17	289179.5
18	331519.5
19	350517.0
20	363806.0
21	383208.5
22	389322.0
23	388938.0
24	386035.0
25	381824.0
26	380132.0
27	383626.0
28	399860.0
29	430563.5
30	448527.0
31	480504.5
32	560282.0
33	608082.0
34	668198.5
35	786065.5
36	910609.0
37	977402.0
38	1051952.0
39	1210262.0
40	1387806.0
41	1481590.0
42	1571801.5
43	1731535.0
44	1801057.0
45	1862790.0
46	1963384.5
47	2030687.5
48	2059129.0
49	2075729.0
50	2102850.0
51	2100009.0
52	2086647.0
53	2045131.5
54	1941422.0
55	1785286.0
56	1691344.0
57	1590437.5
58	1374222.0
59	1258913.0
60	1149107.5
61	944445.0
62	769441.0
63	689294.0
64	619644.5
65	494475.5
66	391951.0
67	344946.0
68	307823.0
69	240490.0
70	210280.0
71	185357.0
72	141373.0
73	106768.5
74	91225.0
75	79064.5
76	57499.5
77	40884.5
78	33674.0
79	28379.0
80	19133.5
81	15183.0
82	12457.5
83	7875.0
84	4951.5
85	3885.0
86	3201.0
87	2009.0
88	1233.5
89	966.0
90	793.5
91	493.0
92	304.5
93	244.0
94	196.0
95	137.5
96	127.0
97	109.5
98	106.5
99	178.5
100	236.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.0318430231011244
2	1.0957157746411956
3	1.9436832806626603
4	2.739595134573926
5	3.223252823120762
6	3.6187776658174062
7	3.8343031933741694
8	3.972688919110698
9	4.0012602006156825
10	4.028898680406981
11	4.065575187165093
12	4.105806490091169
13	4.185191299144604
14	4.2091904023016715
15	4.257787921633927
16	4.315604711789282
17	4.368518251485903
18	4.416760532859838
19	4.437233838865947
20	4.516086999274275
21	4.551112978647806
22	4.54445770424497
23	4.68603815502164
24	4.809112399779095
25	4.6794215459748125
26	5.916065293510046
27	6.281711482351694
28	7.914380305626924
29	6.241825751044938
30	8.819096471344029
31	7.904317646725903
32	12.567765567500008
33	12.36167197022439
34	9.638062543774922
35	11.231254038566227
36	9.607417832554022
37	9.98403531616287
38	10.500176736925727
39	9.934449413667668
40	11.518709211221179
41	9.474261596217543
42	8.275828886756978
43	8.162138240585657
44	6.893914563531083
45	6.613436071050782
46	6.609861942204814
47	7.103715201813947
48	6.8177389790262914
49	6.761142564169931
50	6.93341122469301
51	6.758278911240606
52	6.928063322640188
53	6.802149590800913
54	8.059812792495865
55	7.723119705549782
56	7.878336944073439
57	8.152877887821798
58	7.6441118837174225
59	8.629607227888993
60	9.221247589426504
61	7.152972448783084
62	11.608516750303457
63	10.66593753471575
64	11.613320920787421
65	7.360180091628678
66	11.148698670320156
67	10.875931499778654
68	7.595789855046722
69	7.147153312703916
70	7.0613718038159465
71	7.60972871588752
72	7.390397067348744
73	8.62397416883561
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
73	4.1380713E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.89397162354476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.90450021297586	99.79857309339432
2	0.08970316200965162	0.17921610240668753
3	0.0043484297335606124	0.013031457492338461
4	0.0010046985811967488	0.004014533262411348
5	2.0118287123294004E-4	0.0010048478015043285
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	2.2901554958902831E-4	0.003057378788390438
>50	1.3298278917523687E-5	0.0011025868543681487
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.283120351261226E-5	0.0	0.0	4.833169500970174E-6	0.0
2	6.766437301358243E-5	0.0	0.0	4.833169500970174E-6	0.0
3	7.974729676600788E-5	0.0	0.0	9.666339001940348E-6	0.0
4	1.498282545300754E-4	0.0	0.0	9.666339001940348E-6	0.0
