##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805273_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29370594
Sequences flagged as poor quality	0
Sequence length	53
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.4739332476558	37.0	35.0	37.0	33.0	37.0
2	35.72887259277085	37.0	35.0	37.0	35.0	37.0
3	37.61703661832648	39.0	37.0	39.0	35.0	39.0
4	37.639888794894645	39.0	37.0	39.0	35.0	39.0
5	37.608881420648146	39.0	37.0	39.0	35.0	39.0
6	37.515820415480874	39.0	37.0	39.0	35.0	39.0
7	37.560577869143536	39.0	37.0	39.0	35.0	39.0
8	38.445637633341704	40.0	38.0	40.0	35.0	40.0
9	38.35604785521192	40.0	38.0	40.0	35.0	40.0
10	38.317993977241315	40.0	38.0	40.0	35.0	40.0
11	38.26787027187805	40.0	38.0	40.0	35.0	40.0
12	38.194539511185916	40.0	38.0	40.0	34.0	40.0
13	38.24275116805605	40.0	38.0	40.0	34.0	40.0
14	38.16811015126218	40.0	38.0	40.0	34.0	40.0
15	38.08689446321719	40.0	38.0	40.0	34.0	40.0
16	38.0232505001431	40.0	38.0	40.0	34.0	40.0
17	37.95565850660017	40.0	38.0	40.0	34.0	40.0
18	37.80116146101778	40.0	37.0	40.0	34.0	40.0
19	37.73863085642735	40.0	37.0	40.0	34.0	40.0
20	37.53100213090685	39.0	37.0	40.0	33.0	40.0
21	37.428738281561486	39.0	37.0	40.0	33.0	40.0
22	37.24330794944086	39.0	37.0	40.0	33.0	40.0
23	37.193316280903275	39.0	37.0	40.0	33.0	40.0
24	36.953335809279174	39.0	36.0	40.0	32.0	40.0
25	36.832044186780834	38.0	36.0	40.0	32.0	40.0
26	36.750841811370925	38.0	36.0	40.0	32.0	40.0
27	36.60198190067249	38.0	36.0	40.0	31.0	40.0
28	36.395576779958894	38.0	35.0	40.0	31.0	40.0
29	36.26098832049498	38.0	35.0	40.0	30.0	40.0
30	36.10961753105845	38.0	35.0	40.0	30.0	40.0
31	35.89257650015522	38.0	35.0	40.0	30.0	40.0
32	35.72390221321366	38.0	35.0	39.0	30.0	40.0
33	35.7198070968534	38.0	34.0	39.0	30.0	40.0
34	35.523793900797514	38.0	34.0	39.0	30.0	40.0
35	35.34640528550427	37.0	34.0	39.0	30.0	40.0
36	35.13741564096389	37.0	34.0	39.0	29.0	40.0
37	34.80659802113638	37.0	33.0	38.0	29.0	40.0
38	34.621881293922755	36.0	33.0	38.0	28.0	40.0
39	34.432487609886266	36.0	33.0	38.0	27.0	40.0
40	34.38515233978584	36.0	33.0	38.0	27.0	40.0
41	34.209909408029	36.0	33.0	38.0	27.0	40.0
42	33.94436860895629	36.0	32.0	38.0	27.0	39.0
43	33.7814707118283	36.0	32.0	38.0	26.0	39.0
44	33.5866334879029	35.0	32.0	38.0	26.0	39.0
45	33.315405231504684	35.0	31.0	38.0	25.0	39.0
46	33.138825384328285	35.0	31.0	38.0	24.0	39.0
47	32.73649634052345	35.0	31.0	38.0	24.0	39.0
48	32.44904631482768	34.0	30.0	37.0	24.0	39.0
49	32.27881778625247	34.0	30.0	37.0	23.0	39.0
50	32.01279126326148	34.0	30.0	37.0	23.0	38.0
51	31.702869032883708	34.0	29.0	37.0	23.0	38.0
52	31.181846645661984	33.0	29.0	36.0	22.0	38.0
53	33.382699171831526	35.0	31.0	38.0	25.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	35.0
10	53.0
11	111.0
12	261.0
13	651.0
14	1272.0
15	2289.0
16	4054.0
17	7072.0
18	11981.0
19	20039.0
20	32266.0
21	49568.0
22	73574.0
23	104880.0
24	143016.0
25	189354.0
26	242305.0
27	306544.0
28	378493.0
29	467045.0
30	571836.0
31	709396.0
32	895506.0
33	1173105.0
34	1651197.0
35	2657103.0
36	4984682.0
37	8137772.0
38	6105477.0
39	449643.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	12.21473763860547	33.14731394264617	28.754617628775232	25.88333078997313
2	20.27699541929591	30.890233272095212	32.112813244430804	16.71995806417807
