##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805271_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1724208
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.153469303007526	33.0	31.0	35.0	25.0	35.0
2	30.99918687304548	35.0	31.0	35.0	17.0	35.0
3	32.8547072046992	35.0	32.0	37.0	18.0	39.0
4	34.122353567551016	37.0	33.0	39.0	27.0	39.0
5	34.46628075035031	37.0	34.0	39.0	27.0	39.0
6	34.26454464890547	37.0	33.0	38.0	27.0	39.0
7	34.57001649452966	37.0	34.0	38.0	27.0	39.0
8	34.837943565973475	37.0	33.0	39.0	27.0	40.0
9	34.55291936935683	37.0	33.0	39.0	27.0	40.0
10	35.03168643226339	37.0	33.0	38.0	27.0	40.0
11	34.36771375611295	36.0	33.0	38.0	27.0	40.0
12	33.91326336497685	36.0	32.0	38.0	26.0	40.0
13	33.849024015664	36.0	32.0	38.0	26.0	39.0
14	33.17206566725128	36.0	32.0	38.0	25.0	39.0
15	32.78629666490354	35.0	31.0	37.0	24.0	39.0
16	32.80610981969693	34.0	31.0	37.0	25.0	39.0
17	31.3078340896226	34.0	29.0	36.0	19.0	38.0
18	31.425239298274917	34.0	29.0	36.0	22.0	38.0
19	30.67315370303351	33.0	27.0	36.0	19.0	38.0
20	29.2423385113629	32.0	27.0	35.0	16.0	37.0
21	28.899537062813767	31.0	26.0	34.0	16.0	37.0
22	27.936118496144317	30.0	24.0	34.0	11.0	36.0
23	27.49262095988419	30.0	24.0	34.0	15.0	36.0
24	26.52318687768529	29.0	24.0	33.0	10.0	35.0
25	26.297089446284904	29.0	24.0	32.0	10.0	35.0
26	25.648992464946225	27.0	19.0	31.0	10.0	34.0
27	24.894388032070378	27.0	19.0	31.0	10.0	34.0
28	24.032802306914245	26.0	17.0	30.0	10.0	34.0
29	23.128632972356005	25.0	16.0	30.0	10.0	33.0
30	22.595203710921187	25.0	16.0	30.0	10.0	33.0
31	22.000854885257464	24.0	15.0	30.0	10.0	32.0
32	21.31173443111272	24.0	15.0	28.0	10.0	31.0
33	20.468828586806232	21.0	13.0	27.0	10.0	30.0
34	19.906251449941074	19.0	10.0	26.0	10.0	30.0
35	19.132438197711647	19.0	10.0	25.0	9.0	30.0
36	18.48292201404935	18.0	10.0	25.0	9.0	30.0
37	18.04731621706894	16.0	10.0	24.0	9.0	30.0
38	17.241240036004935	15.0	10.0	24.0	9.0	28.0
39	16.551515246420387	15.0	10.0	24.0	9.0	27.0
40	16.13067564934161	15.0	10.0	23.0	9.0	26.0
41	15.559372767090746	14.0	10.0	22.0	9.0	25.0
42	15.164471455880033	13.0	10.0	22.0	9.0	24.0
43	14.716165915017214	13.0	10.0	20.0	8.0	24.0
44	14.325121447064392	12.0	9.0	19.0	8.0	24.0
45	13.966239572023794	12.0	9.0	18.0	8.0	24.0
46	13.673376993958966	10.0	9.0	15.0	8.0	23.0
47	13.311094717110697	10.0	9.0	15.0	8.0	23.0
48	13.05229415476555	10.0	9.0	15.0	8.0	23.0
49	12.781606975492515	10.0	9.0	14.0	8.0	23.0
50	12.609268719319246	10.0	9.0	14.0	8.0	22.0
51	12.397028664755064	10.0	9.0	14.0	8.0	22.0
52	12.185473562354426	10.0	9.0	14.0	8.0	22.0
53	11.860392133663687	9.0	9.0	13.0	8.0	21.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
10	4.0
11	33.0
12	118.0
13	493.0
14	1456.0
15	3890.0
16	8544.0
17	17740.0
18	32663.0
19	55537.0
20	86958.0
21	126857.0
22	172812.0
23	224406.0
24	274303.0
25	292465.0
26	225009.0
27	113410.0
28	45309.0
29	19206.0
30	9775.0
31	5865.0
32	3636.0
33	2112.0
34	1071.0
35	411.0
36	114.0
