FastQCFastQC Report
Thu 26 May 2016
SRR805271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724208
Sequences flagged as poor quality0
Sequence length53
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.16964310570418417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG23400.037.7606824
GTATCAA54150.037.582649
AGTGGTA53750.037.2939575
CAACGCA57600.036.88194313
GTGGTAT54450.036.8145076
TCAACGC57650.036.6461412
ATCAACG57000.036.61052711
AGAGTAC55050.036.54132519
GGTATCA54800.036.407858
CATGGGG12750.036.12549225
GCAGTGG52950.036.037773
TATCAAC60250.036.00082810
CAGTGGT55950.035.995534
GTACTTT20900.035.86842322
TGGTATC56700.035.8095257
AAGCAGT56500.035.478761
AACGCAG57450.035.4647514
ACGCAGA61000.034.0942615
TACATGG27300.033.6575123
GAGTACT23850.033.40251520
Produced by FastQC (version 0.11.5)