##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805276_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39894663
Sequences flagged as poor quality	0
Sequence length	53
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.83747846171805	37.0	35.0	37.0	35.0	37.0
2	35.94031823254153	37.0	35.0	37.0	35.0	37.0
3	37.73310299675924	39.0	37.0	39.0	35.0	39.0
4	37.70083281565757	39.0	37.0	39.0	35.0	39.0
5	37.663700881493845	39.0	37.0	39.0	35.0	39.0
6	37.572907884946915	39.0	37.0	39.0	35.0	39.0
7	37.58301841025703	39.0	37.0	39.0	35.0	39.0
8	38.519235367397386	40.0	38.0	40.0	36.0	40.0
9	38.44129429041674	40.0	38.0	40.0	35.0	40.0
10	38.331853436135056	40.0	38.0	40.0	35.0	40.0
11	38.33647041961478	40.0	38.0	40.0	35.0	40.0
12	38.26795127959848	40.0	38.0	40.0	34.0	40.0
13	38.312120621247004	40.0	38.0	40.0	34.0	40.0
14	38.2557435314092	40.0	38.0	40.0	34.0	40.0
15	38.178706033937424	40.0	38.0	40.0	34.0	40.0
16	38.08961875426796	40.0	38.0	40.0	34.0	40.0
17	37.98802940132619	40.0	38.0	40.0	34.0	40.0
18	37.87164418458679	40.0	38.0	40.0	34.0	40.0
19	37.798992085733374	40.0	37.0	40.0	34.0	40.0
20	37.60892415609577	39.0	37.0	40.0	33.0	40.0
21	37.46429393325117	39.0	37.0	40.0	33.0	40.0
22	37.32242678676093	39.0	37.0	40.0	33.0	40.0
23	37.21243673621206	39.0	37.0	40.0	33.0	40.0
24	37.01873899774514	39.0	36.0	40.0	32.0	40.0
25	36.88636229362309	38.0	36.0	40.0	32.0	40.0
26	36.84648726071455	38.0	36.0	40.0	32.0	40.0
27	36.675132134842194	38.0	36.0	40.0	31.0	40.0
28	36.450336953592014	38.0	36.0	40.0	31.0	40.0
29	36.29134450891338	38.0	35.0	40.0	31.0	40.0
30	36.13223172232336	38.0	35.0	40.0	30.0	40.0
31	35.93070652081959	38.0	35.0	40.0	30.0	40.0
32	35.773829421744956	38.0	35.0	39.0	30.0	40.0
33	35.74671228580124	38.0	34.0	39.0	30.0	40.0
34	35.55111471927962	38.0	34.0	39.0	30.0	40.0
35	35.35812539637194	37.0	34.0	39.0	30.0	40.0
36	35.138714795009044	37.0	34.0	39.0	29.0	40.0
37	34.78219274593196	37.0	33.0	38.0	29.0	40.0
38	34.55639332007893	36.0	33.0	38.0	28.0	40.0
39	34.36113747846422	36.0	33.0	38.0	27.0	40.0
40	34.30642785477346	36.0	33.0	38.0	27.0	40.0
41	34.1178063341455	36.0	33.0	38.0	27.0	40.0
42	33.84269452282377	35.0	32.0	38.0	26.0	39.0
43	33.67695044823414	35.0	32.0	38.0	26.0	39.0
44	33.47055093559758	35.0	31.0	38.0	26.0	39.0
45	33.18608802385422	35.0	31.0	38.0	25.0	39.0
46	33.023879835756475	35.0	31.0	38.0	24.0	39.0
47	32.600845456446145	34.0	31.0	37.0	24.0	39.0
48	32.320740571238815	34.0	30.0	37.0	24.0	39.0
49	32.144817365671194	34.0	30.0	37.0	23.0	38.0
50	31.858619334621274	34.0	29.0	37.0	23.0	38.0
51	31.51624551885549	33.0	29.0	37.0	22.0	38.0
52	30.96256749931689	33.0	28.0	36.0	21.0	38.0
53	33.10804836225838	35.0	31.0	38.0	24.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	19.0
10	28.0
11	68.0
12	135.0
13	310.0
14	714.0
15	1582.0
16	3029.0
17	6332.0
18	12242.0
19	22439.0
20	38785.0
21	61869.0
22	94191.0
23	135623.0
24	188261.0
25	250100.0
26	323660.0
27	409009.0
28	509148.0
29	629774.0
30	775156.0
31	963807.0
32	1223214.0
33	1618426.0
34	2284637.0
35	3681121.0
36	6899135.0
37	1.1135677E7
38	8066562.0
39	559603.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.538468366056883	44.49454053540946	16.14285349396234	16.82413760457132
2	22.75443209032747	35.44319950766347	29.10099779511861	12.70137060689045
3	22.880015304302734	28.14527096017831	30.884116003185692	18.09059773233327
