FastQCFastQC Report
Thu 26 May 2016
SRR805277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10076923
Sequences flagged as poor quality0
Sequence length53
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5494815.452864927121107No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1763251.749790089693054No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC312470.3100847351914865No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCACCAAACTCATGCATA306610.30426946797152266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGCCACAACTAGATACATCAAC215980.21433129934604048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATGGCCTACCCATTCCAACTTG144240.14313893239037354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACAAACATACGAAAAACACA139790.13872290182231223No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG120370.11945114595000873No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA110050.10920992449778569No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAATAA109050.10821755807799663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATGCCACAACTAGATACATCAA103630.10283893208273993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1369100.046.418121
CAGTGGT1377400.045.868854
GCAGTGG1384750.045.7628443
GGTATCA1381750.045.663228
AGTGGTA1386750.045.537555
CGCAGAG1385900.045.44848616
ACGCAGA1387150.045.42446515
TCAACGC1391850.045.3723812
CAACGCA1393500.045.32203313
GTACATG648500.045.31495722
AGTACAT652250.045.22736721
GTATCAA1395150.045.226329
AACGCAG1396350.045.21101814
AGCAGTG1406700.045.1740572
AGAGTAC1369300.045.08814619
ATCAACG1401900.045.0772911
GCAGAGT1386300.045.05734617
CAGAGTA1379550.045.05635518
ACATGGG640200.044.9774324
GTGGTAT1406950.044.8587046