FastQCFastQC Report
Thu 26 May 2016
SRR805272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065581
Sequences flagged as poor quality0
Sequence length53
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.33287004929705016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG50500.044.06831411
TCAACGC50750.043.99014712
GTATCAA50300.043.6829039
CAACGCA51350.043.659213
GGTATCA50900.043.4911588
AACGCAG49850.043.32296814
AGTGGTA50600.043.1452565
TGGTATC51850.042.7849547
TATCAAC53450.042.73526410
AAGCAGT48150.042.509871
GCAGTGG50000.042.2533
GTGGTAT51800.042.2364856
CAGTGGT52550.041.8125574
AGAGTAC51200.041.40038719
ACATGGG22200.040.96621324
ACGCAGA53200.040.90413715
CGCAGAG53450.040.80074716
AGCAGTG50350.040.4190642
TACATGG24250.040.313423
GTACTTT18150.040.2672222
Produced by FastQC (version 0.11.5)