4	17.788973427347916	32.6134701275707	26.122930277666462	23.474626167414925
5	19.2296022152136	36.824276470263705	25.272448096628864	18.673673217893832
6	24.136606944141878	28.75773383522503	29.70614891520703	17.399510305426066
7	17.73907452232395	31.08911585492024	32.2118600174665	18.95994960528931
8	23.442473997085774	28.12455640996391	29.232223367822407	19.200746225127908
9	23.929188222494822	29.18762091059649	29.247538699599996	17.635652167308695
10	17.832836437294883	30.85380618455155	33.90135166701371	17.41200571113986
11	18.634815890035217	35.21087269242004	28.534252814718602	17.62005860282615
12	18.0477022703513	29.056673570597653	35.1667640355804	17.72886012347065
13	18.279515232400886	30.198222253437763	27.662514657662356	23.859747856499
14	18.857402054004066	34.8846260463461	28.047190171778112	18.21078172787172
15	18.111969011995413	35.096351108417686	28.62458319299501	18.16709668659189
16	18.8377503025906	29.58561148893525	27.304263730715057	24.272374477759094
17	25.275333194317245	28.48216063386724	28.077898539962604	18.16460763185291
18	19.107387872909214	28.91545919312566	28.038399522261916	23.938753411703214
19	19.09941938850317	35.64871321259187	26.80446755497095	18.44739984393401
20	25.546321822545536	28.905187142450607	27.315515862359835	18.23297517264402
21	19.14695707543638	34.52740533238744	28.217278085542418	18.108359506633757
22	24.820898975885573	29.549318413843977	27.368630736396998	18.261151873873455
23	19.230499578352124	28.649436141370586	33.81085835967583	18.30920592060146
24	18.769723659528093	34.5903661349389	28.091030622316577	18.548879583216433
25	18.793360906445056	29.38246401529949	27.428052218413274	24.39612285984218
26	19.39787785649424	31.028812049371112	31.292000135456714	18.281309958677934
27	18.79691526658591	28.735933926801188	34.28346292836213	18.183687878250783
28	21.593382052130632	29.705587436595216	30.520034722438936	18.180995788835215
29	22.088042202537217	28.062628327002038	31.30490913032653	18.54442034013422
30	21.48202379852162	28.744012701648842	31.223983518797993	18.549979981031548
31	19.948843783941726	30.47539215959789	30.94273787950032	18.633026176960062
32	20.221451676380873	29.30872733528292	31.8068033310621	18.663017657274107
33	19.883647093346795	29.415343099902863	31.81549872974237	18.88551107700797
34	19.400853191816662	30.51813221232123	31.322204676851133	18.75880991901097
35	19.812697753581727	29.152383114503312	31.829838993752123	19.20508013816284
36	19.418645045328496	29.819637779619796	31.726361493516066	19.03535568153565
37	19.507165657722187	30.41420603051591	31.059427673320616	19.019200638441287
38	19.983958756588567	29.404116034267542	31.470329753130137	19.141595456013754
39	19.667044185835085	29.758509302359563	31.768417244181258	18.806029267624094
40	19.443214747797217	30.519926938598278	30.976148363504162	19.060709950100343
41	19.74406701969133	29.399335946264294	31.6605857781027	19.196011255941677
42	19.337724447001843	30.0411661579896	31.85638139116503	18.764728003843523
43	19.46803009715861	30.46308976215691	30.95808078388831	19.11079935679617
44	19.958218471478254	29.22552071689389	31.439305553226504	19.376955258401356
45	19.048931432256992	29.722750133269706	32.071442739095204	19.1568756953781
