FastQCFastQC Report
Thu 26 May 2016
SRR805273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29370594
Sequences flagged as poor quality0
Sequence length53
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3972961.3526999147514687No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2396840.8160679351599085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT668500.043.449511
CAGTGGT713850.040.5739974
TCAACGC719650.040.19148312
CAACGCA721150.040.0850713
GCAGTGG725200.040.003793
CGCAGAG720150.039.96125816
ACGCAGA721650.039.94983715
GGTATCA723900.039.8970878
GTACATG329450.039.82409722
AACGCAG735100.039.2668314
AGTACAT337000.039.18293421
AGTGGTA750800.038.5897065
AGAGTAC748950.037.9915919
ACATGGG335050.037.9450824
GTATCAA762900.037.9345259
GCAGAGT759500.037.86918617
CAGAGTA759000.037.7455218
ATCAACG776200.037.44801311
AGCAGTG793200.036.7194862
GAGTACA373200.035.91747320
Produced by FastQC (version 0.11.5)