37	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.552023885749284	48.80101472676151	21.11311396304854	17.533847424440673
2	30.374467581637482	38.67422027968783	18.229703144864192	12.72160899381049
3	30.528045340237377	25.559967242931247	25.578410493397545	18.33357692343383
4	22.83894982507911	28.597535796145245	21.99949194064753	26.564022438128116
5	19.66502881322903	32.246341508681084	25.880114232157602	22.208515445932278
6	24.17805740374711	25.350189768287816	26.368048402512922	24.10370442545215
7	22.976926217718514	25.749387544890173	28.20622569898759	23.06746053840372
8	21.680736894852593	26.28621372827408	29.47846199530451	22.554587381568815
9	24.231995211714597	25.043208244016963	28.40944943997476	22.315347104293682
10	22.59802761615768	26.550682980243685	29.0789162328443	21.772373170754342
11	21.880016796117406	29.211034863543144	26.673637983352354	22.235310356987092
12	21.92096313205831	25.746545660384363	29.446621289310805	22.885869918246524
13	21.99978192886241	26.978067611332275	26.720848064734646	24.301302395070664
14	22.21245928565463	28.787072093390126	26.26017278657795	22.740295834377292
15	21.91371342668634	28.75146154060299	26.45748076798159	22.877344264729082
16	22.613802975047097	26.260288781863906	26.30929679017845	24.816611452910553
17	24.14088091459963	26.042739623061717	27.399130499336504	22.41724896300214
18	22.283506398299973	25.731234282638756	26.39768519807355	25.58757412098772
19	22.34138804598981	28.71161715987862	26.40296298358435	22.544031810547217
20	24.744288392119746	26.220502398782514	26.91670610506389	22.118503104033852
21	22.641873834247374	28.40301170160445	26.093719551237438	22.86139491291074
22	24.484748939803087	26.07313038798103	27.034267327375815	22.407853344840063
23	22.05105184525301	26.18361589784991	29.538779543999333	22.22655271289775
24	22.523964626077596	28.313057357349	26.218240490706457	22.944737525866948
25	22.17099097092694	26.3922914172768	26.208090903185692	25.228626708610562
26	22.214083219657955	27.62729322680326	27.647244415986933	22.511379137551852
27	22.707759156667873	25.70455536686989	28.82100071453096	22.766684761931273
28	23.85356059129757	26.040941696129472	27.463565880682612	22.641931831890354
29	23.184847767786717	25.922800497387787	28.186506500375824	22.705845234449672
30	24.198124588216736	25.647021705037908	27.406902183495262	22.747951523250094
31	22.995079479969935	26.61175449829719	27.625321306942084	22.76784471479079
32	22.681138238541987	26.482071768603323	27.879176990247117	22.957613002607573
33	22.62076269220419	25.966878706049386	27.996564219630116	23.41579438211631
34	22.649181537262326	26.676189879643292	27.706749997680095	22.96787858541429
35	22.178704657442722	26.15716897265295	28.32262696844	23.341499401464326
36	22.35710540723625	26.0102029453523	28.212431446786002	23.42026020062545
37	22.286812263949592	26.39426333713798	28.209821552852095	23.10910284606034
