##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805272_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1065581
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.77415982454642	32.0	28.0	35.0	17.0	35.0
2	30.93762370012228	35.0	30.0	35.0	17.0	35.0
3	32.9468186838917	35.0	32.0	37.0	19.0	39.0
4	33.963840383790625	37.0	33.0	38.0	27.0	39.0
5	34.415099368325826	37.0	33.0	39.0	27.0	39.0
6	34.168519333584214	37.0	33.0	38.0	27.0	39.0
7	34.4532147251124	37.0	33.0	38.0	27.0	39.0
8	34.94744651040137	37.0	33.0	39.0	27.0	40.0
9	34.503957934685396	37.0	33.0	39.0	27.0	40.0
10	34.74478711613664	36.0	33.0	38.0	27.0	40.0
11	34.16954788045207	36.0	33.0	38.0	27.0	40.0
12	33.713448344142776	36.0	32.0	38.0	26.0	39.0
13	33.605367400507326	36.0	32.0	38.0	25.0	39.0
14	33.13792100272058	35.0	31.0	38.0	25.0	39.0
15	32.81692241134179	34.0	31.0	37.0	25.0	39.0
16	32.59275925527952	34.0	31.0	37.0	25.0	38.0
17	31.220384935542207	34.0	29.0	36.0	19.0	38.0
18	31.21685822100807	33.0	29.0	36.0	19.0	38.0
19	30.626765116870516	32.0	27.0	36.0	19.0	38.0
20	29.04876963834753	32.0	26.0	35.0	16.0	37.0
21	28.798264984079108	31.0	26.0	34.0	16.0	37.0
22	27.844392871119137	30.0	24.0	34.0	11.0	36.0
23	27.205708435116616	30.0	24.0	33.0	11.0	36.0
24	26.416638434806927	29.0	23.0	32.0	10.0	35.0
25	25.984675965506142	27.0	22.0	32.0	10.0	35.0
26	25.510173323285606	27.0	19.0	31.0	10.0	34.0
27	24.502339099514725	27.0	18.0	30.0	10.0	34.0
28	23.908618866139694	25.0	16.0	30.0	10.0	34.0
29	22.914663455898708	25.0	16.0	30.0	10.0	33.0
30	22.45430239465606	24.0	16.0	30.0	10.0	33.0
31	21.898915239667375	24.0	15.0	30.0	10.0	31.0
32	21.20353684984999	24.0	15.0	28.0	10.0	31.0
33	20.336032643224684	21.0	11.0	27.0	10.0	30.0
34	19.654783634467957	19.0	10.0	26.0	9.0	30.0
35	18.92993212153745	18.0	10.0	25.0	9.0	30.0
36	18.34662498674432	16.0	10.0	25.0	9.0	30.0
37	17.88100294581078	16.0	10.0	24.0	9.0	30.0
38	17.15080693067913	15.0	10.0	24.0	9.0	28.0
39	16.66094740803374	15.0	10.0	24.0	9.0	27.0
40	16.124236449411168	15.0	10.0	23.0	9.0	26.0
41	15.549252473533217	14.0	10.0	22.0	9.0	25.0
42	15.145042000561196	13.0	10.0	21.0	9.0	25.0
43	14.725370478640292	13.0	10.0	19.0	9.0	24.0
44	14.35159035305622	12.0	9.0	19.0	8.0	24.0
45	13.972928383670505	11.0	9.0	17.0	8.0	24.0
46	13.695711541403234	10.0	9.0	15.0	8.0	24.0
47	13.362394787444597	10.0	9.0	15.0	8.0	23.0
48	13.065954629446283	10.0	9.0	15.0	8.0	23.0
49	12.839440643179636	10.0	9.0	15.0	8.0	23.0
50	12.62652768771215	10.0	9.0	14.0	8.0	22.0
51	12.429682023234273	10.0	9.0	14.0	8.0	22.0
52	12.244323050054383	10.0	9.0	14.0	8.0	22.0
53	11.877523154035217	10.0	9.0	13.0	8.0	20.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
10	3.0
11	45.0
12	199.0
13	604.0
14	1587.0
15	3572.0
16	7196.0
17	13329.0
18	23221.0
19	37774.0
20	57070.0
21	80566.0
22	105734.0
23	133939.0
24	163393.0
25	178530.0
26	140836.0
27	67770.0
28	24914.0
29	10553.0
30	5817.0
31	3931.0
32	2501.0
33	1531.0
34	683.0
35	216.0
36	59.0
