##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805267_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6118081
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.913338185617352	35.0	29.0	35.0	17.0	37.0
2	30.797097161675367	35.0	28.0	35.0	17.0	37.0
3	32.33087106888581	35.0	30.0	37.0	17.0	39.0
4	33.21587994666955	35.0	32.0	37.0	25.0	39.0
5	33.41276374078735	35.0	32.0	37.0	25.0	39.0
6	33.18089789919421	35.0	32.0	37.0	25.0	39.0
7	33.35470043629694	35.0	32.0	37.0	25.0	39.0
8	33.683865741561775	36.0	32.0	38.0	25.0	40.0
9	33.29194971429767	36.0	32.0	38.0	24.0	40.0
10	33.56616151371647	36.0	32.0	38.0	25.0	40.0
11	33.13529438397432	36.0	32.0	38.0	25.0	39.0
12	32.66864086957985	35.0	31.0	38.0	24.0	39.0
13	32.54656615366812	35.0	31.0	38.0	23.0	39.0
14	32.04546605381655	34.0	30.0	37.0	19.0	39.0
15	31.609428021629657	34.0	29.0	37.0	18.0	39.0
16	31.427593390803423	34.0	29.0	36.0	19.0	38.0
17	30.019279411305604	32.0	27.0	36.0	18.0	38.0
18	30.05407741414342	32.0	27.0	36.0	18.0	37.0
19	29.418848001522047	32.0	27.0	35.0	17.0	37.0
20	27.85277622182511	31.0	24.0	34.0	10.0	37.0
21	27.41249682702795	30.0	24.0	34.0	10.0	36.0
22	26.649477017385028	29.0	21.0	33.0	10.0	36.0
23	25.889728168031773	27.0	19.0	32.0	10.0	35.0
24	25.25475422767368	27.0	18.0	32.0	10.0	34.0
25	24.78136739281484	27.0	18.0	31.0	10.0	34.0
26	24.391348855956632	27.0	17.0	31.0	10.0	34.0
27	23.381667388843006	25.0	16.0	30.0	10.0	34.0
28	22.772677413064653	25.0	16.0	30.0	10.0	33.0
29	21.903848118388755	24.0	15.0	30.0	10.0	33.0
30	21.493087129771574	24.0	15.0	30.0	10.0	32.0
31	20.94495283733576	23.0	14.0	27.0	10.0	31.0
32	20.3970673157155	21.0	10.0	27.0	10.0	30.0
33	19.611548457759877	19.0	10.0	26.0	9.0	30.0
34	19.02293954591317	18.0	10.0	25.0	9.0	30.0
35	18.346713618208064	16.0	10.0	25.0	9.0	30.0
36	17.773740981853624	16.0	10.0	24.0	9.0	30.0
37	17.293482711327293	15.0	10.0	24.0	9.0	30.0
38	16.66444559985394	15.0	10.0	24.0	9.0	27.0
39	16.099953563870763	15.0	10.0	23.0	9.0	27.0
40	15.70096898030608	15.0	10.0	23.0	9.0	25.0
41	15.22841116356583	13.0	10.0	21.0	9.0	25.0
42	14.795637553670833	13.0	10.0	19.0	9.0	24.0
43	14.400298230768765	12.0	10.0	19.0	8.0	24.0
44	14.081765834744587	11.0	9.0	17.0	8.0	24.0
45	13.72659858540611	10.0	9.0	15.0	8.0	24.0
46	13.456997055122349	10.0	9.0	15.0	8.0	23.0
47	13.128399574964764	10.0	9.0	15.0	8.0	23.0
48	12.866948639614284	10.0	9.0	15.0	8.0	23.0
49	12.627963081887932	10.0	9.0	15.0	8.0	23.0
50	12.472057169560193	10.0	9.0	14.0	8.0	22.0
51	12.264952196611977	10.0	9.0	14.0	8.0	22.0
52	12.080366376319633	10.0	9.0	14.0	8.0	22.0
53	11.779591836067551	10.0	9.0	13.0	8.0	20.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
8	1.0
9	25.0
10	181.0
11	869.0
12	3111.0
13	7677.0
14	16650.0
15	33736.0
16	65330.0
17	117045.0
18	199510.0
19	313219.0
20	455022.0
21	602804.0
22	742989.0
23	851077.0
24	897765.0
25	820997.0
26	554752.0
27	247628.0
28	87416.0
29	38802.0
30	23670.0
31	16184.0
32	11198.0
33	6578.0
34	2971.0
35	778.0
36	83.0
37	13.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.810218923221186	37.49111853863981	22.871975706107847	23.826686832031154
2	25.74707657515486	35.81971209599873	20.70353432718527	17.72967700166114