46	19.235164362711874	30.40096616432128	31.43808985176789	18.925779621198956
47	19.64681591620413	29.689467485914093	31.514380758148025	19.14933583973375
48	19.514332029825642	30.04833253009306	31.541299145702773	18.896036294378522
49	19.19083261838808	30.868462280280447	31.111068666001767	18.829636435329707
50	19.597305534326733	29.545195055288474	31.691905756917908	19.165593653466882
51	19.090435730714155	29.96185981067192	31.738683943764606	19.209020514849318
52	19.046231823038585	30.738469955241882	30.862682058499907	19.352616163219626
53	19.778394919641258	29.603095030530774	31.083107532453653	19.53540251737432
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	474024.0
1	265437.5
2	56851.0
3	39239.5
4	21628.0
5	17262.5
6	12897.0
7	11394.0
8	9603.0
9	9315.0
10	9603.5
11	9892.0
12	11244.0
13	12596.0
14	17209.5
15	21823.0
16	38888.5
17	55954.0
18	91417.0
19	126880.0
20	193251.0
21	259622.0
22	832394.0
23	1405166.0
24	1029081.5
25	800477.5
26	947958.0
27	1138749.5
28	1329541.0
29	1567300.5
30	1805060.0
31	2042840.0
32	2280620.0
33	2588856.0
34	2897092.0
35	3073643.0
36	3250194.0
37	3366409.0
38	3482624.0
39	3743372.0
40	4004120.0
41	3707710.5
42	3048375.5
43	2685450.0
44	2435927.0
45	2186404.0
46	2029100.5
47	1871797.0
48	1715881.5
49	1559966.0
50	1364450.0
51	1168934.0
52	1029587.5
53	890241.0
54	804803.5
55	719366.0
56	642813.0
57	566260.0
58	492634.5
59	374467.5
60	329926.0
61	311665.5
62	293405.0
63	272212.5
64	251020.0
65	220861.0
66	190702.0
67	151267.5
68	111833.0
69	85344.5
70	58856.0
71	42349.5
72	25843.0
73	17187.0
74	8531.0
75	6637.5
76	4157.5
77	3571.0
78	3362.5
79	3154.0
80	3312.0
81	3470.0
82	2821.0
83	2172.0
84	1560.0
85	948.0
86	659.0
87	370.0
88	325.0
89	280.0
90	237.5
91	195.0
92	152.0
93	68.5
94	28.0
95	26.0
96	24.0
97	16.0
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	3.9894663E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.636231265762017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.99643853182246	7.232258454726353
2	14.454082622346123	3.074739310114106
3	6.454662228367949	2.0595984060990093
4	3.271135452272506	1.391702126880136
5	1.8744589786970238	0.9968589597802813
6	1.1762964222489423	0.7506816470476919
7	0.7781313678129198	0.579346962824136
8	0.5668199354162231	0.4823062335304992
9	0.41340259169834487	0.39573410140520865
>10	2.1400751586286555	4.121878998488457
>50	0.1892874696336212	1.410957192900704
>100	0.32097106961763466	8.820669521334686
>500	0.153780094163366	11.721004465367926
>1k	0.1961795493952916	42.942881741754654
>5k	0.013448380910202265	9.076282183417892
>10k+	8.301469697655719E-4	4.9430996943284295
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	974652	2.443063624826208	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	445774	1.1173775299217343	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	60808	0.1524213902997501	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0527723971499647E-4	0.0	0.0	5.013201891190308E-6	2.506600945595154E-6
2	1.1029044160618677E-4	0.0	0.0	5.013201891190308E-6	2.506600945595154E-6
3	1.1279704255178192E-4	0.0	0.0	7.519802836785461E-6	2.506600945595154E-6
4	1.1279704255178192E-4	0.0	0.0	1.0026403782380616E-5	2.506600945595154E-6
5	1.1279704255178192E-4	0.0	0.0	1.5039605673570923E-5	2.506600945595154E-6
6	1.1781024444297224E-4	0.0	0.0	1.5039605673570923E-5	2.506600945595154E-6
