##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805274_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42716150
Sequences flagged as poor quality	0
Sequence length	53
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.62075577972266	37.0	35.0	37.0	35.0	37.0
2	35.74729351779128	37.0	35.0	37.0	35.0	37.0
3	37.54152469265137	39.0	37.0	39.0	35.0	39.0
4	37.532307944419145	39.0	37.0	39.0	35.0	39.0
5	37.51162986364642	39.0	37.0	39.0	35.0	39.0
6	37.41981620534622	39.0	37.0	39.0	35.0	39.0
7	37.45553152613239	39.0	37.0	39.0	35.0	39.0
8	38.36505443491513	40.0	38.0	40.0	35.0	40.0
9	38.26645706600431	40.0	38.0	40.0	34.0	40.0
10	38.2304616403866	40.0	38.0	40.0	34.0	40.0
11	38.201227755778554	40.0	38.0	40.0	34.0	40.0
12	38.111729053297175	40.0	38.0	40.0	34.0	40.0
13	38.142957148525795	40.0	38.0	40.0	34.0	40.0
14	38.075101360960666	40.0	38.0	40.0	34.0	40.0
15	37.985975468294775	40.0	38.0	40.0	34.0	40.0
16	37.90089586725396	40.0	38.0	40.0	34.0	40.0
17	37.81810949722763	40.0	37.0	40.0	34.0	40.0
18	37.67301435171475	40.0	37.0	40.0	33.0	40.0
19	37.60621462842508	39.0	37.0	40.0	33.0	40.0
20	37.404204007149524	39.0	37.0	40.0	33.0	40.0
21	37.29500432506207	39.0	37.0	40.0	33.0	40.0
22	37.09747725391919	39.0	36.0	40.0	32.0	40.0
23	37.05695370486338	39.0	36.0	40.0	32.0	40.0
24	36.812999134987585	38.0	36.0	40.0	32.0	40.0
25	36.68049974073038	38.0	36.0	40.0	31.0	40.0
26	36.59606860637019	38.0	36.0	40.0	31.0	40.0
27	36.43761364261526	38.0	36.0	40.0	31.0	40.0
28	36.23685994641371	38.0	35.0	40.0	30.0	40.0
29	36.095855314676065	38.0	35.0	40.0	30.0	40.0
30	35.941781761699026	38.0	35.0	40.0	30.0	40.0
31	35.7215822821111	38.0	34.0	39.0	30.0	40.0
32	35.54411256632444	38.0	34.0	39.0	30.0	40.0
33	35.520355790491415	38.0	34.0	39.0	30.0	40.0
34	35.32735115407171	37.0	34.0	39.0	29.0	40.0
35	35.13103449163841	37.0	34.0	39.0	29.0	40.0
36	34.91515234870184	37.0	34.0	39.0	29.0	40.0
37	34.59565026342496	37.0	33.0	38.0	27.0	40.0
38	34.401431355587995	36.0	33.0	38.0	27.0	40.0
39	34.206061220404926	36.0	33.0	38.0	27.0	40.0
40	34.15363467915531	36.0	33.0	38.0	27.0	40.0
41	33.94837872795184	36.0	32.0	38.0	26.0	40.0
42	33.69244887472303	35.0	32.0	38.0	26.0	39.0
43	33.521642868095554	35.0	32.0	38.0	25.0	39.0
44	33.31022856694716	35.0	31.0	38.0	25.0	39.0
45	33.038973222071746	35.0	31.0	38.0	24.0	39.0
46	32.85818216295242	35.0	31.0	38.0	24.0	39.0
47	32.43870496287704	34.0	30.0	37.0	24.0	39.0
48	32.139278984646324	34.0	30.0	37.0	23.0	39.0
49	31.959970292266508	34.0	30.0	37.0	23.0	38.0
50	31.673733189905924	34.0	29.0	37.0	23.0	38.0
51	31.379475584761266	33.0	29.0	37.0	22.0	38.0
52	30.888546907902516	33.0	28.0	36.0	21.0	38.0
53	33.02283518060499	35.0	31.0	38.0	24.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	16.0
9	31.0
10	47.0
11	180.0
12	474.0
13	1007.0
14	1927.0
15	3514.0
16	6286.0
17	10947.0
18	19473.0
19	32766.0
20	52877.0
21	81230.0
22	120031.0
23	170158.0
24	231082.0
25	304430.0
26	390846.0
27	491427.0
28	607465.0
29	744974.0
30	912368.0
31	1124083.0
32	1410649.0
33	1844564.0
34	2567406.0
35	4045804.0
36	7319722.0
37	1.1389346E7
38	8258185.0
39	572834.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.0810021502406	34.48217828619855	24.902298076956843	20.534521486604014
