FastQCFastQC Report
Thu 26 May 2016
SRR805269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31248149
Sequences flagged as poor quality0
Sequence length53
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1001110.32037417640321675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC569950.041.2358112
CAACGCA579800.040.51500713
ATCAACG582500.040.36351811
AACGCAG586800.039.9996614
GGTATCA592500.039.7893688
GTATCAA592900.039.718929
AGTGGTA600450.039.059045
GTGGTAT614850.038.2398156
ACGCAGA614450.038.20351815
TATCAAC619950.038.01621210
AAGCAGT619950.037.9820981
AGAGTAC603150.037.8672819
TGGTATC625150.037.6511237
CGCAGAG637400.036.8648416
GCAGTGG671650.035.093433
CAGTGGT687350.034.2679144
CAGAGTA678350.034.0262418
AGCAGTG696100.033.8135342
ACATGGG386950.033.2079124
GCAGAGT706600.033.16473417
Produced by FastQC (version 0.11.5)