##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805277_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10076923
Sequences flagged as poor quality	0
Sequence length	53
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.87324394559728	37.0	35.0	37.0	35.0	37.0
2	35.96438724400296	37.0	35.0	37.0	35.0	37.0
3	37.62792402006049	39.0	37.0	39.0	35.0	39.0
4	37.536653698753085	39.0	37.0	39.0	35.0	39.0
5	37.52368733987547	39.0	37.0	39.0	35.0	39.0
6	37.50014493511561	39.0	37.0	39.0	35.0	39.0
7	37.39948404885102	39.0	37.0	39.0	34.0	39.0
8	38.33325391094087	40.0	38.0	40.0	34.0	40.0
9	38.32989306358697	40.0	38.0	40.0	34.0	40.0
10	37.97693462577813	40.0	38.0	40.0	34.0	40.0
11	38.20429738323891	40.0	38.0	40.0	34.0	40.0
12	38.2842445059866	40.0	38.0	40.0	34.0	40.0
13	38.32011746045891	40.0	38.0	40.0	34.0	40.0
14	38.32928593381134	40.0	38.0	40.0	34.0	40.0
15	38.340414628552786	40.0	38.0	40.0	34.0	40.0
16	38.22570768874586	40.0	38.0	40.0	34.0	40.0
17	38.153797245448835	40.0	38.0	40.0	34.0	40.0
18	38.09144745871334	40.0	38.0	40.0	34.0	40.0
19	38.000305053437444	40.0	38.0	40.0	34.0	40.0
20	37.95530232790307	40.0	38.0	40.0	34.0	40.0
21	37.73144808191945	40.0	38.0	40.0	33.0	40.0
22	37.80266029620351	40.0	38.0	40.0	33.0	40.0
23	37.336387605621276	39.0	37.0	40.0	32.0	40.0
24	37.57014983641336	40.0	37.0	40.0	33.0	40.0
25	37.65378905842587	40.0	37.0	40.0	33.0	40.0
26	37.69984428778507	40.0	38.0	40.0	33.0	40.0
27	37.69240114269009	40.0	38.0	40.0	33.0	40.0
28	37.64799443242744	40.0	37.0	40.0	33.0	40.0
29	37.60948952373656	40.0	37.0	40.0	33.0	40.0
30	37.52790291242674	40.0	37.0	40.0	33.0	40.0
31	37.36604020890107	40.0	37.0	40.0	32.0	40.0
32	37.32278285742582	40.0	37.0	40.0	32.0	40.0
33	37.2985897580045	40.0	37.0	40.0	32.0	40.0
34	37.209639688623206	40.0	37.0	40.0	32.0	40.0
35	37.13006281778674	40.0	37.0	40.0	31.0	40.0
36	37.00040766412525	39.0	36.0	40.0	31.0	40.0
37	36.8254848230953	39.0	36.0	40.0	31.0	40.0
38	36.612930256587255	39.0	35.0	40.0	31.0	40.0
39	36.515593599355675	39.0	35.0	40.0	31.0	40.0
40	36.45145100344619	38.0	35.0	40.0	30.0	40.0
41	36.3373244987582	38.0	35.0	40.0	30.0	40.0
42	36.258317643193266	38.0	35.0	40.0	30.0	40.0
43	36.20816731456616	38.0	35.0	40.0	30.0	40.0
44	36.08521301591766	38.0	35.0	40.0	30.0	40.0
45	36.03507350408453	38.0	35.0	40.0	30.0	40.0
46	35.89581412897568	38.0	35.0	40.0	30.0	40.0
47	35.72276179941039	38.0	35.0	40.0	29.0	40.0
48	35.65231668436883	38.0	35.0	40.0	29.0	40.0
49	35.85333925842244	38.0	35.0	40.0	29.0	40.0
50	35.83406065522184	38.0	35.0	40.0	28.0	40.0
51	35.789435128163625	38.0	35.0	40.0	28.0	40.0
52	35.584632531180404	38.0	35.0	40.0	28.0	40.0
53	34.97931045022374	38.0	34.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	7.0
11	12.0
12	25.0
13	49.0
14	86.0
15	202.0
16	519.0
17	1043.0
18	2051.0
19	4131.0
20	7113.0
21	11908.0
22	17814.0
23	25938.0
24	36340.0
25	49231.0
26	63451.0
27	80308.0
28	100488.0
29	126061.0
30	154794.0
31	189715.0
32	233688.0
33	294301.0
34	383482.0
35	527813.0
36	792562.0
37	1193637.0
38	1966762.0
39	3813386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.464632904310175	40.886459090736324	30.35236053704092	18.29654746791258
2	19.441281827796043	45.571083553977736	20.59067038618832	14.396964232037895
3	31.872963602083694	28.459659759234047	22.64341009651458	17.023966542167685