2	21.046098489681302	29.566004895104076	31.66069975875635	17.72719685645827
3	18.788481639848158	30.19338119189112	30.56731236312261	20.450824805138105
4	18.25722823803175	32.14187374096214	28.99038888102041	20.610509139985698
5	21.262751909991888	33.175904195485785	27.651672727996317	17.90967116652601
6	22.096228241543304	30.195614539231645	29.46580625828873	18.24235096093632
7	19.81411480201282	31.13666844975495	30.662784450377668	18.386432297854558
8	21.30091312068152	29.704978093765472	29.741172835098666	19.252935950454336
9	20.964000735084973	30.017279178952222	30.47787078189397	18.540849304068836
10	18.963560620514723	30.85498810168988	31.425716034801827	18.755735242993577
11	19.208882354800235	32.35988496154265	29.630802401433648	18.800430282223466
12	18.957338149622565	30.27056277309636	31.9553143249099	18.816784752371177
13	19.42855571019392	30.80411507123184	28.598061857166435	21.169267361407805
14	19.74691305279151	32.321433462519444	28.98210395833894	18.949549526350104
15	19.285057290977768	32.62767594926041	29.091446677661725	18.995820082100096
16	19.635100541598437	30.5069089793907	28.585417927411527	21.272572551599335
17	22.075194042534264	29.819450020659634	29.07207929553576	19.033276641270337
18	19.61438940541224	30.116613973871708	28.832797431416456	21.4361991892996
19	19.84475660844903	32.58011314221904	28.38984084473905	19.185289404592876
20	22.366990002610255	29.896027614848247	28.75292131898591	18.984061063555586
21	19.81455491658307	32.265419519315294	28.812231439397046	19.10779412470459
22	22.04382885629908	30.42466373959264	28.537883212789545	18.99362419131874
23	20.046659167551383	29.945484319162656	31.06644910648549	18.941407406800472
24	19.62959676843536	32.47716378933963	28.770738935976205	19.12250050624881
25	19.62923156698345	30.259452221232486	28.70539128643382	21.40592492535025
26	19.911567404834003	30.55131607132197	30.443860226167384	19.09325629767664
27	19.509953495340753	30.05282779463973	31.317698341259685	19.119520368759826
28	20.503727512896177	30.346864125161094	30.088807160757696	19.060601201185033
29	20.777937618441737	29.54397341520713	30.624077778545118	19.054011187806015
30	20.464061016734888	30.05642596535502	30.27252221934795	19.20699079856214
31	19.98006140534669	30.74841716774569	30.13287714365644	19.138644283251182
32	20.247733000282096	30.167695356440127	30.42437345125907	19.16019819201871
33	19.895226980895984	30.24688320459592	30.444567218721723	19.413322595786372
34	19.811352380774018	30.67392075362597	30.067714435874954	19.447012429725056
35	20.263242356813524	29.868916089113835	30.347180164879088	19.52066138919355
36	19.69178870286765	30.616434299439437	30.273812129604377	19.417964868088532
37	19.870819350526673	30.865557874480732	29.875019167223638	19.38860360776896
38	20.208206966217695	30.283761528133972	30.246499274864426	19.261532230783907
39	19.887702894572662	30.527669277310803	30.1834645678508	19.40116326026573
40	19.890095432289662	30.72261428054729	29.966230570873076	19.421059716289975
41	20.04285966783055	30.070181886710294	30.498160531789498	19.388797913669652
42	19.749120648747606	30.498336109410612	30.361238079742677	19.391305162099112
43	19.936026537972175	30.7527410592949	29.90494695800066	19.406285444732262