7	1.1781024444297224E-4	0.0	0.0	1.7546206619166076E-5	2.506600945595154E-6
8	1.1781024444297224E-4	0.0	0.0	1.7546206619166076E-5	2.506600945595154E-6
9	1.30343249170948E-4	0.0	0.0	2.506600945595154E-5	2.506600945595154E-6
10	1.4538285484451893E-4	0.0	0.0	2.506600945595154E-5	2.506600945595154E-6
11	1.529026576813044E-4	0.0	0.0	2.7572610401546694E-5	2.506600945595154E-6
12	1.6543566240928017E-4	0.0	0.0	3.0079211347141846E-5	2.506600945595154E-6
13	1.9802147470201716E-4	0.0	0.0	3.0079211347141846E-5	2.506600945595154E-6
14	2.782327049610621E-4	0.0	0.0	3.759901418392731E-5	2.506600945595154E-6
15	3.1081851725379907E-4	0.0	0.0	3.759901418392731E-5	2.506600945595154E-6
16	3.860165456216537E-4	0.0	0.0	4.010561512952246E-5	2.506600945595154E-6
17	4.411617664247471E-4	0.0	0.0	4.010561512952246E-5	2.506600945595154E-6
18	5.038267900646259E-4	0.0	0.0	4.2612216075117615E-5	2.506600945595154E-6
19	5.740116165412903E-4	0.0	0.0	4.511881702071277E-5	2.506600945595154E-6
20	6.567294477459303E-4	0.0	0.0	4.7625417966307925E-5	2.506600945595154E-6
21	7.49473682732951E-4	0.0	0.0	5.013201891190308E-5	2.506600945595154E-6
22	8.346981148831862E-4	0.0	0.0	5.2638619857498236E-5	2.506600945595154E-6
23	9.324555517613972E-4	0.0	0.0	5.514522080309339E-5	2.506600945595154E-6
24	0.001057785599041155	0.0	0.0	6.015842269428369E-5	2.506600945595154E-6
25	0.0011705826415929369	0.0	0.0	6.5171624585474E-5	2.506600945595154E-6
26	0.001336018304002217	0.0	0.0	7.269142742225946E-5	5.013201891190308E-6
27	0.0014513219474995942	0.0	0.0	7.269142742225946E-5	5.013201891190308E-6
28	0.0016092378070720888	0.0	0.0	8.522443215023523E-5	5.013201891190308E-6
29	0.0017370744552974416	0.0	0.0	8.773103309583039E-5	5.013201891190308E-6
30	0.0018924837139243412	0.0	0.0	8.773103309583039E-5	5.013201891190308E-6
31	0.0020629325782248117	0.0	0.0	9.274423498702069E-5	5.013201891190308E-6
32	0.002163196616048618	0.0	0.0	9.525083593261585E-5	5.013201891190308E-6
33	0.002328632278457898	0.0	0.0	1.0277063876940131E-4	5.013201891190308E-6
34	0.00244142932100968	0.0	0.0	1.1279704255178192E-4	5.013201891190308E-6
35	0.002604358382473365	0.0	0.0	1.1530364349737708E-4	1.0026403782380616E-5
36	0.0027597676411002647	0.0	0.0	1.2282344633416254E-4	1.0026403782380616E-5
37	0.002930216505400735	0.0	0.0	1.2783664822535286E-4	1.0026403782380616E-5
38	0.003155810590504299	0.0	0.0	1.529026576813044E-4	1.0026403782380616E-5
39	0.0033337792576415546	0.0	0.0	1.7295546524606563E-4	1.7546206619166076E-5
40	0.0035092413238332156	0.0	0.0	1.7295546524606563E-4	1.7546206619166076E-5
41	0.0037724344231207067	0.0	0.0	1.7796866713725592E-4	1.7546206619166076E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	243610	0.0	46.25239	1
CAGTGGT	251265	0.0	44.559887	4
GCAGTGG	254485	0.0	44.09673	3
GGTATCA	255115	0.0	43.80452	8
AGTGGTA	256380	0.0	43.64796	5
TCAACGC	258330	0.0	43.28119	12
ACGCAGA	257805	0.0	43.259037	15
CGCAGAG	257650	0.0	43.23216	16
CAACGCA	258795	0.0	43.18345	13
AACGCAG	260600	0.0	42.849174	14
GTATCAA	261400	0.0	42.78456	9
AGCAGTG	263980	0.0	42.67621	2
AGAGTAC	257975	0.0	42.519085	19
ATCAACG	263470	0.0	42.500153	11
GTACATG	115885	0.0	42.463654	22
GCAGAGT	262105	0.0	42.336853	17
AGTACAT	117595	0.0	42.293804	21
CAGAGTA	261290	0.0	42.253952	18
GTGGTAT	265660	0.0	42.13299	6
TGGTATC	269285	0.0	41.499493	7
>>END_MODULE