4	16.83308486132126	26.126268901727244	25.34632843775823	31.694317799193268
5	17.263454330255374	40.4998827519075	25.57949485175187	16.65716806608525
6	31.13209260406177	27.263282650864753	24.12197652001509	17.482648225058384
7	18.31990777343441	26.68634066172779	38.64601327210697	16.347738292730828
8	30.12763916127969	28.274146780718677	25.060526908858982	16.53768714914265
9	30.107315497002407	26.936347533865245	26.126249054398848	16.830087914733497
10	17.229823032288724	27.57934143190337	38.6896575472493	16.50117798855861
11	16.88820089227634	41.157335428681954	25.144679581257094	16.80978409778461
12	16.89004669381715	27.443675018654005	38.63082014222	17.03545814530884
13	16.842740586585805	27.61045211916376	25.818109357390146	29.72869793686029
14	17.214312345147423	41.401209476345116	24.775251334162224	16.609226844345244
15	17.130715397944392	40.60411099697795	25.099566603813482	17.165607001264174
16	17.08983982511328	27.407642193951464	25.5539513401065	29.948566640828755
17	30.424267407818835	27.497550591584357	25.17593912348045	16.902242877116358
18	17.498119217542897	27.14431776446044	25.291768131998232	30.065794885998436
19	17.57259631734806	40.31704916272557	25.389377293048682	16.72097722687769
20	30.696691837379326	27.90812235044368	24.800288738933503	16.59489707324349
21	17.747024562954387	40.614491149728934	24.862599426432055	16.77588486088462
22	30.388949086938545	27.21690936806801	25.89780630456341	16.496335240430042
23	17.477368835705107	27.791003265580176	38.05983235160178	16.671795547112943
24	17.494119980871144	40.070098779161064	25.21936507801042	17.216416161957376
25	17.088956618999667	27.19834219235376	25.858121571436044	29.85457961721053
26	17.26704669669501	33.858450640140845	32.12424070323848	16.750261959925663
27	17.624229142169686	26.898984938160194	38.59087739382349	16.88590852584663
28	23.638287203345705	27.092029977801758	32.60447658476699	16.665206234085545
29	23.708219264948237	27.934866625456994	31.937913984258888	16.419000125335877
30	23.7620253722292	27.05284142788428	32.09985826030426	17.085274939582252
31	19.043223809490257	29.719012440603148	33.39499567477096	17.842768075135634
32	18.595676477829592	30.023192595596893	33.91492621309104	17.466204713482476
33	19.435764270502016	28.943746022471345	33.56953308068346	18.050956626343183
34	18.57478716469303	29.062125412687976	34.08438270293422	18.278704719684768
35	18.397183346543382	29.658914730220726	32.86597506004561	19.07792686319028
36	18.417556629141654	29.26361549056195	33.089505596103095	19.2293222841933
37	18.231815406349735	29.13791243616727	34.039001786557264	18.591270370925727
38	18.57766502731042	29.925851373479784	33.37773842273083	18.11874517647897
39	18.81368945659305	29.043220832390997	33.35252239200398	18.790567319011963
40	18.031456626194327	29.237565872042488	33.82745903685083	18.903518464912356
41	18.268751284494282	30.027003282648877	33.604861325227944	18.099384107628886
42	18.4192039573985	29.822059769634045	33.340097964428224	18.418638308539222
43	18.39612151447421	29.70070328015804	34.05416514545164	17.849010059916107
44	18.54693143928955	29.347093353794605	33.41981475892988	18.68616044798596
45	18.347356628605777	28.93992541175516	34.19612316180247	18.516594797836603
