##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805269_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31248149
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.73497627011443	35.0	35.0	37.0	32.0	37.0
2	35.120407900000735	37.0	35.0	37.0	33.0	37.0
3	37.079801590807826	39.0	37.0	39.0	33.0	39.0
4	37.04986084135736	39.0	37.0	39.0	33.0	39.0
5	36.97442690125421	39.0	37.0	39.0	33.0	39.0
6	36.979596007430715	39.0	37.0	39.0	33.0	39.0
7	36.90582722195801	39.0	37.0	39.0	33.0	39.0
8	37.79016200287575	40.0	38.0	40.0	33.0	40.0
9	37.70752222795661	40.0	37.0	40.0	33.0	40.0
10	37.5017575921057	39.0	37.0	40.0	33.0	40.0
11	37.210427664051394	39.0	36.0	40.0	32.0	40.0
12	37.01413354115791	38.0	36.0	40.0	32.0	40.0
13	36.982362603301716	38.0	36.0	40.0	32.0	40.0
14	36.67759504730984	38.0	36.0	40.0	31.0	40.0
15	36.48748253856572	38.0	36.0	40.0	31.0	40.0
16	36.27666272328643	38.0	35.0	40.0	31.0	40.0
17	35.96354881692353	38.0	34.0	40.0	30.0	40.0
18	35.63441338557365	38.0	34.0	39.0	29.0	40.0
19	35.418167040870166	37.0	34.0	39.0	29.0	40.0
20	34.99351062362126	37.0	33.0	39.0	28.0	40.0
21	34.569296024542126	36.0	33.0	39.0	27.0	40.0
22	34.25253604621509	36.0	32.0	38.0	27.0	40.0
23	33.890969925930655	36.0	32.0	38.0	27.0	40.0
24	33.21938406655703	35.0	31.0	38.0	25.0	40.0
25	32.9664468445795	35.0	31.0	38.0	24.0	39.0
26	32.633466225471466	34.0	30.0	38.0	24.0	39.0
27	32.15110437421429	34.0	30.0	38.0	24.0	39.0
28	31.756482984000108	34.0	29.0	37.0	23.0	39.0
29	31.36826520508463	33.0	29.0	37.0	21.0	38.0
30	30.88346423335347	33.0	27.0	37.0	19.0	38.0
31	30.44595387073967	33.0	27.0	36.0	18.0	38.0
32	30.01540008017755	32.0	26.0	36.0	18.0	38.0
33	29.581182872623913	31.0	25.0	36.0	16.0	38.0
34	28.967951573707612	31.0	25.0	35.0	15.0	38.0
35	28.5239623953406	30.0	24.0	35.0	15.0	38.0
36	28.03927458231206	30.0	24.0	35.0	15.0	38.0
37	27.543251569876986	30.0	24.0	34.0	13.0	37.0
38	27.052589098957508	30.0	23.0	34.0	10.0	37.0
39	26.552428369437177	29.0	20.0	34.0	10.0	37.0
40	26.0926377431188	29.0	19.0	33.0	10.0	37.0
41	25.45167852982268	27.0	19.0	33.0	10.0	36.0
42	25.00685221387033	27.0	18.0	33.0	10.0	36.0
43	24.510888020919253	26.0	16.0	32.0	10.0	35.0
44	23.963877668402056	26.0	15.0	32.0	10.0	35.0
45	23.547053811091338	24.0	15.0	31.0	9.0	35.0
46	23.081983800064446	24.0	14.0	31.0	9.0	35.0
47	22.491473846978906	24.0	13.0	31.0	9.0	34.0
48	22.074053218320227	23.0	13.0	30.0	9.0	34.0
49	21.718604772397878	23.0	13.0	30.0	9.0	33.0
50	21.29167097225503	23.0	12.0	29.0	9.0	33.0
51	20.87157712925652	22.0	11.0	29.0	9.0	33.0
52	20.462199088976437	22.0	10.0	28.0	9.0	33.0
53	19.522147375833367	19.0	10.0	28.0	9.0	32.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	35.0
11	95.0
12	431.0
13	1222.0
14	3064.0
15	6958.0
16	15371.0
17	32879.0
18	67312.0
19	126683.0
20	216705.0
21	336845.0
22	483565.0
23	649229.0
24	841908.0
25	1085725.0
26	1459208.0
27	2255507.0
28	3616772.0
29	3702057.0
30	2142739.0
31	1252132.0
32	1150359.0
33	1460322.0
34	2479525.0
35	4169288.0
36	2834952.0
37	713551.0
38	135797.0
39	7905.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.343872240240533	35.92831050568787	17.937513674809985	24.790303579261607
2	28.002045817177844	28.32836914596125	26.9143845928282	16.7552004440327
3	23.69614276992855	24.549630123691486	27.264882153499713	24.48934495288025
4	21.631642885471393	28.536282261070888	25.633351914700615	24.198722938757108