3	27.52410437194277	26.107091422947814	24.54307159385435	21.825732611255066
4	20.73092853788631	26.331344746825025	24.010208429734746	28.927518285553916
5	21.535085919915083	32.47456187650997	24.543136973832155	21.447215229742792
6	26.644972500364084	25.13649296241746	25.59279617252534	22.625738364693113
7	22.05959679186987	25.312888142540118	30.805901392936775	21.82161367265324
8	26.119775138642332	25.31386884220722	26.75353595351222	21.812820065638228
9	26.407953735820104	24.926574198674388	26.869242169235747	21.79622989626976
10	21.43543048874312	25.874142562022307	31.10967311482146	21.58075383441311
11	21.1168502018852	32.207680807102754	25.092148992470026	21.583319998542027
12	20.882413946464588	24.971228723516408	31.75159008192275	22.394767248096258
13	21.146009671987017	25.81597072676874	25.751211858751134	27.286807742493114
14	21.371701355375976	31.709076751353898	24.758792830627772	22.160429062642354
15	20.71067708976066	31.36553765796824	25.3914258408805	22.532359411390598
16	21.85343737685068	25.39147487586385	25.266844293169704	27.488243454115757
17	26.714357001811518	25.542273794675157	25.917129897430257	21.826239306083068
18	21.197806959404428	25.34817698556132	25.311858407889666	28.14215764714459
19	21.80597151296297	31.153755564857672	25.147558523661257	21.8927143985181
20	26.89925157904905	25.6663323025635	25.73959056769598	21.694825550691466
21	21.534007150281273	31.17016593928717	24.900340482579423	22.39548642785213
22	27.019534393219054	25.05110671140183	26.089896488784635	21.839462406594485
23	21.210376260137778	25.718979529692398	31.34494296495911	21.72570124521071
24	21.344683079547327	31.280151406952605	24.878748744908737	22.496416768591327
25	21.35097590241123	25.16808783669258	25.42807131844119	28.052864942454995
26	21.100488862439057	28.519122254183948	28.211591183575376	22.16879769980162
27	21.436460223393578	25.266468358297317	31.169740969431427	22.12733044887768
28	24.648186253173176	25.22990460570888	27.992274048022576	22.129635093095366
29	23.33385255932375	25.2711757166994	29.242077703776726	22.152894020200126
30	24.33223751042198	25.255353762070165	28.216396611944173	22.196012115563686
31	22.41856556001792	26.05879196434307	28.60898703367935	22.91365544195966
32	22.163420196627015	26.405158741768865	28.894566776739307	22.536854284864813
33	22.06832501890707	25.81103453844433	29.038500797880907	23.082139644767697
34	21.950755473816056	26.317696676457864	28.818905797422428	22.912642052303656
35	21.968244617879364	25.889997206640448	29.053178602898523	23.088579572581665
36	21.473988330654663	26.018043893175	29.45114652780831	23.056821248362027
37	21.841080561045203	26.012159695172393	29.142945312427216	23.00381443135519
38	21.581080734302144	25.968812769886508	29.36477957712557	23.085326918685777
39	21.54314400217977	26.393946075575002	29.075881800191922	22.987028122053303
40	21.427519511428503	26.00249980345144	29.192307195671326	23.377673489448732
41	21.591982845601425	26.210865792721606	29.181274324416428	23.01587703726054
42	21.610158479431703	25.732954499948598	29.34245231470456	23.314434705915136
43	21.518544785529972	26.150291243283636	29.114276192158943	23.21688777902744
