FastQCFastQC Report
Thu 26 May 2016
SRR805276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39894663
Sequences flagged as poor quality0
Sequence length53
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9746522.443063624826208No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4457741.1173775299217343No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC608080.1524213902997501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2436100.046.252391
CAGTGGT2512650.044.5598874
GCAGTGG2544850.044.096733
GGTATCA2551150.043.804528
AGTGGTA2563800.043.647965
TCAACGC2583300.043.2811912
ACGCAGA2578050.043.25903715
CGCAGAG2576500.043.2321616
CAACGCA2587950.043.1834513
AACGCAG2606000.042.84917414
GTATCAA2614000.042.784569
AGCAGTG2639800.042.676212
AGAGTAC2579750.042.51908519
ATCAACG2634700.042.50015311
GTACATG1158850.042.46365422
GCAGAGT2621050.042.33685317
AGTACAT1175950.042.29380421
CAGAGTA2612900.042.25395218
GTGGTAT2656600.042.132996
TGGTATC2692850.041.4994937