5	22.965033864885882	28.657457438518996	25.837879229262505	22.53962946733261
6	24.257286407588495	25.013407994182312	27.79893298639865	22.930372611830542
7	23.270978386591796	25.886333299293984	27.57423807726979	23.26845023684443
8	23.172684564452123	25.071984263771913	27.913790989667902	23.84154018210807
9	24.068433621460265	24.87999529188113	27.77320026219793	23.278370824460676
10	22.956422154797075	25.87278049653437	28.343387635536427	22.827409713132127
11	22.86132852221103	27.51791474112595	26.898518053021313	22.72223868364171
12	22.83459413868002	25.583118539277315	28.35482511300109	23.227462209041565
13	23.120595079087725	25.946919287923265	26.418675230971285	24.513810402017732
14	23.10357647104153	27.462292246494346	26.516537027521213	22.91759425494291
15	23.055660032855066	27.2074803534763	26.42239705142215	23.314462562246486
16	23.48310935153311	25.76343962005557	26.045766742855715	24.707684285555604
17	24.982238147929976	25.7122525881453	26.186197460848	23.11931180307672
18	23.236736358367978	25.48961860108898	26.166580298884263	25.10706474165878
19	23.616179633552054	27.184486991533486	25.893578528443395	23.305754846471068
20	25.08390817004873	25.784909691770864	26.030034611010077	23.10114752717033
21	23.479445134494206	27.08886532767109	26.06106364892205	23.370625888912652
22	25.081268013666985	25.73306022062299	25.791374074669193	23.394297691040837
23	23.37036667355881	25.784519268645322	27.764114924055182	23.08099913374069
24	23.42060644936121	27.007330898223763	26.09795543409627	23.474107218318753
25	23.423733034555102	25.782627956619127	25.807029401965536	24.986609606860235
26	23.447404836683287	26.65072097550482	26.76107951226167	23.14079467555022
27	23.42680841671614	25.397718757677456	27.677389787151874	23.498083038454535
28	24.505768325669468	25.60519984719735	26.358364458643614	23.53066736848957
29	24.391950384005145	25.740318250530613	26.701440779740267	23.166290585723974
30	24.41821753986132	25.42148016511314	26.6383074402263	23.52199485479924
31	24.124228926327763	25.891933631012833	26.38820622623119	23.595631216428213
32	23.82863061744873	26.055376912085254	26.700512724769716	23.415479745696295
33	23.764991647985294	25.68861918829176	26.70326488778583	23.84312427593711
34	23.78244548181078	26.032197939148332	26.488698578594207	23.696658000446682
35	23.73556910522924	25.952382011491302	26.780517463610405	23.53153141966905
36	23.57399793504569	25.653708320451234	26.865808915593686	23.906484828909385
37	23.715849537199787	25.92045052012521	26.631391830600908	23.732308112074094
38	23.67553034901363	25.89954368177136	26.840319405799047	23.584606563415964
39	23.65144572243303	25.60135321935389	26.843756409379637	23.90344464883344
40	23.681796320159634	25.79149888206178	26.665550653896332	23.861154143882253
41	23.58096154751438	25.839082500534673	26.98989306534605	23.590062886604898
42	23.450912244434065	25.62030794208002	26.961862605045823	23.96691720844009
43	23.536485313098066	25.854485012856284	26.721409962554905	23.88761971149075
44	23.61106893083491	25.72681025042475	27.09781945804214	23.5643013606982
45	23.257892811507013	25.685345394378402	27.06561275037443	23.991149043740158
46	23.44103645947157	25.78219273083983	26.879540928968304	23.897229880720296
47	23.268591045184788	25.89471779592449	27.09011980197611	23.74657135691461
48	23.16563454686548	25.678452186079888	27.21814658525854	23.937766681796095