37	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.239685204597304	43.33082140165788	19.259915482727262	21.169577911017555
2	28.137701404210475	37.99551606119103	19.19863436003457	14.668148174563925
3	29.462706260715986	24.94479537454215	24.784976458852025	20.80752190588984
4	21.517650934091353	27.214730743134496	22.465959884795243	28.801658437978904
5	20.462451939364534	32.22861518739542	25.08753440611272	22.221398467127322
6	26.13607036912257	24.358730120000263	26.08398610710964	23.42121340376752
7	23.166047442662734	24.905380257343175	29.485323030346827	22.443249269647264
8	25.14834630121971	25.15106782121678	27.257899681019087	22.44268619654442
9	26.448482095683012	24.384819173765298	26.929534216544777	22.237164514006913
10	22.296850262908215	25.52438528840135	30.15838307927788	22.020381369412554
11	21.902511399884194	30.37375854111513	25.471456416734156	22.25227364226652
12	21.828842668928967	24.85883288084153	30.505799183731693	22.806525266497808
13	22.02507364526958	25.722493175084765	25.498390080153456	26.754043099492204
14	22.16452808374023	30.234304102644472	25.188230646004385	22.41293716761091
15	22.013155264592744	29.94150608916638	25.335568107914835	22.70977053832604
16	22.52245488611377	25.100766624029518	25.238156461123083	27.13862202873362
17	26.654942233391925	24.916266337331468	26.105852112603362	22.322939316673253
18	22.0625180066086	24.859583644978656	25.312388265181156	27.765510083231586
19	22.406837208996784	29.935969203655095	25.103488144026592	22.55370544332153
20	26.836439463541485	25.07486526129877	25.89751506455164	22.19118021060811
21	22.391634235220035	29.773147231416473	24.964502933141638	22.87071560022185
22	26.626413196181236	25.004199586892035	25.835201641170404	22.534185575756325
23	22.102026969324715	25.307414452772715	30.332091131504786	22.258467446397788
24	22.284931882231383	29.598969951603866	25.274005448670728	22.842092717494026
25	22.12060838171852	25.222108877692072	25.132861790891543	27.524420949697863
26	22.103622343116104	28.149338248335887	27.33513454162565	22.411904866922363
27	22.492893548214543	24.656595791403937	29.95267370570609	22.89783695467543
28	25.11221577712065	25.125260304003167	27.120415998408383	22.6421079204678
29	24.15677456711409	25.065480709584726	28.173550391758113	22.604194331543074
30	25.131172571583015	24.73420603407906	27.365165107110577	22.769456287227342
31	23.532983414681755	25.819529439807955	27.49589191248718	23.151595233023112
32	22.85222803334519	26.098344471232128	27.87193089966882	23.177496595753865
33	22.763637865164636	25.438328949183592	28.01035303745093	23.78768014820084
34	22.70977053832604	25.871050628718038	27.97375328576617	23.44542554718975
35	22.355316020086693	25.72352547577331	28.372033660510088	23.549124843629908
36	22.468775250309456	25.52156992288714	28.259325194424452	23.750329632378957
37	22.42485554828774	25.769979006757815	28.361992190176068	23.44317325477838
38	22.190147909919563	25.65792745929216	28.60852436370393	23.54340026708434