46	18.025889450579307	29.345436101873556	33.91271323597491	18.715961211572225
47	18.528959683427175	29.958222366093302	32.93603612928272	18.576781821196807
48	19.015387931415177	29.324278849803655	33.29246437627835	18.367868842502816
49	18.527580294103664	29.583544500637743	33.647017050740594	18.241858154518003
50	18.60143220306437	29.5677559508989	33.45732620959791	18.373485636438822
51	18.591429149552894	29.715926181037606	33.209294146635834	18.483350522773666
52	18.407186400054858	29.520688011608303	33.5491002561	18.523025332236834
53	18.635887165159442	30.198682673272387	32.5084750573166	18.656955104251566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	180837.0
1	94912.5
2	8988.0
3	5940.0
4	2892.0
5	2363.5
6	1835.0
7	1697.0
8	1558.0
9	1557.0
10	1666.5
11	1776.0
12	2018.5
13	2261.0
14	2848.0
15	3435.0
16	7196.5
17	10958.0
18	17364.0
19	23770.0
20	41378.5
21	58987.0
22	359559.5
23	660132.0
24	413119.5
25	190980.5
26	215854.0
27	252919.0
28	289984.0
29	349806.5
30	409629.0
31	463476.0
32	517323.0
33	600325.0
34	683327.0
35	724805.0
36	766283.0
37	796445.5
38	826608.0
39	901930.0
40	977252.0
41	907858.0
42	758994.5
43	679525.0
44	629294.5
45	579064.0
46	543236.0
47	507408.0
48	466716.5
49	426025.0
50	363439.5
51	300854.0
52	259139.5
53	217425.0
54	198564.0
55	179703.0
56	158889.5
57	138076.0
58	118340.0
59	90002.5
60	81401.0
61	75686.0
62	69971.0
63	62732.5
64	55494.0
65	46242.5
66	36991.0
67	32164.0
68	27337.0
69	21181.5
70	15026.0
71	10958.0
72	6890.0
73	4580.5
74	2271.0
75	1813.5
76	1143.5
77	931.0
78	794.0
79	657.0
80	664.5
81	672.0
82	566.5
83	461.0
84	357.0
85	253.0
86	210.0
87	167.0
88	150.0
89	133.0
90	166.5
91	200.0
92	173.0
93	87.5
94	29.0
95	25.5
96	22.0
97	16.0
98	10.0
99	6.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.0076923E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.343739736681444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.97994286786862	11.041802654413143
2	12.058085289907124	3.4591607424226964
3	3.59434627967681	1.5466910267758012
4	1.49382188752837	0.8570796947066063
5	0.8698376775854351	0.6238362630222453
6	0.533401471472558	0.459058312917917
7	0.3571337745986387	0.358584373981507
8	0.29743129933923107	0.34130217178119376
9	0.22338288130963344	0.28837313200218456
>10	1.9290827891673425	5.578427324481782
>50	0.48489963158330235	5.095161883530745
>100	0.9141079215163876	29.338549399022572
>500	0.2003115282808679	19.86809518581062
>1k	0.06298243513056223	12.065780692666198
>5k	4.7921418034123435E-4	0.416960066353929
>10k+	7.530508548219397E-4	8.661137076110975
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	549481	5.452864927121107	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	176325	1.749790089693054	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	31247	0.3100847351914865	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCACCAAACTCATGCATA	30661	0.30426946797152266	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGCCACAACTAGATACATCAAC	21598	0.21433129934604048	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATGGCCTACCCATTCCAACTTG	14424	0.14313893239037354	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACAAACATACGAAAAACACA	13979	0.13872290182231223	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	12037	0.11945114595000873	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	11005	0.10920992449778569	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAATAA	10905	0.10821755807799663	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATGCCACAACTAGATACATCAA	10363	0.10283893208273993	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.961832098945283E-5	0.0	0.0	0.0	0.0
