##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805270_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25879901
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.279509569994104	35.0	35.0	37.0	32.0	37.0
2	34.63908895942067	36.0	35.0	37.0	32.0	37.0
3	36.6056931979763	39.0	37.0	39.0	32.0	39.0
4	36.64223781226984	39.0	37.0	39.0	32.0	39.0
5	36.62465281455288	39.0	37.0	39.0	32.0	39.0
6	36.62667902013999	38.0	37.0	39.0	32.0	39.0
7	36.58318213813878	38.0	37.0	39.0	32.0	39.0
8	37.4236666902242	40.0	37.0	40.0	32.0	40.0
9	37.346609247075556	39.0	37.0	40.0	32.0	40.0
10	37.14502393189217	39.0	36.0	40.0	32.0	40.0
11	36.828628788031295	38.0	36.0	40.0	32.0	40.0
12	36.6057761967482	38.0	36.0	40.0	31.0	40.0
13	36.58632701106546	38.0	36.0	40.0	31.0	40.0
14	36.24548853567871	38.0	35.0	40.0	30.0	40.0
15	36.06413015258443	38.0	35.0	40.0	30.0	40.0
16	35.85491629199045	38.0	34.0	40.0	30.0	40.0
17	35.54232603130901	37.0	34.0	39.0	29.0	40.0
18	35.2085577916237	37.0	34.0	39.0	28.0	40.0
19	34.97286631815168	37.0	33.0	39.0	27.0	40.0
20	34.52218847359578	36.0	33.0	39.0	27.0	40.0
21	34.13890663646666	36.0	32.0	38.0	27.0	40.0
22	33.75661232243508	36.0	32.0	38.0	26.0	40.0
23	33.40692168799254	36.0	31.0	38.0	25.0	40.0
24	32.683204159088554	34.0	30.0	38.0	24.0	39.0
25	32.40401360113395	34.0	30.0	38.0	24.0	39.0
26	32.093110557107615	34.0	30.0	38.0	24.0	39.0
27	31.593980402011585	34.0	29.0	37.0	22.0	39.0
28	31.208883565667428	33.0	29.0	37.0	19.0	38.0
29	30.826678626011745	33.0	27.0	37.0	19.0	38.0
30	30.313822413771984	32.0	27.0	36.0	18.0	38.0
31	29.845265057234958	32.0	26.0	36.0	16.0	38.0
32	29.418516013643174	31.0	25.0	36.0	16.0	38.0
33	29.004909601470267	31.0	25.0	35.0	16.0	38.0
34	28.364536518126556	30.0	24.0	35.0	15.0	38.0
35	27.90680219371782	30.0	24.0	35.0	15.0	38.0
36	27.412143732698205	30.0	23.0	34.0	10.0	37.0
37	26.928127391213746	30.0	22.0	34.0	10.0	37.0
38	26.422997251805562	29.0	19.0	34.0	10.0	37.0
39	25.941088723639243	28.0	19.0	33.0	10.0	37.0
40	25.485617893206005	27.0	18.0	33.0	10.0	36.0
41	24.83213533158415	27.0	18.0	33.0	10.0	36.0
42	24.39000979949653	26.0	15.0	32.0	10.0	35.0
43	23.90834825836467	25.0	15.0	32.0	10.0	35.0
44	23.382722947819623	24.0	15.0	31.0	9.0	35.0
45	22.951182618511563	24.0	14.0	31.0	9.0	35.0
46	22.514940377863116	24.0	14.0	31.0	9.0	34.0
47	21.916410460766446	23.0	13.0	30.0	9.0	34.0
48	21.51826369042138	23.0	12.0	30.0	9.0	33.0
49	21.134930848460357	23.0	12.0	29.0	9.0	33.0
50	20.739687296330846	22.0	10.0	29.0	9.0	33.0
51	20.325013917170704	22.0	10.0	28.0	9.0	33.0
52	19.922160289562157	21.0	10.0	28.0	9.0	32.0
53	18.979298954814393	19.0	9.0	27.0	9.0	31.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	3.0
9	8.0
10	27.0
11	156.0
12	626.0
13	1766.0
14	4299.0
15	9484.0
16	19783.0
17	40182.0
18	78447.0
19	143126.0
20	237916.0
21	361976.0
22	511280.0
23	675401.0
24	863310.0
25	1085484.0
26	1414149.0
27	2054568.0
28	3025938.0
29	2910721.0
30	1675840.0
31	1026616.0
32	971073.0
33	1218424.0
34	1987546.0
35	3084970.0
36	1920544.0
37	458559.0
38	92109.0
39	5569.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.844905782290283	31.310792108516956	19.412025571504312	26.432276537688455
2	26.504738175003066	25.11653348287538	28.282001542432482	20.096726799689073
3	21.918974883250133	25.091912059478126	27.205548429261768	25.783564628009977
4	22.050613717571792	28.350981713569922	26.157453229824952	23.44095133903333
5	24.832239505089294	27.611148898908077	25.432013824164162	22.12459777183846
