FastQCFastQC Report
Thu 26 May 2016
SRR805274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42716150
Sequences flagged as poor quality0
Sequence length53
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5160201.208020853939318No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4301471.0069891598376726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1013500.045.483571
CAGTGGT1092900.041.8394134
GGTATCA1102200.041.3883138
GCAGTGG1111200.041.3132173
TCAACGC1107900.041.2941612
CAACGCA1112050.041.11258313
ACGCAGA1111900.040.9976615
CGCAGAG1111700.040.92893616
AACGCAG1133100.040.30941414
AGTGGTA1140800.040.105325
GTATCAA1159050.039.4272049
AGAGTAC1149600.038.9642919
ATCAACG1182500.038.81822211
GCAGAGT1168800.038.78063217
GTACATG476600.038.5634722
CAGAGTA1170100.038.5166218
AGCAGTG1204250.038.3200722
AGTACAT487650.038.0414221
GTACTTT691150.037.30962822
GTGGTAT1246450.036.6946536
Produced by FastQC (version 0.11.5)