44	21.58577174770978	26.009119526204376	29.11180809799674	23.293300628089103
45	21.357203345297325	25.916198232746513	29.40281437921466	23.323784042741508
46	21.67839883126752	25.82796795269628	28.968544221627663	23.52508899440854
47	21.488600755694474	26.241202102423944	28.67729276549297	23.59290437638861
48	21.783431765614086	25.87087356313197	28.850647776647616	23.495046894606332
49	21.604061796501224	25.92180456584344	28.912415510680557	23.56171812697478
50	21.54768791063734	26.197985937093677	28.68291544358435	23.571410708684635
51	21.86984775128018	26.142592750896892	28.281776589750933	23.705782908071992
52	21.6888596277166	26.02499051581697	28.493591372850407	23.792558483616023
53	21.99547537863588	26.502950843573338	27.902605408460595	23.598968369330187
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10291.0
1	10431.5
2	10572.0
3	10135.5
4	9699.0
5	8904.0
6	8109.0
7	7413.0
8	6147.0
9	5577.0
10	5167.5
11	4758.0
12	4683.0
13	4608.0
14	4704.5
15	4801.0
16	5486.5
17	6172.0
18	8131.0
19	10090.0
20	14720.5
21	19351.0
22	39057.5
23	58764.0
24	61619.0
25	70975.0
26	77476.0
27	86637.5
28	95799.0
29	108049.0
30	120299.0
31	135493.5
32	150688.0
33	168286.0
34	185884.0
35	204879.0
36	223874.0
37	245471.5
38	267069.0
39	293118.5
40	319168.0
41	348146.5
42	408327.0
43	439529.0
44	469166.0
45	498803.0
46	514276.5
47	529750.0
48	525644.5
49	521539.0
50	503270.0
51	485001.0
52	456470.0
53	427939.0
54	391538.0
55	355137.0
56	313954.0
57	272771.0
58	235010.5
59	166001.0
60	134752.0
61	111181.0
62	87610.0
63	71122.5
64	54635.0
65	43375.5
66	32116.0
67	25175.0
68	18234.0
69	14185.5
70	10137.0
71	7794.0
72	5451.0
73	4212.5
74	2974.0
75	2259.5
76	1127.5
77	710.0
78	557.5
79	405.0
80	332.0
81	259.0
82	187.5
83	116.0
84	75.5
85	35.0
86	24.0
87	13.0
88	7.5
89	2.0
90	2.0
91	2.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	6118081.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.94722058469645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.83087529503649	81.58333811932683
2	3.7408098185044323	6.505060329098063
3	1.0747457682651465	2.8033847215745626
4	0.4784130971500722	1.6638675635406057
5	0.2625233741203818	1.1412838859141814
6	0.15861064606398817	0.8274452898244078
7	0.11276383956302681	0.686315170272467
8	0.07337848061732676	0.5104043952323667
9	0.05272037990819031	0.41255014510677795
>10	0.21069271354574232	2.802346489269446
>50	0.0027563135278037983	0.16354815370728867
>100	0.0015013557327889769	0.2714100191147811
>500	9.496327840727306E-5	0.06534411167669314
>1k	7.596979081345577E-5	0.09065859563341493
>5k	1.8992447689151576E-5	0.11480119424847539
>10k+	1.8992447689151576E-5	0.3582418164594973
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21694	0.35458830963499827	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6952	0.11363040142816022	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2689988903383265E-5	0.0	0.0	0.0	0.0
2	3.2689988903383265E-5	0.0	0.0	1.6344994451691633E-5	0.0
3	3.2689988903383265E-5	0.0	0.0	3.2689988903383265E-5	0.0
4	4.90349833550749E-5	0.0	0.0	3.2689988903383265E-5	0.0
5	4.90349833550749E-5	0.0	0.0	6.537997780676653E-5	0.0
