FastQCFastQC Report
Thu 26 May 2016
SRR805283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42531724
Sequences flagged as poor quality0
Sequence length73
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG183600.017.6745811
TCAACGC192000.016.85445412
GTATCAA188700.015.8972189
GGTATCA194200.015.2497658
CAACGCA215250.014.88405213
AGTGGTA199750.014.8245795
AACGCAG218150.014.70617314
GTGGTAT200800.014.65696056
TATCAAC213400.014.25691710
AGAGTAC207000.014.024199519
ACGCAGA224350.013.8041215
CGCAGAG229500.013.56379416
AAGCAGT265050.012.5350721
CAGAGTA246350.012.07149618
GAGTACT119650.011.27994920
TGGTATC274050.010.7141357
CAGTGGT298650.010.5469424
TACCGCC84950.010.3157766
GTACTTT135100.09.8352622
CGCCTAT45950.09.61858949
Produced by FastQC (version 0.11.5)