44	20.0731971397235	30.126570395506146	30.247538694381397	19.552693770388952
45	19.62521669204739	30.493544010871766	30.521783915451184	19.359455381629665
46	19.828711154914476	30.60266901394437	30.26348582444813	19.30513400669302
47	19.976039507305785	30.45260633273364	30.18307127397951	19.388282885981063
48	19.837564949088343	30.683113997867316	30.169626710272347	19.309694342771998
49	19.867190746357057	30.842931771706954	30.009052782144458	19.28082469979153
50	20.083134833078358	30.251794695917116	30.361776517780747	19.30329395322378
51	19.786307988898812	30.521727730612426	30.27440207041131	19.417562210077453
52	19.766568382216093	30.86481576640217	29.901018701357685	19.467597150024055
53	20.218385786172206	30.325300852253772	30.144526133558387	19.311787228015636
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	549026.0
1	306074.5
2	63123.0
3	43955.0
4	24787.0
5	19669.5
6	14552.0
7	13289.5
8	11562.0
9	11097.0
10	11144.5
11	11192.0
12	12464.0
13	13736.0
14	18808.0
15	23880.0
16	43367.5
17	62855.0
18	99536.5
19	136218.0
20	200826.5
21	265435.0
22	549597.0
23	833759.0
24	726992.0
25	808793.5
26	997362.0
27	1202338.0
28	1407314.0
29	1669882.5
30	1932451.0
31	2185983.5
32	2439516.0
33	2796836.0
34	3154156.0
35	3347515.0
36	3540874.0
37	3698247.0
38	3855620.0
39	4127695.5
40	4399771.0
41	4076592.0
42	3381957.0
43	3010501.0
44	2719970.5
45	2429440.0
46	2235848.0
47	2042256.0
48	1834992.0
49	1627728.0
50	1426404.0
51	1225080.0
52	1085766.0
53	946452.0
54	867408.5
55	788365.0
56	701579.0
57	614793.0
58	541432.0
59	415266.0
60	362461.0
61	348381.0
62	334301.0
63	308921.0
64	283541.0
65	235683.5
66	187826.0
67	157398.5
68	126971.0
69	101002.5
70	75034.0
71	56571.0
72	38108.0
73	24630.5
74	11153.0
75	8527.0
76	5075.5
77	4250.0
78	3756.5
79	3263.0
80	3721.5
81	4180.0
82	3277.0
83	2374.0
84	1581.5
85	789.0
86	565.5
87	342.0
88	301.5
89	261.0
90	225.5
91	190.0
92	142.0
93	56.5
94	19.0
95	15.5
96	12.0
97	8.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	4.271615E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.721449471224313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.73360820206645	10.85465587477772
2	12.097646269925225	3.56189776566899
3	5.27752921831803	2.330786391611365
4	2.714570691276201	1.598496610707562
5	1.5902431169556819	1.1705341846612665
6	0.9855631034462161	0.8705350456851894
7	0.6295620190899847	0.6487645817124617
8	0.4513209866170145	0.5315279279828387
9	0.330637636344829	0.4380718731061896
>10	1.6279142769035995	4.253115695286454
>50	0.11644698637344997	1.186935745783686
>100	0.18300362677837667	7.2858140922776204
>500	0.10488352024997964	11.180738656301601
>1k	0.15080257056990143	46.37453367192144
>5k	0.006143967183044076	5.371760647879829
>10k+	1.2380790293287788E-4	2.3418312346357837
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	516020	1.208020853939318	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	430147	1.0069891598376726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2641588719957206E-4	0.0	0.0	2.341034948140223E-6	0.0
2	1.2875692214771227E-4	0.0	0.0	7.0231048444206695E-6	0.0
3	1.2875692214771227E-4	0.0	0.0	1.6387244636981565E-5	0.0
4	1.2875692214771227E-4	0.0	0.0	1.8728279585121785E-5	0.0
5	1.2875692214771227E-4	0.0	0.0	2.8092419377682678E-5	0.0
