FastQCFastQC Report
Thu 26 May 2016
SRR805281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54753230
Sequences flagged as poor quality0
Sequence length73
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC221050.017.30487312
ATCAACG225250.016.71741911
GTATCAA228650.014.793549
CAACGCA265300.014.3320613
GGTATCA244200.014.0018268
AACGCAG282400.013.60680714
GTGGTAT242700.013.3198466
ACGCAGA277000.013.29930815
AGTGGTA240750.013.1655225
AGAGTAC242050.013.13830619
TATCAAC259400.013.11805510
CGCAGAG286750.012.90883616
CAGAGTA300350.011.00690118
AAGCAGT358550.010.9538381
GAGTACT153050.010.92997120
TGGTATC327500.09.9319417
GTACTTT189650.09.35404922
CAGTGGT363750.09.1470934
AGTACTT193550.08.91998621
GCAGTGG559950.07.3968823
Produced by FastQC (version 0.11.5)