FastQCFastQC Report
Thu 26 May 2016
SRR805279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42058520
Sequences flagged as poor quality0
Sequence length73
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG207550.019.68014111
TCAACGC209150.019.4053712
GTATCAA213200.018.1797479
CAACGCA223650.017.8062713
GGTATCA225800.017.445458
AGTGGTA226750.016.9791415
GTGGTAT228550.016.8449576
TATCAAC243100.016.17517710
AACGCAG248000.016.1253214
ACGCAGA250400.015.46112815
AGAGTAC240750.015.29479119
AAGCAGT276850.014.95581
CGCAGAG262000.014.76778616
GAGTACT127400.013.92885720
CAGAGTA278200.013.44369518
CTCGTAT40900.012.89443859
GTACTTT143000.012.81342222
TGGTATC314650.012.0769267
CAGTGGT334350.011.85823254
GACCGAT51650.011.25318852