5	5.123159671028385E-4	0.0	0.0	1.2082923752425436E-5	2.416584750485087E-6
6	5.461481536096297E-4	0.0	0.0	1.4499508502910523E-5	7.249754251455261E-6
7	5.606476621125402E-4	2.416584750485087E-6	0.0	1.9332678003880697E-5	9.666339001940348E-6
8	6.210622808746674E-4	4.833169500970174E-6	0.0	1.9332678003880697E-5	1.2082923752425436E-5
9	9.255519594357883E-4	4.833169500970174E-6	0.0	2.1749262754365782E-5	1.2082923752425436E-5
10	0.002165259936434638	4.833169500970174E-6	2.416584750485087E-6	2.1749262754365782E-5	1.2082923752425436E-5
11	0.0024794159539976994	4.833169500970174E-6	2.416584750485087E-6	2.8999017005821045E-5	1.2082923752425436E-5
12	0.002699325166291842	7.249754251455261E-6	2.416584750485087E-6	3.624877125727631E-5	1.4499508502910523E-5
13	0.0028249875733170667	7.249754251455261E-6	4.833169500970174E-6	3.624877125727631E-5	1.4499508502910523E-5
14	0.002994148505851023	7.249754251455261E-6	4.833169500970174E-6	3.624877125727631E-5	1.4499508502910523E-5
15	0.004857335348475025	7.249754251455261E-6	4.833169500970174E-6	3.624877125727631E-5	1.4499508502910523E-5
16	0.005055495298014802	1.2082923752425436E-5	4.833169500970174E-6	3.8665356007761394E-5	2.416584750485087E-5
17	0.005456648366595327	1.2082923752425436E-5	4.833169500970174E-6	4.3498525508731565E-5	2.6582432255335957E-5
18	0.005792553646912754	1.2082923752425436E-5	4.833169500970174E-6	4.591511025921666E-5	2.6582432255335957E-5
19	0.006181623791740852	1.6916093253395608E-5	4.833169500970174E-6	5.074827976018683E-5	2.6582432255335957E-5
20	0.04111335636000279	1.6916093253395608E-5	4.833169500970174E-6	5.074827976018683E-5	2.6582432255335957E-5
21	0.04194224492941917	2.1749262754365782E-5	4.833169500970174E-6	5.5581449261157005E-5	2.8999017005821045E-5
22	0.04219840291297059	2.416584750485087E-5	4.833169500970174E-6	6.524778826309735E-5	3.3832186506791216E-5
23	0.04246422723552395	2.416584750485087E-5	4.833169500970174E-6	7.974729676600788E-5	3.3832186506791216E-5
24	0.04274696765133071	2.416584750485087E-5	7.249754251455261E-6	8.941363576794822E-5	3.624877125727631E-5
25	0.04297654320262679	2.6582432255335957E-5	7.249754251455261E-6	9.424680526891839E-5	3.624877125727631E-5
26	0.04329794897444131	2.8999017005821045E-5	7.249754251455261E-6	9.666339001940348E-5	3.624877125727631E-5
27	0.04358068939024806	3.141560175630613E-5	7.249754251455261E-6	1.0149655952037366E-4	3.624877125727631E-5
28	0.04619060092077196	3.3832186506791216E-5	9.666339001940348E-6	1.0632972902134383E-4	3.624877125727631E-5
29	0.04660625349785539	3.3832186506791216E-5	9.666339001940348E-6	1.1599606802328418E-4	3.624877125727631E-5
30	0.047517305948788266	3.3832186506791216E-5	1.2082923752425436E-5	1.2082923752425435E-4	3.624877125727631E-5
31	0.06560302622141866	3.624877125727631E-5	1.2082923752425436E-5	1.2082923752425435E-4	4.108194075824648E-5
32	0.06612500852752344	3.8665356007761394E-5	1.2082923752425436E-5	1.2324582227473944E-4	4.108194075824648E-5
33	0.06663974107937676	3.8665356007761394E-5	1.2082923752425436E-5	1.2324582227473944E-4	4.3498525508731565E-5
34	0.06709405901246795	4.108194075824648E-5	1.2082923752425436E-5	1.2566240702522452E-4	4.3498525508731565E-5
35	0.06754354377605819	4.108194075824648E-5	1.2082923752425436E-5	1.2566240702522452E-4	4.3498525508731565E-5
36	0.068060692912662	4.108194075824648E-5	1.2082923752425436E-5	1.3291216127667979E-4	4.3498525508731565E-5