6	4.90349833550749E-5	0.0	0.0	9.80699667101498E-5	0.0
7	4.90349833550749E-5	0.0	0.0	9.80699667101498E-5	0.0
8	4.90349833550749E-5	0.0	0.0	1.1441496116184143E-4	0.0
9	6.537997780676653E-5	0.0	0.0	1.3075995561353306E-4	0.0
10	6.537997780676653E-5	0.0	0.0	1.3075995561353306E-4	0.0
11	1.1441496116184143E-4	0.0	0.0	1.3075995561353306E-4	0.0
12	1.3075995561353306E-4	0.0	0.0	1.3075995561353306E-4	0.0
13	2.615199112270661E-4	0.0	0.0	1.3075995561353306E-4	0.0
14	2.615199112270661E-4	0.0	0.0	1.471049500652247E-4	0.0
15	4.086248612922908E-4	0.0	0.0	1.471049500652247E-4	0.0
16	4.249698557439825E-4	0.0	0.0	1.471049500652247E-4	0.0
17	4.5765984464736574E-4	0.0	1.6344994451691633E-5	1.471049500652247E-4	0.0
18	4.90349833550749E-4	0.0	1.6344994451691633E-5	1.471049500652247E-4	0.0
19	5.230398224541322E-4	0.0	1.6344994451691633E-5	1.7979493896860796E-4	0.0
20	5.720748058092071E-4	0.0	1.6344994451691633E-5	1.7979493896860796E-4	0.0
21	6.374547836159737E-4	0.0	1.6344994451691633E-5	1.7979493896860796E-4	0.0
22	6.864897669710486E-4	0.0	1.6344994451691633E-5	2.1248492787199124E-4	0.0
23	8.335947170362733E-4	0.0	1.6344994451691633E-5	2.2882992232368287E-4	0.0
24	8.989746948430399E-4	0.0	1.6344994451691633E-5	2.2882992232368287E-4	0.0
25	0.0010460796449082645	0.0	1.6344994451691633E-5	2.451749167753745E-4	0.0
26	0.0011441496116184142	0.0	1.6344994451691633E-5	2.451749167753745E-4	0.0
27	0.001291254561683639	0.0	1.6344994451691633E-5	2.451749167753745E-4	0.0
28	0.001471049500652247	0.0	1.6344994451691633E-5	2.615199112270661E-4	0.0
29	0.00160180945626578	0.0	1.6344994451691633E-5	2.7786490567875775E-4	0.0
30	0.0016835344285242382	0.0	1.6344994451691633E-5	2.942099001304494E-4	0.0
31	0.001781604395234388	0.0	1.6344994451691633E-5	3.432448834855243E-4	0.0
32	0.0018469843730411545	0.0	1.6344994451691633E-5	3.7593487238890756E-4	0.0
33	0.0019287093452996127	0.0	1.6344994451691633E-5	4.413148501956741E-4	0.0
34	0.0019940893231063794	0.0	1.6344994451691633E-5	5.230398224541322E-4	0.0
35	0.0021248492787199124	0.0	1.6344994451691633E-5	5.884198002608989E-4	0.0
36	0.002222919245430062	0.0	1.6344994451691633E-5	6.211097891642821E-4	0.0
37	0.002320989212140212	0.0	1.6344994451691633E-5	7.191797558744319E-4	0.0
38	0.0024844391566571284	0.0	1.6344994451691633E-5	7.682147392295067E-4	0.0
39	0.0025171291455605115	0.0	1.6344994451691633E-5	8.826297003913482E-4	0.0
40	0.002549819134463895	0.0	1.6344994451691633E-5	9.643546726498064E-4	0.0
41	0.0025988541178189697	0.0	1.6344994451691633E-5	9.80699667101498E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATGGG	11785	0.0	40.75859	24
GTACTTT	13960	0.0	39.9298	22
AGTACTT	15315	0.0	38.990208	21
GAGTACT	16835	0.0	38.680428	20
CATGGGG	5665	0.0	38.66196	25
TACTTTT	14355	0.0	38.29084	23
ACTTTTT	14955	0.0	36.456036	24
AGAGTAC	40975	0.0	34.325195	19
GTATCAA	44310	0.0	33.099415	9
TATCAAC	49095	0.0	31.826359	10
CATGGGA	3975	0.0	31.806288	25
TACATGG	16740	0.0	31.768517	23
CAGTGGT	45900	0.0	31.707083	4
GCAGTGG	43910	0.0	31.656229	3
AGTGGTA	45565	0.0	31.610117	5
TCAACGC	48545	0.0	31.5189	12
ATCAACG	48000	0.0	31.504686	11
GTGGTAT	46070	0.0	31.437054	6
TGGTATC	46975	0.0	31.35668	7
CAACGCA	49215	0.0	31.175758	13
>>END_MODULE