3	14.897958822351361	30.891578154667215	34.708089322265664	19.502373700715754
4	14.31820888607156	32.047305546493206	33.54810256816733	20.086382999267908
5	19.221681386491536	33.67201902692196	31.25155044531956	15.854749141266941
6	20.160351540728115	31.832372882890965	30.889599985618265	17.11767559076265
7	19.55189942702555	32.37337317726703	31.67374483471461	16.40098256099281
8	19.546543730099568	30.103054776488346	31.83509669569502	18.515304797717064
9	19.38027879177384	29.18178297653769	33.31109340178819	18.126844829900275
10	18.629575554379322	31.00970991597923	31.795904434210627	18.564810095430825
11	18.69181127218605	32.43159127118778	30.346393402870913	18.530204053755263
12	18.550775649957913	30.857145756057914	32.154776985443334	18.437301608540842
13	18.80039947438584	31.432237972442778	28.941794639904117	20.825567913267264
14	19.624380085741542	32.609166842182354	29.083293310308946	18.68315976176716
15	18.956821234190905	33.129650016611855	29.195170516469638	18.718358232727606
16	18.99689192530461	31.370584469622916	28.53215702753577	21.100366577536704
17	21.539353272868773	30.111859501377463	29.317575259118016	19.031211966635745
18	19.31141739932124	30.526699596201563	29.016229634307024	21.145653370170177
19	19.1061508664074	33.23831312366376	28.252322714344828	19.403213295584013
20	21.742948065674124	30.289819810930616	28.927753384899198	19.03947873849606
21	19.41654635925988	32.75408049289027	28.86351225991548	18.965860887934376
22	21.2813775574304	31.078874332606283	28.7667454052853	18.87300270467802
23	19.96891857209289	30.2887983811291	31.212453517283308	18.529829529494705
24	19.160113002821802	32.88574619907244	29.09539725345698	18.858743544648775
25	19.120250002434407	30.961021762106682	28.707945096377692	21.21078313908122
26	19.374088927176615	31.12739224817857	30.503768497157395	18.994750327487417
27	19.129766323418586	30.273224981421894	31.65370438200875	18.943304313150765
28	19.892839075709535	30.66656057415795	30.27259509971096	19.16800525042156
29	20.44275645225289	30.029276901924423	30.833084274700063	18.694882371122628
30	20.070026503379538	30.300347347418306	30.53980794532109	19.089818203881066
31	19.21928783598997	31.343468913158517	30.30702409355425	19.13021915729726
32	19.907425093275265	30.68761224236731	30.676039442716075	18.728923221641345
33	19.610389902226697	30.817456398736777	30.61797115849955	18.954182540536973
34	19.17001746713056	31.249817419422975	30.04881344926153	19.531351664184932
35	19.877953438735354	30.34708116560394	30.52810916932766	19.246856226333044
36	19.204010650925206	31.010237654710014	30.519219325288415	19.266532369076362
37	19.116457093104756	31.521163650963274	29.888792851789102	19.473586404142864
38	19.889642000430772	30.96117497657691	30.314201340292946	18.83498168269937
39	19.24181376787953	31.091107656862505	30.379821395508717	19.287257179749243
40	19.185121009129062	31.49044925683151	30.034278503185874	19.290151230853553
41	19.617230077130888	30.70976364999632	30.596899061694156	19.076107211178638
42	19.222369149224562	31.11439625633721	30.392882077904176	19.270352516534054
43	19.177167475741214	31.46989808922489	30.047516233413596	19.3054182016203
44	19.610413735588732	30.681779878200626	30.39115586153961	19.316650524671036