38	21.92635691285506	26.121500422222844	28.596317845642755	23.355824819279345
39	21.99021231777141	26.84838488163841	27.969305327431492	23.19209747315869
40	21.904491801453187	26.63106771340813	28.26938513218823	23.195055352950455
41	22.013469372604696	26.218008500134555	28.48670230041851	23.281819826842238
42	22.128884682126518	25.971112533986616	28.40301170160445	23.496991082282417
43	21.82677495986563	26.424886092629198	28.453005669849578	23.295333277655597
44	22.073322940155713	26.234131844881826	28.431372549019606	23.261172665942855
45	21.856875736570068	25.942287705427653	28.632276384287742	23.56856017371454
46	22.07320694486976	26.298161242727097	28.188826406094858	23.439805406308288
47	21.899446006514296	26.19480944294424	28.24786800664421	23.657876543897256
48	22.1238968848306	26.013160825144066	28.18447658287167	23.678465707153663
49	22.20712350250086	26.394379332423927	27.995926245557378	23.40257091951783
50	22.05627163312083	26.241961526683554	28.021909189610533	23.679857650585078
51	22.167105128847563	26.22375026678916	27.9496441264627	23.65950047790058
52	22.334544324118667	26.354592949342535	27.813349665469595	23.4975130610692
53	22.32184284030697	26.324608167924058	27.612561825487415	23.740987166281563
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	584.0
1	621.5
2	659.0
3	684.0
4	709.0
5	710.5
6	712.0
7	690.0
8	652.0
9	636.0
10	644.0
11	652.0
12	760.5
13	869.0
14	985.0
15	1101.0
16	1400.0
17	1699.0
18	2239.0
19	2779.0
20	3974.5
21	5170.0
22	8682.5
23	12195.0
24	13703.5
25	17900.5
26	20589.0
27	23935.5
28	27282.0
29	31518.5
30	35755.0
31	40706.5
32	45658.0
33	51710.0
34	57762.0
35	64248.0
36	70734.0
37	77571.5
38	84409.0
39	91770.0
40	99131.0
41	106972.5
42	121246.5
43	127679.0
44	133475.0
45	139271.0
46	141770.5
47	144270.0
48	143847.5
49	143425.0
50	138851.5
51	134278.0
52	126474.5
53	118671.0
54	108463.5
55	98256.0
56	86373.5
57	74491.0
58	64106.5
59	44743.5
60	35765.0
61	29398.0
62	23031.0
63	18423.5
64	13816.0
65	10924.0
66	8032.0
67	6257.5
68	4483.0
69	3462.5
70	2442.0
71	1860.5
72	1279.0
73	977.0
74	675.0
75	516.0
76	286.5
77	216.0
78	181.0
79	146.0
80	112.5
81	79.0
82	52.5
83	26.0
84	18.5
85	11.0
86	7.5
87	4.0
88	3.5
89	3.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1724208.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.35862390280928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.14339383929824	91.66316963924824
2	1.8326413810375042	3.4585103764408576
3	0.4924779398511316	1.3940862212052976
4	0.2030255626556121	0.7662885083710858
5	0.11671388904031413	0.5506480980094608
6	0.0618655607089094	0.35025295052810385
7	0.04260287388816278	0.2813963988074376
8	0.02550207784707591	0.192507277784992
9	0.01758757861415638	0.14935857442328462
>10	0.06300337241557262	0.9074510373132422
>50	6.941194284145125E-4	0.04250463547128943
>100	4.303418298925234E-4	0.0741879699661783
>500	0.0	0.0
>1k	6.146338495725565E-5	0.1696383124305267
