FastQCFastQC Report
Thu 26 May 2016
SRR805267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6118081
Sequences flagged as poor quality0
Sequence length53
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT216940.35458830963499827No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69520.11363040142816022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG117850.040.7585924
GTACTTT139600.039.929822
AGTACTT153150.038.99020821
GAGTACT168350.038.68042820
CATGGGG56650.038.6619625
TACTTTT143550.038.2908423
ACTTTTT149550.036.45603624
AGAGTAC409750.034.32519519
GTATCAA443100.033.0994159
TATCAAC490950.031.82635910
CATGGGA39750.031.80628825
TACATGG167400.031.76851723
CAGTGGT459000.031.7070834
GCAGTGG439100.031.6562293
AGTGGTA455650.031.6101175
TCAACGC485450.031.518912
ATCAACG480000.031.50468611
GTGGTAT460700.031.4370546
TGGTATC469750.031.356687
CAACGCA492150.031.17575813
Produced by FastQC (version 0.11.5)