##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805268_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7710714
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44715016015378	35.0	33.0	36.0	17.0	37.0
2	32.07437287908746	35.0	32.0	35.0	23.0	37.0
3	33.69674196189873	37.0	32.0	39.0	25.0	39.0
4	34.64272724938313	37.0	34.0	39.0	28.0	39.0
5	34.652718671707966	37.0	34.0	39.0	28.0	39.0
6	34.40205420665323	37.0	34.0	38.0	27.0	39.0
7	34.689020497972045	37.0	34.0	38.0	28.0	39.0
8	34.98237452459007	37.0	33.0	39.0	27.0	40.0
9	34.649468648428666	37.0	33.0	39.0	27.0	40.0
10	35.059732470948866	37.0	33.0	38.0	27.0	40.0
11	34.36869322348099	36.0	33.0	38.0	27.0	40.0
12	33.90260460963797	36.0	32.0	38.0	27.0	39.0
13	33.78131415586157	36.0	32.0	38.0	26.0	39.0
14	33.10783424206889	35.0	31.0	38.0	25.0	39.0
15	32.6631031834406	34.0	31.0	37.0	24.0	39.0
16	32.665278468375305	34.0	31.0	37.0	25.0	38.0
17	31.15356956567187	33.0	29.0	36.0	19.0	38.0
18	31.23888021264957	33.0	29.0	36.0	21.0	38.0
19	30.50798486365854	32.0	27.0	36.0	19.0	37.0
20	28.986420323720996	32.0	27.0	34.0	16.0	37.0
21	28.693163564359928	31.0	26.0	34.0	16.0	37.0
22	27.71576082318706	30.0	24.0	34.0	11.0	36.0
23	27.223701722045455	30.0	24.0	33.0	11.0	36.0
24	26.28887674993522	29.0	23.0	32.0	10.0	35.0
25	26.047851470045448	27.0	23.0	32.0	10.0	34.0
26	25.307437936357125	27.0	19.0	31.0	10.0	34.0
27	24.485170634003545	27.0	18.0	30.0	10.0	34.0
28	23.707732902556106	25.0	16.0	30.0	10.0	34.0
29	22.95416299969108	25.0	16.0	30.0	10.0	33.0
30	22.338335593824386	24.0	16.0	30.0	10.0	33.0
31	21.74780026856138	24.0	15.0	30.0	10.0	31.0
32	21.04775848254779	23.0	15.0	27.0	10.0	31.0
33	20.24086277872581	19.0	10.0	27.0	10.0	30.0
34	19.669811381929094	19.0	10.0	26.0	10.0	30.0
35	18.949991790643512	18.0	10.0	25.0	9.0	30.0
36	18.286113062940736	16.0	10.0	25.0	9.0	30.0
37	17.819986190643306	16.0	10.0	24.0	9.0	30.0
38	17.092478854746783	15.0	10.0	24.0	9.0	28.0
39	16.507210745982796	15.0	10.0	24.0	9.0	27.0
40	16.024014896674938	15.0	10.0	23.0	9.0	26.0
41	15.51548818955028	14.0	10.0	22.0	9.0	25.0
42	15.136360394121738	13.0	10.0	21.0	9.0	25.0
43	14.701878580894064	13.0	10.0	19.0	9.0	24.0
44	14.37690387686536	12.0	10.0	18.0	9.0	24.0
45	14.014041630904739	10.0	9.0	16.0	8.0	24.0
46	13.722990374172872	10.0	9.0	15.0	8.0	24.0
47	13.38663021349255	10.0	9.0	15.0	8.0	23.0
48	13.137934178339385	10.0	9.0	15.0	8.0	23.0
49	12.904697671318116	10.0	9.0	15.0	8.0	23.0
50	12.733297590858642	10.0	9.0	15.0	8.0	23.0
51	12.534987032329301	10.0	9.0	14.0	8.0	22.0
52	12.338002031977842	10.0	9.0	14.0	8.0	22.0
53	12.041140937142787	10.0	9.0	14.0	8.0	21.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
10	4.0
11	8.0
12	175.0
13	956.0
14	3916.0
15	12094.0
16	30486.0
17	66891.0
18	133397.0
19	237596.0
20	385450.0
21	573658.0
22	795902.0
23	1038611.0
24	1270429.0
25	1347986.0
26	1020835.0
27	469992.0
28	157146.0
29	65057.0
30	38086.0
31	26277.0
32	17770.0
33	11049.0
34	5101.0
35	1560.0
36	248.0
37	33.0
38	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.73990061101994	41.48805156046509	16.821542077685674	23.9505057508293
2	25.750352042625362	30.882198457886002	27.790487366020837	15.576962133467795
3	22.174690955986694	24.339704468354036	30.427752864391028	23.057851711268242
4	18.775433766574665	28.77363886145952	27.36601305663782	25.084914315327993