39	22.27554733051734	25.59289251591385	28.39493196669235	23.736628186876455
40	22.07143333073694	25.784712752948863	28.455368479730776	23.688485436583424
41	22.178886447862713	25.735631547484424	28.557566247896688	23.527915756756173
42	22.20225398163068	25.457098052611677	28.52030957759194	23.820338388165705
43	22.09573931967631	25.773545136409155	28.337029282616715	23.79368626129783
44	22.104185416218947	25.66205666204634	28.57821226166758	23.655545660067137
45	21.897256050924334	25.491070129816507	28.763463312502758	23.84821050675641
46	22.17804183820845	25.697248730973993	28.205927095171557	23.918782335646
47	22.098273148639098	25.64854290757812	28.433033246651355	23.820150697131425
48	22.153266621683382	25.503270047044758	28.182653406920732	24.160809924351128
49	22.40411568899971	25.92332258176525	28.08289562220047	23.589666107034564
50	22.219427711267375	25.693213373736956	28.219440849639778	23.867918065355894
51	22.16893882304583	25.815024854985214	27.979290171277455	24.036746150691503
52	22.459484544112556	25.875930595609343	27.788689925965272	23.87589493431283
53	22.359914450426572	25.8983596742059	27.689307523313573	24.05241835205395
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	268.0
1	245.0
2	222.0
3	211.0
4	200.0
5	190.5
6	181.0
7	172.0
8	170.5
9	178.0
10	182.0
11	186.0
12	214.0
13	242.0
14	304.0
15	366.0
16	518.0
17	670.0
18	1007.5
19	1345.0
20	1998.5
21	2652.0
22	5748.0
23	8844.0
24	9248.5
25	10805.5
26	11958.0
27	13674.5
28	15391.0
29	17556.5
30	19722.0
31	22444.5
32	25167.0
33	28391.5
34	31616.0
35	35327.0
36	39038.0
37	43046.0
38	47054.0
39	51771.0
40	56488.0
41	60944.0
42	70154.0
43	74908.0
44	78981.0
45	83054.0
46	85576.0
47	88098.0
48	88731.5
49	89365.0
50	87925.5
51	86486.0
52	82597.5
53	78709.0
54	72871.0
55	67033.0
56	59528.0
57	52023.0
58	45122.0
59	32297.5
60	26374.0
61	21896.5
62	17419.0
63	14230.5
64	11042.0
65	8810.5
66	6579.0
67	5306.5
68	4034.0
69	3187.5
70	2341.0
71	1781.5
72	1222.0
73	988.5
74	755.0
75	583.5
76	304.0
77	196.0
78	159.0
79	122.0
80	106.5
81	91.0
82	70.5
83	50.0
84	39.0
85	28.0
86	17.5
87	7.0
88	6.5
89	6.0
90	3.0
91	0.0
92	0.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1065581.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.21843793364606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.44149417807138	92.78226865556424
2	1.750556780112827	3.3337056423299303
3	0.44055376491309706	1.2584652396243547
4	0.1597073068238139	0.6082832112941237
5	0.08143863514241556	0.38772298128544724
6	0.040643933225891174	0.2322031099949264
7	0.023696461587155975	0.15794380398185476
8	0.01647038080649177	0.12546271460531616
9	0.009967224700981263	0.08541572099049795
>10	0.03369576070833798	0.5086959922412206
>50	8.888734772554682E-4	0.054059740414106466
>100	7.881782285893819E-4	0.13302450123177947
>500	0.0	0.0
>1k	9.852220173102897E-5	0.3327486864422125
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3547	0.33287004929705016	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	9.384551714041448E-5	0.0	0.0