2	8.931297778101509E-5	0.0	0.0	0.0	0.0
3	8.931297778101509E-5	0.0	0.0	0.0	0.0
4	8.931297778101509E-5	0.0	0.0	0.0	0.0
5	8.931297778101509E-5	0.0	0.0	0.0	0.0
6	1.0916030617679623E-4	0.0	0.0	0.0	0.0
7	1.0916030617679623E-4	0.0	0.0	9.923664197890567E-6	0.0
8	1.0916030617679623E-4	0.0	0.0	9.923664197890567E-6	0.0
9	1.2900763457257737E-4	0.0	0.0	9.923664197890567E-6	0.0
10	1.488549629683585E-4	0.0	0.0	9.923664197890567E-6	0.0
11	1.6870229136413964E-4	0.0	0.0	9.923664197890567E-6	0.0
12	1.7862595556203018E-4	0.0	0.0	9.923664197890567E-6	0.0
13	2.381679407493736E-4	0.0	0.0	9.923664197890567E-6	0.0
14	2.877862617388264E-4	0.0	0.0	9.923664197890567E-6	0.0
15	3.374045827282793E-4	0.0	0.0	9.923664197890567E-6	0.0
16	4.267175605092944E-4	0.0	0.0	9.923664197890567E-6	0.0
17	4.8625954569663773E-4	0.0	0.0	9.923664197890567E-6	0.0
18	5.358778666860905E-4	0.0	0.0	1.9847328395781134E-5	0.0
19	5.755725234776528E-4	0.0	0.0	1.9847328395781134E-5	0.0
20	6.549618370607774E-4	0.0	0.0	1.9847328395781134E-5	0.0
21	7.740458074354642E-4	0.0	0.0	2.97709925936717E-5	0.0
22	8.534351210185887E-4	0.0	0.0	2.97709925936717E-5	0.0
23	9.923664197890565E-4	0.0	0.0	2.97709925936717E-5	0.0
24	0.0011511450469553056	0.0	0.0	2.97709925936717E-5	0.0
25	0.0013396946667152264	0.0	0.0	2.97709925936717E-5	0.0
26	0.0015381679506730377	0.0	0.0	3.969465679156227E-5	0.0
27	0.0016671755852456151	0.0	0.0	3.969465679156227E-5	0.0
28	0.0017763358914224113	0.0	0.0	3.969465679156227E-5	0.0
29	0.0018358778766097547	0.0	0.0	3.969465679156227E-5	0.0
30	0.0019748091753802226	0.0	0.0	3.969465679156227E-5	0.0
31	0.0021137404741506907	0.0	0.0	3.969465679156227E-5	0.0
32	0.002401526735889517	0.0	0.0	3.969465679156227E-5	0.0
33	0.002580152691451547	0.0	0.0	3.969465679156227E-5	0.0
34	0.0026893129976283433	0.0	0.0	3.969465679156227E-5	0.0
35	0.0027984733038051396	0.0	0.0	3.969465679156227E-5	0.0
36	0.002947328266773498	0.0	0.0	5.95419851873434E-5	0.0
37	0.0030862595655439663	0.0	0.0	5.95419851873434E-5	0.0
38	0.0031854962075228716	0.0	0.0	8.931297778101509E-5	0.0
39	0.0033145038420954493	0.0	0.0	8.931297778101509E-5	0.0
40	0.0034435114766680265	0.0	0.0	8.931297778101509E-5	0.0
41	0.003572519111240604	0.0	0.0	8.931297778101509E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	136910	0.0	46.41812	1
CAGTGGT	137740	0.0	45.86885	4
GCAGTGG	138475	0.0	45.762844	3
GGTATCA	138175	0.0	45.66322	8
AGTGGTA	138675	0.0	45.53755	5
CGCAGAG	138590	0.0	45.448486	16
ACGCAGA	138715	0.0	45.424465	15
TCAACGC	139185	0.0	45.37238	12
CAACGCA	139350	0.0	45.322033	13
GTACATG	64850	0.0	45.314957	22
AGTACAT	65225	0.0	45.227367	21
GTATCAA	139515	0.0	45.22632	9
AACGCAG	139635	0.0	45.211018	14
AGCAGTG	140670	0.0	45.174057	2
AGAGTAC	136930	0.0	45.088146	19
ATCAACG	140190	0.0	45.07729	11
GCAGAGT	138630	0.0	45.057346	17
CAGAGTA	137955	0.0	45.056355	18
ACATGGG	64020	0.0	44.97743	24
GTGGTAT	140695	0.0	44.858704	6
>>END_MODULE