5	20.052864105710576	31.43108407340747	27.113325692017625	21.40272612886433
6	23.162096272796525	25.426568797649608	29.864393881033585	21.546941048520278
7	21.087320836954916	26.434166797004792	30.577531471145214	21.900980894895078
8	22.049631201468504	25.34364521884744	30.273448087946203	22.33327549173786
9	23.31612870092186	25.030289023818025	30.007052524578135	21.64652975068197
10	21.046001706197377	26.516208486010505	31.344697780257448	21.093092027534674
11	20.982583454658023	29.643765804308135	27.940317848645403	21.433332892388435
12	20.871102209211752	25.83195278673285	31.410489352866673	21.886455651188722
13	20.978718702314726	26.615900939912958	27.61455035162762	24.790830006144695
14	21.205960952513607	29.301281308060446	27.309675861405314	22.183081878020637
15	20.958487112866592	29.345310953045335	27.465251596674445	22.23095033741363
16	21.683724749744318	26.20119226312894	26.985490578434113	25.12959240869263
17	24.33832975778897	26.099191332994582	27.81678843230341	21.745690476913033
18	21.309336593213025	26.00582773527847	27.060529024938546	25.624306646569956
19	21.73375902672567	29.50230030578232	26.740532718500514	22.023407948991494
20	24.618394613002117	26.115713797710562	27.57612330064375	21.689768288643567
21	21.68233707021166	29.334339206459997	26.72066426014504	22.262659463183308
22	24.39565259455869	26.075082022235552	27.509838907265916	22.01942647593984
23	21.40476225677674	26.29353131240505	30.759434210632115	21.542272220186096
24	21.529796073359744	29.30825861262654	26.811447033309754	22.350498280703963
25	21.355765497202984	26.41389630065387	26.726578109368344	25.503760092774808
26	21.498761333905005	27.550483651708518	29.003889912140433	21.946865102246043
27	21.659472780341744	25.7638916447945	30.286961752180147	22.289673822683607
28	23.03074656899478	25.990057470683	28.775337796214462	22.20385816410776
29	22.488643723525474	25.9859463079554	29.498707901758515	22.026702066760613
30	23.017712756561846	25.788377055613786	28.892564294305302	22.30134589351907
31	22.20820276825207	26.60706907298079	28.89460042221771	22.29012773654943
32	21.872060097158318	26.55488194737867	29.239380425729706	22.333677529733304
33	21.6901962645742	26.267969477275386	29.346309563550143	22.695524694600266
34	21.886325961512775	26.688643879153084	28.85077828071434	22.5742518786198
35	21.536539936509122	26.43092455510605	29.513803780039048	22.518731728345777
36	21.443798849237567	26.239178369214578	29.514581918094745	22.802440863453114
37	21.561544106032205	26.5409143692789	29.30462730169995	22.592914222988945
38	21.281764568106144	26.309158918356978	29.752679194170607	22.656397319366274
39	21.18335604199559	26.82668557023383	29.41551197463685	22.574446413133725
40	21.370848406515922	26.50093104218364	29.344934852985084	22.783285698315357
41	21.27895030213804	26.394300190617887	29.647954780841307	22.678794726402767
42	21.28588869980134	26.23312186134773	29.61553236185391	22.86545707699702
43	21.27323098742866	26.43705887677847	29.22593160633373	23.06377852945914
44	21.398135114335716	26.42227425372021	29.501599981532188	22.677990650411882
45	20.9916617319745	26.3085753148152	29.621874187007847	23.077888766202456
46	21.581918354123886	26.357079253620352	28.985305381576854	23.07569701067891
47	21.42040283169626	26.272067671035394	29.315728737961233	22.991800759307115
48	21.32183867797457	26.403404405869544	29.119573103087472	23.155183813068415