8	0.0	0.0	9.384551714041448E-5	0.0	0.0
9	0.0	0.0	9.384551714041448E-5	0.0	0.0
10	0.0	0.0	9.384551714041448E-5	0.0	0.0
11	0.0	0.0	9.384551714041448E-5	0.0	0.0
12	0.0	0.0	9.384551714041448E-5	0.0	0.0
13	0.0	0.0	9.384551714041448E-5	0.0	0.0
14	0.0	0.0	9.384551714041448E-5	0.0	0.0
15	9.384551714041448E-5	0.0	9.384551714041448E-5	0.0	0.0
16	9.384551714041448E-5	0.0	9.384551714041448E-5	0.0	0.0
17	9.384551714041448E-5	0.0	9.384551714041448E-5	9.384551714041448E-5	0.0
18	2.8153655142124345E-4	0.0	9.384551714041448E-5	9.384551714041448E-5	0.0
19	2.8153655142124345E-4	0.0	9.384551714041448E-5	9.384551714041448E-5	0.0
20	3.7538206856165793E-4	0.0	9.384551714041448E-5	1.8769103428082896E-4	0.0
21	4.692275857020724E-4	0.0	9.384551714041448E-5	2.8153655142124345E-4	0.0
22	5.630731028424869E-4	0.0	9.384551714041448E-5	2.8153655142124345E-4	0.0
23	7.507641371233159E-4	0.0	9.384551714041448E-5	2.8153655142124345E-4	0.0
24	7.507641371233159E-4	0.0	9.384551714041448E-5	2.8153655142124345E-4	0.0
25	0.0013138372399658026	0.0	9.384551714041448E-5	2.8153655142124345E-4	0.0
26	0.0015953737913870461	0.0	9.384551714041448E-5	2.8153655142124345E-4	0.0
27	0.001783064825667875	0.0	9.384551714041448E-5	3.7538206856165793E-4	0.0
28	0.0018769103428082896	0.0	9.384551714041448E-5	3.7538206856165793E-4	0.0
29	0.0020646013770891187	0.0	9.384551714041448E-5	3.7538206856165793E-4	0.0
30	0.002346137928510362	0.0	9.384551714041448E-5	3.7538206856165793E-4	0.0
31	0.0024399834456507764	0.0	9.384551714041448E-5	4.692275857020724E-4	0.0
32	0.0024399834456507764	0.0	9.384551714041448E-5	5.630731028424869E-4	0.0
33	0.00272151999707202	0.0	9.384551714041448E-5	6.569186199829013E-4	0.0
34	0.002815365514212434	0.0	9.384551714041448E-5	8.446096542637303E-4	0.0
35	0.002909211031352849	0.0	9.384551714041448E-5	0.0010323006885445594	0.0
36	0.002909211031352849	0.0	9.384551714041448E-5	0.0011261462056849738	0.0
37	0.0030030565484932634	0.0	9.384551714041448E-5	0.0012199917228253882	0.0
38	0.0030030565484932634	0.0	9.384551714041448E-5	0.0013138372399658026	0.0
39	0.0031907475827740923	0.0	9.384551714041448E-5	0.0013138372399658026	0.0
40	0.003472284134195336	0.0	9.384551714041448E-5	0.001407682757106217	0.0
41	0.003472284134195336	0.0	9.384551714041448E-5	0.001407682757106217	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCAACG	5050	0.0	44.068314	11
TCAACGC	5075	0.0	43.990147	12
GTATCAA	5030	0.0	43.682903	9
CAACGCA	5135	0.0	43.6592	13
GGTATCA	5090	0.0	43.491158	8
AACGCAG	4985	0.0	43.322968	14
AGTGGTA	5060	0.0	43.145256	5
TGGTATC	5185	0.0	42.784954	7
TATCAAC	5345	0.0	42.735264	10
AAGCAGT	4815	0.0	42.50987	1
GCAGTGG	5000	0.0	42.253	3
GTGGTAT	5180	0.0	42.236485	6
CAGTGGT	5255	0.0	41.812557	4
AGAGTAC	5120	0.0	41.400387	19
ACATGGG	2220	0.0	40.966213	24
ACGCAGA	5320	0.0	40.904137	15
CGCAGAG	5345	0.0	40.800747	16
AGCAGTG	5035	0.0	40.419064	2
TACATGG	2425	0.0	40.3134	23
GTACTTT	1815	0.0	40.26722	22
>>END_MODULE