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2925	0.16964310570418417	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	5.799764297578947E-5	0.0	0.0	0.0	0.0
5	5.799764297578947E-5	0.0	0.0	5.799764297578947E-5	0.0
6	5.799764297578947E-5	0.0	0.0	5.799764297578947E-5	0.0
7	5.799764297578947E-5	0.0	0.0	5.799764297578947E-5	0.0
8	5.799764297578947E-5	0.0	0.0	5.799764297578947E-5	0.0
9	5.799764297578947E-5	0.0	0.0	5.799764297578947E-5	0.0
10	5.799764297578947E-5	0.0	0.0	1.1599528595157893E-4	0.0
11	5.799764297578947E-5	0.0	0.0	1.1599528595157893E-4	0.0
12	1.1599528595157893E-4	0.0	0.0	1.1599528595157893E-4	0.0
13	1.1599528595157893E-4	0.0	0.0	1.1599528595157893E-4	0.0
14	1.1599528595157893E-4	0.0	0.0	1.739929289273684E-4	0.0
15	1.1599528595157893E-4	0.0	0.0	1.739929289273684E-4	0.0
16	1.1599528595157893E-4	0.0	0.0	2.3199057190315787E-4	0.0
17	1.1599528595157893E-4	0.0	0.0	2.3199057190315787E-4	0.0
18	1.1599528595157893E-4	0.0	0.0	2.3199057190315787E-4	0.0
19	1.1599528595157893E-4	0.0	0.0	2.3199057190315787E-4	0.0
20	2.3199057190315787E-4	0.0	0.0	2.3199057190315787E-4	0.0
21	2.3199057190315787E-4	0.0	0.0	2.3199057190315787E-4	0.0
22	2.3199057190315787E-4	0.0	0.0	2.3199057190315787E-4	0.0
23	2.3199057190315787E-4	0.0	0.0	2.3199057190315787E-4	0.0
24	2.3199057190315787E-4	0.0	0.0	2.3199057190315787E-4	0.0
25	2.3199057190315787E-4	0.0	0.0	2.899882148789473E-4	0.0
26	2.3199057190315787E-4	0.0	0.0	2.899882148789473E-4	0.0
27	2.899882148789473E-4	0.0	0.0	2.899882148789473E-4	0.0
28	3.479858578547368E-4	0.0	0.0	2.899882148789473E-4	0.0
29	4.0598350083052625E-4	0.0	0.0	3.479858578547368E-4	0.0
30	4.6398114380631573E-4	0.0	0.0	3.479858578547368E-4	0.0
31	4.6398114380631573E-4	0.0	0.0	4.0598350083052625E-4	0.0
32	4.6398114380631573E-4	0.0	0.0	4.0598350083052625E-4	0.0
33	4.6398114380631573E-4	0.0	0.0	4.0598350083052625E-4	0.0
34	4.6398114380631573E-4	0.0	0.0	4.0598350083052625E-4	0.0
35	4.6398114380631573E-4	0.0	0.0	4.0598350083052625E-4	0.0
36	4.6398114380631573E-4	0.0	0.0	4.0598350083052625E-4	0.0
37	5.219787867821052E-4	0.0	0.0	5.219787867821052E-4	0.0
38	6.379740727336841E-4	0.0	0.0	5.799764297578946E-4	0.0
39	6.959717157094735E-4	0.0	0.0	5.799764297578946E-4	0.0
40	7.539693586852631E-4	0.0	0.0	6.379740727336841E-4	0.0
41	7.539693586852631E-4	0.0	0.0	6.379740727336841E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATGGG	2340	0.0	37.76068	24
GTATCAA	5415	0.0	37.58264	9
AGTGGTA	5375	0.0	37.293957	5
CAACGCA	5760	0.0	36.881943	13
GTGGTAT	5445	0.0	36.814507	6
TCAACGC	5765	0.0	36.64614	12
ATCAACG	5700	0.0	36.610527	11
AGAGTAC	5505	0.0	36.541325	19
GGTATCA	5480	0.0	36.40785	8
CATGGGG	1275	0.0	36.125492	25
GCAGTGG	5295	0.0	36.03777	3
TATCAAC	6025	0.0	36.000828	10
CAGTGGT	5595	0.0	35.99553	4
GTACTTT	2090	0.0	35.868423	22
TGGTATC	5670	0.0	35.809525	7
AAGCAGT	5650	0.0	35.47876	1
AACGCAG	5745	0.0	35.46475	14
ACGCAGA	6100	0.0	34.09426	15
TACATGG	2730	0.0	33.65751	23
GAGTACT	2385	0.0	33.402515	20
>>END_MODULE