FastQCFastQC Report
Thu 26 May 2016
SRR805270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25879901
Sequences flagged as poor quality0
Sequence length53
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT328550.12695179939057727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC256300.033.71420312
CAACGCA265250.032.5323313
ATCAACG267300.032.37074311
GTATCAA271550.031.8035359
AACGCAG272650.031.58903514
GGTATCA276700.031.2710518
AGTGGTA282850.030.4831185
TATCAAC297050.029.19996610
GTGGTAT295150.029.1809256
AAGCAGT295850.029.159541
ACGCAGA297350.029.02824815
AGAGTAC291050.028.3970119
TGGTATC306550.028.2797267
CGCAGAG317200.027.21910316
GCAGTGG343800.025.270363
CAGTGGT352250.024.7775734
AGCAGTG360950.023.8938922
CAGAGTA358150.023.58201818
ACATGGG206400.023.32921424
GCAGAGT382650.022.46517817
Produced by FastQC (version 0.11.5)