49	21.74943850854798	26.42584071980883	28.84077920669863	22.98394156494457
50	21.447326408423393	26.543209876543212	28.843243310541673	23.166220404491725
51	21.5376293297871	26.37208434912772	28.883045072090603	23.20724124899458
52	21.80389520347921	26.63141182515653	28.45088016492377	23.113812806440492
53	21.703074449395995	26.673081118039132	28.37584171841933	23.24800271414554
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13383.0
1	12191.5
2	11000.0
3	10429.5
4	9859.0
5	9518.5
6	9178.0
7	9020.5
8	8897.0
9	8931.0
10	9150.0
11	9369.0
12	9867.0
13	10365.0
14	11214.0
15	12063.0
16	13821.0
17	15579.0
18	19508.0
19	23437.0
20	30967.5
21	38498.0
22	63591.0
23	88684.0
24	94116.0
25	110581.0
26	121614.0
27	137812.5
28	154011.0
29	173263.0
30	192515.0
31	214073.0
32	235631.0
33	261221.5
34	286812.0
35	312475.5
36	338139.0
37	364794.5
38	391450.0
39	419585.5
40	447721.0
41	475850.0
42	525687.0
43	547395.0
44	564102.0
45	580809.0
46	586812.0
47	592815.0
48	587307.0
49	581799.0
50	565481.5
51	549164.0
52	517945.5
53	486727.0
54	445888.0
55	405049.0
56	357383.0
57	309717.0
58	266130.0
59	186970.0
60	151397.0
61	125295.0
62	99193.0
63	80834.5
64	62476.0
65	50188.0
66	37900.0
67	30260.0
68	22620.0
69	18003.5
70	13387.0
71	10436.5
72	7486.0
73	5971.5
74	4457.0
75	3437.5
76	1829.5
77	1241.0
78	980.5
79	720.0
80	586.5
81	453.0
82	328.5
83	204.0
84	140.5
85	77.0
86	50.5
87	24.0
88	15.0
89	6.0
90	5.0
91	4.0
92	2.5
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	7710714.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.41856378882298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.93683644526075	74.85365182661047
2	4.635584811153878	7.5484531529086185
3	1.3856819091895847	3.384606927431107
4	0.6354220798759265	2.0694061257281877
5	0.36994779684466816	1.5060309147966071
6	0.23229454809003477	1.1347853088878583
7	0.1633583467739235	0.931028138407455
8	0.12022292583209063	0.7830702364591204
9	0.08696244003156355	0.6372321273847396
>10	0.4209019331797797	5.726425462437151
>50	0.010637259212427669	0.5783925427308984
>100	0.001972066031244367	0.26691434961861044
>500	8.066581828742885E-5	0.04874179971242191
>1k	4.8386353703212234E-5	0.05200202593250322
>5k	3.2257568113633515E-5	0.1393155243770261
>10k+	1.6128784056816758E-5	0.3399435365772528
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25887	0.33572766413071475	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5937935189918858E-5	0.0	0.0	0.0	0.0
2	2.5937935189918858E-5	0.0	0.0	0.0	0.0
3	2.5937935189918858E-5	0.0	0.0	3.890690278487829E-5	0.0
4	2.5937935189918858E-5	0.0	0.0	3.890690278487829E-5	0.0
5	2.5937935189918858E-5	0.0	0.0	3.890690278487829E-5	0.0
6	2.5937935189918858E-5	0.0	0.0	5.1875870379837716E-5	0.0
7	2.5937935189918858E-5	0.0	0.0	6.484483797479714E-5	0.0
8	2.5937935189918858E-5	0.0	1.2968967594959429E-5	7.781380556975658E-5	0.0
9	2.5937935189918858E-5	0.0	1.2968967594959429E-5	9.0782773164716E-5	0.0
10	2.5937935189918858E-5	0.0	1.2968967594959429E-5	1.0375174075967543E-4	0.0
11	2.5937935189918858E-5	0.0	1.2968967594959429E-5	1.1672070835463486E-4	0.0
12	5.1875870379837716E-5	0.0	1.2968967594959429E-5	1.1672070835463486E-4	0.0
13	1.9453451392439144E-4	0.0	1.2968967594959429E-5	1.1672070835463486E-4	0.0
14	1.9453451392439144E-4	0.0	1.2968967594959429E-5	1.2968967594959428E-4	0.0
