FastQCFastQC Report
Thu 26 May 2016
SRR805275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77081755
Sequences flagged as poor quality0
Sequence length53
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT53107726.889791235292969No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27206663.5295849192847255No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG4967890.6444962235226741No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGT4120050.534503917301831No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC3907800.5069682183546548No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3336350.4328326463246717No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3317580.4303975694378002No Hit
TAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG3249270.4215355501441294No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA3217570.4174230335051401No Hit
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3206900.4160387889455813No Hit
TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3037820.3941036371058236No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA2948870.3825639413632967No Hit
TGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG2714260.3521274262631929No Hit
TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT2625970.3406733538954841No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC2606900.33819935729278605No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAATAAGCAGT2517370.32658441676632816No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT2491310.3232035907848751No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT2484300.3222941667583983No Hit
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG2362400.3064797888942721No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC2343670.304049901302844No Hit
TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG2283770.2962789313761733No Hit
TCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2207630.28640110749943354No Hit
AGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG2152300.27922301457718496No Hit
AAGCAGTGGTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2143170.2780385578922016No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2088750.2709785214412931No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCACCAAACTCATGCATA2029880.263341175872293No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGAAAGCAGT1700300.22058397606541263No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT1663330.21578776975173958No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC1643390.213200905973145No Hit
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1557310.20203354217869066No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1430010.18551860942968929No Hit
AAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGT1411580.1831276415540876No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1381890.17927588701113512No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC1338800.17368571849460357No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG1239990.16086686142524909No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCG1231980.15982770501268426No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA1164720.15110190472440593No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG1147820.14890942739951885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAATAA1124240.14585033773556402No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGCCACAACTAGATACATCAAC1121710.1455221147987614No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGACTAAGCAGT1080240.14014211274769237No Hit
ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG998990.12960135637804301No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC926840.12024116472179441No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATGGCCTACCCATTCCAACTTG908250.11782943966441864No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGA887570.11514657392011898No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG850540.11034258366328063No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACACAGAGTAAGCAGT843150.10938386133009037No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC830300.10771680016885968No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC821480.10657256052356359No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGTAGAGTAAGCAGT811880.10532712961711886No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGTATCAACGCAGAGTAAGCAGTG791090.10262999331034951No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT785450.10189830265281323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG6063700.044.2972122
AGTACAT6159100.044.25551621
ACATGGG5886950.044.1661724
GAGTACA6311100.043.9157520
CATGGGA2156850.043.5809925
ATGGGGG561900.043.57474526
AGAGTAC11035650.042.86458619
TACATGG6214850.042.77670723
CATGGGT716050.042.77620325
ATCCCCG479900.042.66628642
ATGGGTA490050.042.51627726
ATGGGCG128800.042.43866326
CCCCGAT482800.042.4051444
ATGGGAC266350.042.37676226
TCCCCGA483800.042.37577443
CCCGATC483500.042.30486345
TGCAATC521200.042.0538238
CGATCCC484000.041.87758647
CATGGGG2229100.041.68875525
GTACTTT4279600.041.6730222
Produced by FastQC (version 0.11.5)