6	24.44635703977384	25.357001945254737	27.1390837236974	23.057557291274026
7	24.010609623274835	26.230347635410194	26.744012660635757	23.01503008067921
8	23.115803263698727	25.299215789117586	27.49800318015127	24.086977767032415
9	23.436279760111912	25.017835269153466	27.804816564020086	23.74106840671454
10	23.306553606986363	25.798900853600635	27.526326317863425	23.368219221549573
11	23.204741007316837	26.612980474693472	26.91158671742987	23.270691800559824
12	23.175370724949836	25.688061944286417	27.426689151554328	23.70987817920942
13	23.4904685299994	25.93601497934633	26.407342130095472	24.1661743605588
14	23.457933629653375	26.62721545959546	26.520259872709712	23.39459103804145
15	23.40480359642798	26.433188442258725	26.468748856496788	23.693259104816512
16	23.7923282627704	25.86573263939456	26.12622436229567	24.21571473553937
17	24.47818869168008	25.793263274075123	26.23486465423496	23.493683380009838
18	23.549197502726148	25.629754147823054	26.20368215473467	24.617366194716123
19	23.940087715173252	26.334161788331418	26.00580659099121	23.71994390550412
20	24.483134614773064	25.831899434236632	26.12397164888691	23.560994302103396
21	23.75884668183236	26.319582907214368	26.138608490040205	23.782961920913067
22	24.544143348925484	25.75921368478187	25.877115990513254	23.81952697577939
23	23.71163630030888	25.80646270632952	26.99996804470002	23.481932948661587
24	23.699213532540174	26.2862829343899	26.206348316401986	23.808155216667945
25	23.762834332326076	25.834712427995765	25.906845625104978	24.495607614573178
26	23.778004405812837	26.225108048133567	26.450839205296806	23.54604834075679
27	23.72335195563538	25.51333175501715	26.904971545293005	23.85834474405447
28	24.310487122806226	25.62202614299027	26.179304163489654	23.88818257071385
29	24.196286531389745	25.78842167904738	26.44799916352076	23.567292626042118
30	24.21022012410326	25.49704498483205	26.388706046441214	23.90402884462348
31	24.161808037828276	25.785214557041776	26.167495772105156	23.885481633024792
32	23.97505307303919	25.935273090882383	26.374331957452235	23.715341878626198
33	23.87363846561855	25.654255787145395	26.391225376016703	24.080880371219347
34	23.96729802018949	25.86038099604786	26.195513653626417	23.976807330136232
35	23.909871216277065	25.830713185494798	26.454185431389398	23.805230166838736
36	23.707683425836905	25.64947215215391	26.52060763292719	24.122236789082
37	23.908870439651217	25.82782291168734	26.28004643448984	23.983260214171608
38	23.808499112883005	25.812281893968603	26.529208902306078	23.85001009084231
39	23.753166598280266	25.554807956954704	26.540457013340195	24.151568431424835
40	23.8416638456229	25.646972142590503	26.419023009400227	24.09234100238637
41	23.691593719775046	25.73625764642608	26.716195707240146	23.85595292655872
42	23.5679726904674	25.538579146805855	26.664541722937813	24.22890643978893
43	23.733116289741606	25.675944432708608	26.444084929072954	24.146854348476836
44	23.704727463988366	25.609642015245733	26.838518431735885	23.847112089030016
45	23.389915595117618	25.58947578663458	26.776257760800554	24.24435085744725
46	23.60548442592574	25.659190118231134	26.62868378051369	24.10664167532944
47	23.448486143745296	25.768464106566714	26.81891248347511	23.96413726621288
48	23.31275146686226	25.614607258350798	26.907324722764585	24.165316552022357
49	23.534467925514864	25.6542789711599	26.606145054418874	24.20510804890637
