FastQCFastQC Report
Thu 26 May 2016
SRR805268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7710714
Sequences flagged as poor quality0
Sequence length53
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT258870.33572766413071475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC273000.041.64578612
ATCAACG271350.041.51796711
CAACGCA275650.041.2454213
GTATCAA268400.041.168789
GGTATCA263950.040.8924068
AGTGGTA261500.040.8711285
AACGCAG266750.040.80674714
AAGCAGT254100.040.535421
TGGTATC272700.040.1490637
TATCAAC292700.040.12743810
GTGGTAT268350.040.002986
GCAGTGG253950.039.994883
CAGTGGT275300.039.223574
AGAGTAC278750.038.38403319
ACGCAGA288800.038.31769615
ACATGGG90250.037.96454224
GTACTTT137250.037.73697722
GAGTACT153400.037.1036520
CGCAGAG295050.036.92458716
AGCAGTG270350.036.8906972
Produced by FastQC (version 0.11.5)