FastQCFastQC Report
Thu 26 May 2016
SRR805280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40959018
Sequences flagged as poor quality0
Sequence length73
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG196150.019.55246211
TCAACGC198100.019.44369512
CAACGCA214650.017.72748813
GTATCAA205300.017.4771529
GGTATCA221000.016.2447438
AACGCAG235450.016.23843414
AGTGGTA218600.016.1140425
GTGGTAT216550.016.028196
ACGCAGA239150.015.46109515
TATCAAC234150.015.28103810
AGAGTAC230800.015.27845619
CGCAGAG251850.014.68444216
AAGCAGT273800.014.1390261
CAGAGTA276550.013.0115418
GAGTACT127250.012.16679220
CTCGTAT38550.012.11908159
GTACTTT142050.011.28916122
TGGTATC314350.011.1679767
CAGTGGT331000.010.9468254
GACCGAT50800.010.62588952
Produced by FastQC (version 0.11.5)