50	23.23044048738826	25.761899166461262	26.935246004225444	24.072414341925032
51	23.33916965138313	25.45489258247163	26.910744364903096	24.295193401242145
52	23.307202759392318	25.70203031302168	26.697509391554476	24.293257536031533
53	23.108040482844196	25.908163249928972	26.942278488623277	24.04151777860356
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16216.0
1	11267.0
2	6318.0
3	5783.0
4	5248.0
5	5172.0
6	5096.0
7	5266.0
8	5568.0
9	5700.0
10	6506.0
11	7312.0
12	8563.0
13	9814.0
14	11624.5
15	13435.0
16	17561.5
17	21688.0
18	29232.0
19	36776.0
20	49232.5
21	61689.0
22	99056.5
23	136424.0
24	147747.0
25	190393.5
26	221717.0
27	262408.5
28	303100.0
29	351032.5
30	398965.0
31	458084.5
32	517204.0
33	592300.5
34	667397.0
35	746144.0
36	824891.0
37	922401.0
38	1019911.0
39	1132879.5
40	1245848.0
41	1354046.0
42	1573144.0
43	1684044.0
44	1770332.0
45	1856620.0
46	1920267.5
47	1983915.0
48	2042690.5
49	2101466.0
50	2113427.0
51	2125388.0
52	2084944.0
53	2044500.0
54	1939456.0
55	1834412.0
56	1671106.0
57	1507800.0
58	1347103.5
59	1027679.5
60	868952.0
61	733101.5
62	597251.0
63	489898.0
64	382545.0
65	309319.5
66	236094.0
67	188501.0
68	140908.0
69	111144.0
70	81380.0
71	63780.5
72	46181.0
73	35557.0
74	24933.0
75	18884.0
76	9605.5
77	6376.0
78	4704.5
79	3033.0
80	2484.0
81	1935.0
82	1422.0
83	909.0
84	598.5
85	288.0
86	200.0
87	112.0
88	87.5
89	63.0
90	48.5
91	34.0
92	22.5
93	9.0
94	7.0
95	4.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.5879901E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.13123899794996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.30700146940245	58.39851419365372
2	6.122393803725378	8.097629757474618
3	1.9772815539943815	3.92280237040321
4	0.9898695558793793	2.618452007066153
5	0.5752661420537025	1.902153136379102
6	0.37082440139868744	1.4713846269100999
7	0.2792014771856134	1.292475773143973
8	0.1952704955910306	1.0330783850542864
9	0.15120425397380008	0.8999392191343288
>10	0.9050241959795013	11.31013941329516
>50	0.0830134555780404	3.7926860747461646
>100	0.043118581587401415	4.861264653567223
>500	4.8373327282163174E-4	0.19395736042744408
>1k	2.9355541529048453E-5	0.026918920224649704
>5k	1.1683224072806235E-5	0.051680958715888736
>10k+	5.841612034065773E-6	0.12692314980391517
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32855	0.12695179939057727	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.114405074424357E-5	0.0	0.0	0.0	3.864002416392551E-6
2	8.114405074424357E-5	0.0	0.0	0.0	3.864002416392551E-6
3	8.114405074424357E-5	0.0	0.0	0.0	3.864002416392551E-6
4	8.114405074424357E-5	0.0	0.0	0.0	3.864002416392551E-6
5	8.114405074424357E-5	0.0	0.0	0.0	3.864002416392551E-6
6	8.114405074424357E-5	0.0	0.0	0.0	3.864002416392551E-6
7	8.114405074424357E-5	0.0	0.0	0.0	7.728004832785101E-6
8	8.114405074424357E-5	7.728004832785101E-6	0.0	0.0	1.1592007249177653E-5
9	8.114405074424357E-5	1.1592007249177653E-5	0.0	0.0	1.5456009665570203E-5
10	8.114405074424357E-5	1.5456009665570203E-5	0.0	0.0	1.5456009665570203E-5
11	8.500805316063613E-5	1.5456009665570203E-5	0.0	3.864002416392551E-6	1.5456009665570203E-5
12	8.500805316063613E-5	1.9320012081962754E-5	0.0	3.864002416392551E-6	1.5456009665570203E-5
13	8.500805316063613E-5	1.9320012081962754E-5	0.0	3.864002416392551E-6	1.5456009665570203E-5
14	9.273605799342122E-5	1.9320012081962754E-5	0.0	7.728004832785101E-6	1.5456009665570203E-5
15	1.0046406282620633E-4	2.3184014498355306E-5	0.0	7.728004832785101E-6	1.5456009665570203E-5