45	19.06075171649576	31.316785761976757	30.506267595405117	19.116194926122365
46	19.15417849567496	31.21958309729793	30.296356961660358	19.329881445366752
47	19.57653290907225	30.970558511686892	30.268250618288484	19.18465796095237
48	19.350732232381816	31.242538029704132	30.313499958495903	19.09322977941815
49	19.3297146118325	31.390209540876157	30.054836480324504	19.225239366966836
50	19.755313767232625	30.944699313878367	30.439013252506914	18.860973666382097
51	19.41134387680413	31.145672436859805	30.25516269776498	19.187820988571087
52	19.268827181363783	31.352641352776185	29.796179811685114	19.58235165417492
53	19.876142103220655	30.87167389260156	30.12527768420346	19.126906319974328
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	429154.0
1	239076.0
2	48998.0
3	33740.5
4	18483.0
5	14513.0
6	10543.0
7	9382.5
8	7831.5
9	7441.0
10	7548.5
11	7656.0
12	8828.5
13	10001.0
14	14730.5
15	19460.0
16	36696.5
17	53933.0
18	89047.5
19	124162.0
20	173297.0
21	222432.0
22	395342.0
23	568252.0
24	531719.5
25	653522.0
26	811857.0
27	968575.0
28	1125293.0
29	1306078.0
30	1486863.0
31	1686695.0
32	1886527.0
33	2118075.0
34	2349623.0
35	2460962.5
36	2572302.0
37	2654606.5
38	2736911.0
39	2907139.0
40	3077367.0
41	2799093.0
42	2263683.5
43	2006548.0
44	1794088.5
45	1581629.0
46	1418523.0
47	1255417.0
48	1114801.0
49	974185.0
50	849810.5
51	725436.0
52	626463.5
53	527491.0
54	476540.5
55	425590.0
56	379858.5
57	334127.0
58	286126.0
59	213273.0
60	188421.0
61	179804.5
62	171188.0
63	155461.5
64	139735.0
65	114034.0
66	88333.0
67	73250.0
68	58167.0
69	44572.5
70	30978.0
71	24856.0
72	18734.0
73	11978.0
74	5222.0
75	3983.0
76	2319.5
77	1895.0
78	1748.5
79	1602.0
80	1642.5
81	1683.0
82	1346.5
83	1010.0
84	681.5
85	353.0
86	258.5
87	164.0
88	153.5
89	143.0
90	128.0
91	113.0
92	82.0
93	32.5
94	14.0
95	10.0
96	6.0
97	5.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.9370594E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.285961322110907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.9897403883678	9.564529063886601
2	13.343156312439291	3.5455331736389684
3	5.597877156538483	2.231195381630954
4	2.743076085582607	1.4577761110663163
5	1.5457234725430076	1.026821113544268
6	0.9530695022440416	0.7597466726458699
7	0.6472083662883419	0.601914972529745
8	0.42850656371478496	0.4554497305428229
9	0.3201309984961897	0.38279232576262096
>10	1.54149478371279	3.7804297492516086
>50	0.17953512683249523	1.7031611864353344
>100	0.3982766412845799	13.297049172364567
>500	0.14477710792850865	13.724285741249798
>1k	0.16299335534112183	41.82482416005153
>5k	0.004255753796923207	3.2503228645243203
>10k+	1.7838488969139192E-4	2.394168580874621
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	397296	1.3526999147514687	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	239684	0.8160679351599085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.150008610653227E-5	0.0	0.0	0.0	0.0
2	7.83096181166782E-5	0.0	0.0	0.0	0.0
3	7.83096181166782E-5	0.0	0.0	3.4047660050729654E-6	0.0
4	7.83096181166782E-5	0.0	0.0	1.0214298015218896E-5	0.0
5	3.0642894045656687E-4	0.0	0.0	1.0214298015218896E-5	0.0
6	3.0642894045656687E-4	0.0	0.0	1.0214298015218896E-5	0.0