15	2.853172870891074E-4	0.0	1.2968967594959429E-5	1.426586435445537E-4	0.0
16	2.982862546840669E-4	0.0	1.2968967594959429E-5	1.426586435445537E-4	0.0
17	3.112552222790263E-4	0.0	1.2968967594959429E-5	1.426586435445537E-4	0.0
18	3.63131092658864E-4	0.0	1.2968967594959429E-5	1.5562761113951316E-4	0.0
19	3.7610006025382345E-4	0.0	5.1875870379837716E-5	1.5562761113951316E-4	0.0
20	3.890690278487829E-4	0.0	5.1875870379837716E-5	1.5562761113951316E-4	1.2968967594959429E-5
21	4.2797593063366115E-4	0.0	5.1875870379837716E-5	1.685965787344726E-4	1.2968967594959429E-5
22	5.057897362034178E-4	0.0	5.1875870379837716E-5	1.685965787344726E-4	1.2968967594959429E-5
23	5.965725093681338E-4	0.0	5.1875870379837716E-5	1.9453451392439144E-4	1.2968967594959429E-5
24	6.095414769630932E-4	0.0	5.1875870379837716E-5	2.204724491143103E-4	1.2968967594959429E-5
25	6.873552825328497E-4	0.0	5.1875870379837716E-5	2.204724491143103E-4	1.2968967594959429E-5
26	7.392311529126874E-4	1.2968967594959429E-5	5.1875870379837716E-5	2.204724491143103E-4	1.2968967594959429E-5
27	8.429828936723629E-4	1.2968967594959429E-5	5.1875870379837716E-5	2.4641038430422914E-4	1.2968967594959429E-5
28	8.818897964572412E-4	1.2968967594959429E-5	5.1875870379837716E-5	2.5937935189918857E-4	1.2968967594959429E-5
29	9.856415372169166E-4	1.2968967594959429E-5	5.1875870379837716E-5	2.5937935189918857E-4	1.2968967594959429E-5
30	0.0010504863751917138	1.2968967594959429E-5	5.1875870379837716E-5	3.2422418987398575E-4	1.2968967594959429E-5
31	0.0011412691483564297	1.2968967594959429E-5	5.1875870379837716E-5	3.7610006025382345E-4	1.2968967594959429E-5
32	0.0013487726298757807	1.2968967594959429E-5	5.1875870379837716E-5	4.150069630387017E-4	1.2968967594959429E-5
33	0.0017119037225346446	1.2968967594959429E-5	5.1875870379837716E-5	4.7985180101349885E-4	1.2968967594959429E-5
34	0.0021917555235481437	1.2968967594959429E-5	5.1875870379837716E-5	5.317276713933366E-4	1.2968967594959429E-5
35	0.00272348319494148	1.2968967594959429E-5	5.1875870379837716E-5	5.836035417731743E-4	1.2968967594959429E-5
36	0.003151459125575141	1.2968967594959429E-5	5.1875870379837716E-5	7.132932177227686E-4	1.2968967594959429E-5
37	0.003333024671904573	1.2968967594959429E-5	5.1875870379837716E-5	7.911070232925252E-4	1.2968967594959429E-5
38	0.003333024671904573	1.2968967594959429E-5	5.1875870379837716E-5	9.207966992421195E-4	1.2968967594959429E-5
39	0.0033589626070944924	1.2968967594959429E-5	5.1875870379837716E-5	9.597036020269977E-4	1.2968967594959429E-5
40	0.00341083847747433	1.2968967594959429E-5	5.1875870379837716E-5	0.0010634553427866732	1.2968967594959429E-5
41	0.003423807445069289	1.2968967594959429E-5	5.1875870379837716E-5	0.0011542381159513893	1.2968967594959429E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	27300	0.0	41.645786	12
ATCAACG	27135	0.0	41.517967	11
CAACGCA	27565	0.0	41.24542	13
GTATCAA	26840	0.0	41.16878	9
GGTATCA	26395	0.0	40.892406	8
AGTGGTA	26150	0.0	40.871128	5
AACGCAG	26675	0.0	40.806747	14
AAGCAGT	25410	0.0	40.53542	1
TGGTATC	27270	0.0	40.149063	7
TATCAAC	29270	0.0	40.127438	10
GTGGTAT	26835	0.0	40.00298	6
GCAGTGG	25395	0.0	39.99488	3
CAGTGGT	27530	0.0	39.22357	4
AGAGTAC	27875	0.0	38.384033	19
ACGCAGA	28880	0.0	38.317696	15
ACATGGG	9025	0.0	37.964542	24
GTACTTT	13725	0.0	37.736977	22
GAGTACT	15340	0.0	37.10365	20
CGCAGAG	29505	0.0	36.924587	16
AGCAGTG	27035	0.0	36.890697	2
>>END_MODULE