49	23.41850072463492	25.832602116688573	26.80278118233499	23.946115976341513
50	23.084240925758515	25.89825080519169	27.163880970997674	23.853627298052118
51	23.194372249057057	25.569536934811723	27.164825026915995	24.071265789215225
52	23.179977796444838	25.849451114688428	26.88917989990383	24.081391188962904
53	22.999675916803906	26.016417804459397	27.13824105229401	23.84566522644269
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	36813.0
1	24816.5
2	12820.0
3	11462.5
4	10105.0
5	9889.0
6	9673.0
7	9872.5
8	10462.5
9	10853.0
10	11774.5
11	12696.0
12	14458.5
13	16221.0
14	18895.5
15	21570.0
16	27113.0
17	32656.0
18	43166.5
19	53677.0
20	70327.0
21	86977.0
22	167803.0
23	248629.0
24	238290.0
25	265051.0
26	302151.0
27	351180.0
28	400209.0
29	459939.0
30	519669.0
31	591587.0
32	663505.0
33	757475.0
34	851445.0
35	944571.5
36	1037698.0
37	1151568.5
38	1265439.0
39	1398807.0
40	1532175.0
41	1658915.0
42	1910385.5
43	2035116.0
44	2131273.0
45	2227430.0
46	2292957.5
47	2358485.0
48	2424676.5
49	2490868.0
50	2499511.5
51	2508155.0
52	2452076.0
53	2395997.0
54	2268479.0
55	2140961.0
56	1942918.5
57	1744876.0
58	1557243.0
59	1188247.0
60	1006884.0
61	851024.0
62	695164.0
63	573075.5
64	450987.0
65	366024.5
66	281062.0
67	224546.5
68	168031.0
69	134740.0
70	101449.0
71	80046.0
72	58643.0
73	45010.0
74	31377.0
75	24171.0
76	12713.0
77	8461.0
78	6385.0
79	4309.0
80	3468.5
81	2628.0
82	1922.0
83	1216.0
84	829.5
85	443.0
86	308.5
87	174.0
88	136.0
89	98.0
90	76.5
91	55.0
92	39.5
93	18.5
94	13.0
95	8.5
96	4.0
97	4.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	3.1248149E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.38460038739282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.0230296609078	56.673275896051734
2	6.3179957797528745	8.135632670572464
3	2.040843799935701	3.941967375358452
4	0.9770641228306463	2.5163153240523863
5	0.5835179267332401	1.8784784265799819
6	0.38431971209618765	1.4846562650586541
7	0.2601226693226597	1.172352588122896
8	0.2071388806361199	1.0669243243558753
9	0.15085054817264343	0.8741207036083319
>10	0.9122777587777684	11.062476981368558
>50	0.08920152129237363	3.984948483776005
>100	0.0519305332879284	6.000217986183002
>500	0.0015711472390799765	0.6394429778409949
>1k	1.2097755237139194E-4	0.1479474620855978
>5k	0.0	0.0
>10k+	1.4961462424754722E-5	0.42124253498496084
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100111	0.32037417640321675	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.1203032858042246E-5	0.0	0.0	0.0	0.0
2	5.4403222411669886E-5	3.2001895536276404E-6	0.0	0.0	0.0
3	5.4403222411669886E-5	9.600568660882922E-6	0.0	0.0	0.0
4	5.7603411965297525E-5	9.600568660882922E-6	0.0	0.0	0.0
5	5.7603411965297525E-5	1.60009477681382E-5	0.0	0.0	0.0
6	5.7603411965297525E-5	1.60009477681382E-5	0.0	0.0	0.0
7	5.7603411965297525E-5	1.60009477681382E-5	0.0	0.0	0.0
8	5.7603411965297525E-5	1.60009477681382E-5	0.0	0.0	0.0
9	6.080360151892517E-5	1.9201137321765844E-5	0.0	0.0	0.0
10	6.080360151892517E-5	2.2401326875393484E-5	0.0	0.0	0.0
11	6.40037910725528E-5	2.2401326875393484E-5	0.0	0.0	0.0
12	7.360435973343573E-5	2.2401326875393484E-5	0.0	0.0	0.0
13	7.360435973343573E-5	2.2401326875393484E-5	0.0	0.0	0.0
14	9.600568660882921E-5	2.2401326875393484E-5	0.0	0.0	0.0
15	1.0240606571608449E-4	2.5601516429021123E-5	0.0	0.0	0.0
16	1.184070134842227E-4	2.5601516429021123E-5	0.0	0.0	0.0