7	3.234527704819317E-4	0.0	0.0	1.0214298015218896E-5	0.0
8	3.234527704819317E-4	0.0	0.0	1.0214298015218896E-5	0.0
9	3.336670684971506E-4	0.0	0.0	1.0214298015218896E-5	0.0
10	3.643099625428073E-4	0.0	0.0	1.0214298015218896E-5	3.4047660050729654E-6
11	3.813337925681721E-4	0.0	0.0	1.3619064020291861E-5	3.4047660050729654E-6
12	4.017623885986099E-4	0.0	0.0	1.7023830025364825E-5	3.4047660050729654E-6
13	4.6304817668992325E-4	0.0	0.0	2.0428596030437792E-5	3.4047660050729654E-6
14	5.107149007609448E-4	0.0	0.0	2.0428596030437792E-5	3.4047660050729654E-6
15	5.515720928218204E-4	0.0	0.0	2.7238128040583723E-5	3.4047660050729654E-6
16	5.924292848826959E-4	0.0	0.0	2.7238128040583723E-5	3.4047660050729654E-6
17	6.400960089537175E-4	0.0	0.0	3.404766005072965E-5	3.4047660050729654E-6
18	6.979770310399578E-4	0.0	0.0	3.404766005072965E-5	3.4047660050729654E-6
19	7.592628191312712E-4	0.0	0.0	5.107149007609448E-5	3.4047660050729654E-6
20	8.137390752124387E-4	0.0	0.0	5.107149007609448E-5	3.4047660050729654E-6
21	8.648105652885331E-4	0.0	0.0	5.4476256081167446E-5	3.4047660050729654E-6
22	9.260963533798465E-4	0.0	0.0	5.4476256081167446E-5	3.4047660050729654E-6
23	9.941916734813058E-4	0.0	0.0	5.4476256081167446E-5	3.4047660050729654E-6
24	0.001058882227577692	0.0	0.0	5.4476256081167446E-5	3.4047660050729654E-6
25	0.0011303823136842245	0.0	0.0	5.4476256081167446E-5	3.4047660050729654E-6
26	0.0011950728677806108	0.0	0.0	5.4476256081167446E-5	3.4047660050729654E-6
27	0.0012938110819277267	0.0	0.0	5.7881022086240406E-5	3.4047660050729654E-6
28	0.001385739764064697	0.0	0.0	6.469055409638633E-5	3.4047660050729654E-6
29	0.0014742636801965939	0.0	0.0	6.80953201014593E-5	3.4047660050729654E-6
30	0.001555978064318345	0.0	0.0	7.150008610653227E-5	3.4047660050729654E-6
31	0.0016308829164299503	0.0	0.0	7.150008610653227E-5	3.4047660050729654E-6
32	0.0017125973005517015	0.0	0.0	7.490485211160523E-5	3.4047660050729654E-6
33	0.0018113355146988175	0.0	0.0	8.171438412175117E-5	3.4047660050729654E-6
34	0.0019305023248763712	0.0	0.0	8.171438412175117E-5	3.4047660050729654E-6
35	0.0019917881129676847	0.0	0.0	8.852391613189709E-5	3.4047660050729654E-6
36	0.0021007406251300196	0.0	0.0	9.192868213697006E-5	3.4047660050729654E-6
37	0.0021824550092517706	0.0	0.0	9.873821414711599E-5	3.4047660050729654E-6
38	0.002243740797343084	0.0	0.0	1.0214298015218896E-4	3.4047660050729654E-6
39	0.002325455181464835	0.0	0.0	1.259763421876997E-4	3.4047660050729654E-6
40	0.0024037647995815135	0.0	0.0	1.2938110819277267E-4	3.4047660050729654E-6
41	0.0025025030137286295	0.0	0.0	1.4300017221306453E-4	3.4047660050729654E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	66850	0.0	43.44951	1
CAGTGGT	71385	0.0	40.573997	4
TCAACGC	71965	0.0	40.191483	12
CAACGCA	72115	0.0	40.08507	13
GCAGTGG	72520	0.0	40.00379	3
CGCAGAG	72015	0.0	39.961258	16
ACGCAGA	72165	0.0	39.949837	15
GGTATCA	72390	0.0	39.897087	8
GTACATG	32945	0.0	39.824097	22
AACGCAG	73510	0.0	39.26683	14
AGTACAT	33700	0.0	39.182934	21
AGTGGTA	75080	0.0	38.589706	5
AGAGTAC	74895	0.0	37.99159	19
ACATGGG	33505	0.0	37.94508	24
GTATCAA	76290	0.0	37.934525	9
GCAGAGT	75950	0.0	37.869186	17
CAGAGTA	75900	0.0	37.74552	18
ATCAACG	77620	0.0	37.448013	11
AGCAGTG	79320	0.0	36.719486	2
GAGTACA	37320	0.0	35.917473	20
>>END_MODULE