FastQCFastQC Report
Thu 26 May 2016
SRR805278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40962111
Sequences flagged as poor quality0
Sequence length73
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC247750.023.97502712
ATCAACG246950.023.88928211
GTATCAA254250.022.347169
CAACGCA262650.022.02570713
GGTATCA264400.021.8177038
GTGGTAT268350.020.9776736
AGTGGTA268750.020.9466825
AACGCAG282400.020.35836814
TATCAAC281350.020.10083610
CGCAGAG289600.019.62402216
AAGCAGT303350.019.5917221
ACGCAGA287650.019.43670315
AGAGTAC273300.018.92938819
GAGTACT140500.018.15447220
TGGTATC322550.017.339617
GTACTTT162250.016.90447422
CAGAGTA314700.016.6211518
CAGTGGT382900.014.9628974
AGTACTT177750.014.77659921
TACTTTT227250.012.293570523
Produced by FastQC (version 0.11.5)