FastQCFastQC Report
Thu 26 May 2016
SRR805282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41544254
Sequences flagged as poor quality0
Sequence length73
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG218450.020.77711311
TCAACGC216850.020.76309412
GTATCAA219700.019.5583959
CAACGCA235250.019.07119813
GGTATCA227700.018.7412348
AGTGGTA232900.018.132925
AACGCAG248050.018.12229314
GTGGTAT241500.017.4548446
AGAGTAC238850.017.25255419
ACGCAGA257100.016.96715215
TATCAAC252950.016.92938610
CGCAGAG266450.016.39850816
AAGCAGT293100.015.709931
GAGTACT126050.015.08259520
CAGAGTA282750.014.82488318
GTACTTT144650.013.56422722
TGGTATC311650.013.4086287
CAGTGGT336450.012.9834354
AGTACTT168850.011.78874821
CTCGTAT43550.011.23840859
Produced by FastQC (version 0.11.5)