37	0.06865275617653084	4.591511025921666E-5	1.2082923752425436E-5	1.3774533077764997E-4	4.3498525508731565E-5
38	0.0693221501524152	4.591511025921666E-5	1.2082923752425436E-5	1.4016191552813505E-4	4.591511025921666E-5
39	0.06997462803504619	4.591511025921666E-5	1.2082923752425436E-5	1.4257850027862013E-4	4.833169500970174E-5
40	0.07082768245196741	4.591511025921666E-5	1.2082923752425436E-5	1.474116697795903E-4	5.074827976018683E-5
41	0.07172665197914788	5.074827976018683E-5	1.2082923752425436E-5	1.522448392805605E-4	5.316486451067191E-5
42	0.07271261855734579	5.074827976018683E-5	1.2082923752425436E-5	1.5466142403104558E-4	5.799803401164209E-5
43	0.07383149729682038	5.074827976018683E-5	1.2082923752425436E-5	1.5949459353201576E-4	5.799803401164209E-5
44	0.07505670576531633	5.074827976018683E-5	1.4499508502910523E-5	1.6674434778347102E-4	5.799803401164209E-5
45	0.0761127533012783	5.074827976018683E-5	1.4499508502910523E-5	1.7157751728444118E-4	6.0414618762127176E-5
46	0.07746604076154995	5.074827976018683E-5	1.6916093253395608E-5	1.7641068678541136E-4	6.283120351261226E-5
47	0.07899573890860702	5.074827976018683E-5	1.6916093253395608E-5	1.909101952883219E-4	6.524778826309735E-5
48	0.08060518435243008	5.074827976018683E-5	1.6916093253395608E-5	1.9815994953977715E-4	7.008095776406752E-5
49	0.0822218795505046	5.074827976018683E-5	1.6916093253395608E-5	2.0540970379123242E-4	7.249754251455262E-5
50	0.08395698740135289	5.074827976018683E-5	1.6916093253395608E-5	2.1024287329220257E-4	7.733071201552279E-5
51	0.08600141810026328	5.074827976018683E-5	1.6916093253395608E-5	2.1507604279317276E-4	7.733071201552279E-5
52	0.08807968098568046	5.074827976018683E-5	1.9332678003880697E-5	2.2715896654559818E-4	8.216388151649296E-5
53	0.09025460726111703	5.5581449261157005E-5	1.9332678003880697E-5	2.3440872079705344E-4	8.458046626697805E-5
54	0.09259627788433708	5.799803401164209E-5	1.9332678003880697E-5	2.4407505979899378E-4	8.699705101746313E-5
55	0.09529076988112796	5.799803401164209E-5	1.9332678003880697E-5	2.48908229299964E-4	9.424680526891839E-5
56	0.09807709209843726	6.0414618762127176E-5	1.9332678003880697E-5	2.5615798355141926E-4	9.424680526891839E-5
57	0.10109540645179313	6.0414618762127176E-5	2.416584750485087E-5	2.6340773780287447E-4	9.666339001940348E-5
58	0.1044230436532111	6.0414618762127176E-5	2.416584750485087E-5	2.6582432255335957E-4	9.666339001940348E-5
59	0.10802858810093485	6.0414618762127176E-5	2.6582432255335957E-5	2.7307407680481484E-4	9.907997476988858E-5
60	0.11186612468470516	6.283120351261226E-5	3.3832186506791216E-5	2.77907246305785E-4	9.907997476988858E-5
61	0.11611689726080844	6.283120351261226E-5	3.3832186506791216E-5	2.827404158067552E-4	9.907997476988858E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	27900	0.0	22.64628	1
ATCAACG	30870	0.0	20.738108	11
TCAACGC	32070	0.0	20.089664	12
GTATCAA	30500	0.0	19.764168	9
GGTATCA	30670	0.0	19.66789	8
AGTGGTA	30605	0.0	19.241499	5
CAACGCA	33730	0.0	18.697748	13
AACGCAG	33915	0.0	18.600594	14
GTGGTAT	32575	0.0	18.159647	6
GAGTACT	12475	0.0	17.961145	20
CGCAGAG	33800	0.0	17.92258	16
ACGCAGA	34210	0.0	17.843653	15
TGGTATC	33060	0.0	17.841473	7
TATCAAC	34695	0.0	17.601524	10
AGAGTAC	32545	0.0	17.178247	19
CAGTGGT	38390	0.0	15.645477	4
CAGAGTA	36250	0.0	15.46325	18
GTACTTT	14510	0.0	15.0479765	22
AGCAGTG	44365	0.0	14.7277975	2
GCAGTGG	44310	0.0	14.726397	3
>>END_MODULE