17	1.280075821451056E-4	2.5601516429021123E-5	0.0	6.400379107255281E-6	0.0
18	1.4720871946687146E-4	2.5601516429021123E-5	3.2001895536276404E-6	6.400379107255281E-6	0.0
19	1.6961004634226496E-4	2.5601516429021123E-5	3.2001895536276404E-6	1.2800758214510562E-5	6.400379107255281E-6
20	1.8241080455677551E-4	2.5601516429021123E-5	3.2001895536276404E-6	1.60009477681382E-5	6.400379107255281E-6
21	1.9841175232491372E-4	2.5601516429021123E-5	3.2001895536276404E-6	2.2401326875393484E-5	6.400379107255281E-6
22	2.304136478611901E-4	2.5601516429021123E-5	3.2001895536276404E-6	2.8801705982648763E-5	6.400379107255281E-6
23	2.49614785182956E-4	2.5601516429021123E-5	3.2001895536276404E-6	2.8801705982648763E-5	6.400379107255281E-6
24	2.944174389337429E-4	2.5601516429021123E-5	3.2001895536276404E-6	3.20018955362764E-5	9.600568660882922E-6
25	3.168187658091364E-4	2.5601516429021123E-5	3.2001895536276404E-6	3.840227464353169E-5	9.600568660882922E-6
26	3.552210404526681E-4	2.5601516429021123E-5	3.2001895536276404E-6	4.160246419715933E-5	1.2800758214510562E-5
27	4.000236942034551E-4	2.5601516429021123E-5	3.2001895536276404E-6	4.160246419715933E-5	1.9201137321765844E-5
28	4.3842596884698673E-4	3.20018955362764E-5	3.2001895536276404E-6	4.480265375078697E-5	1.9201137321765844E-5
29	5.088301390267948E-4	3.20018955362764E-5	3.2001895536276404E-6	5.4403222411669886E-5	1.9201137321765844E-5
30	5.472324136703265E-4	3.520208508990405E-5	3.2001895536276404E-6	5.4403222411669886E-5	1.9201137321765844E-5
31	6.080360151892517E-4	3.840227464353169E-5	6.400379107255281E-6	6.720398062618045E-5	1.9201137321765844E-5
32	7.072418913517085E-4	3.840227464353169E-5	6.400379107255281E-6	8.000473884069101E-5	1.9201137321765844E-5
33	8.512504212649524E-4	4.160246419715933E-5	6.400379107255281E-6	9.280549705520157E-5	1.9201137321765844E-5
34	9.632570556419198E-4	4.160246419715933E-5	6.400379107255281E-6	1.0240606571608449E-4	1.9201137321765844E-5
35	0.0010464619840362384	4.160246419715933E-5	6.400379107255281E-6	1.1520682393059505E-4	1.9201137321765844E-5
36	0.001123266533323302	4.160246419715933E-5	6.400379107255281E-6	1.440085299132438E-4	1.9201137321765844E-5
37	0.001238473357253897	4.160246419715933E-5	6.400379107255281E-6	1.6320966723500966E-4	1.9201137321765844E-5
38	0.0013504799916308644	4.160246419715933E-5	6.400379107255281E-6	1.8881118366403078E-4	2.2401326875393484E-5
39	0.0015008889006513633	4.480265375078697E-5	6.400379107255281E-6	1.9201137321765843E-4	2.2401326875393484E-5
40	0.0016864998947617666	4.480265375078697E-5	6.400379107255281E-6	2.0801232098579663E-4	2.2401326875393484E-5
41	0.0018625103202112867	4.800284330441461E-5	6.400379107255281E-6	2.304136478611901E-4	2.2401326875393484E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	56995	0.0	41.23581	12
CAACGCA	57980	0.0	40.515007	13
ATCAACG	58250	0.0	40.363518	11
AACGCAG	58680	0.0	39.99966	14
GGTATCA	59250	0.0	39.789368	8
GTATCAA	59290	0.0	39.71892	9
AGTGGTA	60045	0.0	39.05904	5
GTGGTAT	61485	0.0	38.239815	6
ACGCAGA	61445	0.0	38.203518	15
TATCAAC	61995	0.0	38.016212	10
AAGCAGT	61995	0.0	37.982098	1
AGAGTAC	60315	0.0	37.86728	19
TGGTATC	62515	0.0	37.651123	7
CGCAGAG	63740	0.0	36.86484	16
GCAGTGG	67165	0.0	35.09343	3
CAGTGGT	68735	0.0	34.267914	4
CAGAGTA	67835	0.0	34.02624	18
AGCAGTG	69610	0.0	33.813534	2
ACATGGG	38695	0.0	33.20791	24
GCAGAGT	70660	0.0	33.164734	17
>>END_MODULE