16	1.1205607007538398E-4	2.7048016914747857E-5	0.0	1.1592007249177653E-5	1.5456009665570203E-5
17	1.1205607007538398E-4	2.7048016914747857E-5	0.0	1.1592007249177653E-5	1.5456009665570203E-5
18	1.275120797409542E-4	2.7048016914747857E-5	0.0	1.1592007249177653E-5	1.5456009665570203E-5
19	1.3910408699013184E-4	2.7048016914747857E-5	0.0	1.1592007249177653E-5	1.5456009665570203E-5
20	1.584240990720946E-4	2.7048016914747857E-5	0.0	1.1592007249177653E-5	1.5456009665570203E-5
21	2.1252013290159032E-4	2.7048016914747857E-5	0.0	1.1592007249177653E-5	1.9320012081962754E-5
22	2.4343215223273073E-4	2.7048016914747857E-5	0.0	1.1592007249177653E-5	1.9320012081962754E-5
23	2.782081739802637E-4	2.7048016914747857E-5	3.864002416392551E-6	1.5456009665570203E-5	2.3184014498355306E-5
24	3.0912019331140407E-4	2.7048016914747857E-5	3.864002416392551E-6	1.5456009665570203E-5	2.3184014498355306E-5
25	3.477602174753296E-4	2.7048016914747857E-5	3.864002416392551E-6	1.5456009665570203E-5	2.3184014498355306E-5
26	3.902642440556477E-4	2.7048016914747857E-5	3.864002416392551E-6	1.9320012081962754E-5	2.7048016914747857E-5
27	4.4822428030153594E-4	2.7048016914747857E-5	3.864002416392551E-6	1.9320012081962754E-5	2.7048016914747857E-5
28	4.984563117146391E-4	3.0912019331140406E-5	3.864002416392551E-6	2.3184014498355306E-5	2.7048016914747857E-5
29	5.602803503769199E-4	3.0912019331140406E-5	3.864002416392551E-6	2.3184014498355306E-5	2.7048016914747857E-5
30	6.259683914555933E-4	3.0912019331140406E-5	3.864002416392551E-6	3.0912019331140406E-5	2.7048016914747857E-5
31	7.187044494490145E-4	3.0912019331140406E-5	3.864002416392551E-6	3.864002416392551E-5	2.7048016914747857E-5
32	8.26896517108006E-4	3.0912019331140406E-5	7.728004832785101E-6	4.2504026580318064E-5	3.0912019331140406E-5
33	9.273605799342123E-4	3.0912019331140406E-5	7.728004832785101E-6	4.2504026580318064E-5	3.477602174753296E-5
34	0.0010432806524259888	3.0912019331140406E-5	7.728004832785101E-6	7.341604591145848E-5	3.477602174753296E-5
35	0.0011398807128358026	3.0912019331140406E-5	7.728004832785101E-6	8.500805316063613E-5	4.2504026580318064E-5
36	0.0012712567949931493	3.0912019331140406E-5	7.728004832785101E-6	1.0432806524259887E-4	4.2504026580318064E-5
37	0.001429680894065244	3.0912019331140406E-5	7.728004832785101E-6	1.0819206765899143E-4	4.2504026580318064E-5
38	0.0016074250052193013	3.0912019331140406E-5	7.728004832785101E-6	1.1978407490816908E-4	4.2504026580318064E-5
39	0.0017967611236225363	3.477602174753296E-5	7.728004832785101E-6	1.275120797409542E-4	4.2504026580318064E-5
40	0.002005417254107734	3.477602174753296E-5	7.728004832785101E-6	1.4296808940652438E-4	4.2504026580318064E-5
41	0.0021986173749273617	3.477602174753296E-5	7.728004832785101E-6	1.7774411115405736E-4	4.2504026580318064E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	25630	0.0	33.714203	12
CAACGCA	26525	0.0	32.53233	13
ATCAACG	26730	0.0	32.370743	11
GTATCAA	27155	0.0	31.803535	9
AACGCAG	27265	0.0	31.589035	14
GGTATCA	27670	0.0	31.271051	8
AGTGGTA	28285	0.0	30.483118	5
TATCAAC	29705	0.0	29.199966	10
GTGGTAT	29515	0.0	29.180925	6
AAGCAGT	29585	0.0	29.15954	1
ACGCAGA	29735	0.0	29.028248	15
AGAGTAC	29105	0.0	28.39701	19
TGGTATC	30655	0.0	28.279726	7
CGCAGAG	31720	0.0	27.219103	16
GCAGTGG	34380	0.0	25.27036	3
CAGTGGT	35225	0.0	24.777573	4
AGCAGTG	36095	0.0	23.893892	2
CAGAGTA	35815	0.0	23.582018	18
ACATGGG	20640	0.0	23.329214	24
GCAGAGT	38265	0.0	22.465178	17
>>END_MODULE