6	1.3343899204399274E-4	0.0	0.0	2.8092419377682678E-5	0.0
7	1.3343899204399274E-4	0.0	0.0	3.0433454325822902E-5	0.0
8	1.3578002699213295E-4	0.0	0.0	3.745655917024357E-5	0.0
9	1.404620968884134E-4	0.0	0.0	4.447966401466424E-5	0.0
10	1.4514416678469385E-4	0.0	0.0	5.1502768859084915E-5	0.0
11	1.5450830657725474E-4	0.0	0.0	6.0866908651645804E-5	0.0
12	1.6621348131795586E-4	0.0	0.0	6.0866908651645804E-5	0.0
13	1.7557762111051675E-4	0.0	0.0	6.789001349606648E-5	0.0
14	2.013290055400592E-4	0.0	0.0	7.257208339234692E-5	0.0
15	2.223983200733212E-4	0.0	0.0	8.193622318490782E-5	0.0
16	2.3644452976216257E-4	0.0	0.0	8.427725813304804E-5	0.0
17	2.6219591419170504E-4	0.0	0.0	8.661829308118827E-5	0.0
18	2.8560626367310726E-4	0.0	0.0	8.661829308118827E-5	0.0
19	3.136986830507899E-4	0.0	0.0	9.832346782188938E-5	0.0
20	3.4413213737661283E-4	0.0	0.0	1.0300553771816983E-4	0.0
21	3.839297314949966E-4	0.0	0.0	1.1236967751073071E-4	0.0
22	4.330914654059413E-4	0.0	0.0	1.1471071245887094E-4	0.0
23	4.705480245761849E-4	0.0	2.341034948140223E-6	1.1939278235515139E-4	0.0
24	5.220507934352698E-4	0.0	2.341034948140223E-6	1.1939278235515139E-4	0.0
25	6.133511564127385E-4	0.0	2.341034948140223E-6	1.2407485225143184E-4	0.0
26	6.906053097013658E-4	0.0	2.341034948140223E-6	1.3343899204399274E-4	0.0
27	7.584953231974323E-4	0.0	2.341034948140223E-6	1.3578002699213295E-4	0.0
28	8.661829308118826E-4	0.0	2.341034948140223E-6	1.4514416678469385E-4	0.0
29	9.715295034781927E-4	0.0	2.341034948140223E-6	1.4514416678469385E-4	0.0
30	0.0010558067616112407	0.0	2.341034948140223E-6	1.615314114216754E-4	0.0
31	0.0011588122993294106	0.0	2.341034948140223E-6	1.6387244636981564E-4	0.0
32	0.0012758640467364216	0.0	2.341034948140223E-6	1.6855451626609608E-4	0.0
33	0.0014327133882618167	0.0	2.341034948140223E-6	1.7323658616237654E-4	0.0
34	0.001552106170616968	2.341034948140223E-6	2.341034948140223E-6	1.8962383079935808E-4	0.0
35	0.0016644758481276988	2.341034948140223E-6	2.341034948140223E-6	1.9664693564377876E-4	0.0
36	0.001769822420794009	2.341034948140223E-6	2.341034948140223E-6	2.013290055400592E-4	0.0
37	0.001898579342941721	2.341034948140223E-6	2.341034948140223E-6	2.223983200733212E-4	0.0
38	0.0020647928242596768	2.341034948140223E-6	2.341034948140223E-6	2.5517280934728436E-4	0.0
39	0.002254416655059035	2.341034948140223E-6	2.341034948140223E-6	2.7390108893240615E-4	0.0
40	0.002422971171325131	2.341034948140223E-6	2.341034948140223E-6	2.83265228724967E-4	0.0
41	0.0026477105263465928	2.341034948140223E-6	2.341034948140223E-6	2.926293685175279E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	101350	0.0	45.48357	1
CAGTGGT	109290	0.0	41.839413	4
GGTATCA	110220	0.0	41.388313	8
GCAGTGG	111120	0.0	41.313217	3
TCAACGC	110790	0.0	41.29416	12
CAACGCA	111205	0.0	41.112583	13
ACGCAGA	111190	0.0	40.99766	15
CGCAGAG	111170	0.0	40.928936	16
AACGCAG	113310	0.0	40.309414	14
AGTGGTA	114080	0.0	40.10532	5
GTATCAA	115905	0.0	39.427204	9
AGAGTAC	114960	0.0	38.96429	19
ATCAACG	118250	0.0	38.818222	11
GCAGAGT	116880	0.0	38.780632	17
GTACATG	47660	0.0	38.56347	22
CAGAGTA	117010	0.0	38.51662	18
AGCAGTG	120425	0.0	38.320072	2
AGTACAT	48765	0.0	38.04142	21
GTACTTT	69115	0.0	37.309628	22
GTGGTAT	124645	0.0	36.694653	6
>>END_MODULE