FastQCFastQC Report
Thu 26 May 2016
SRR805266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4185005
Sequences flagged as poor quality0
Sequence length53
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC25450.031.76424412
CAACGCA26300.031.09505713
ATCAACG25850.030.99999811
GGTATCA25950.029.250488
GTATCAA26900.029.0037179
AACGCAG27200.028.85661914
GATCGGA5400.028.7222231
AGTGGTA26700.027.4606725
TGGTATC30900.024.9449847
TATCAAC33300.024.84084310
GTGGTAT30250.024.3933876
AAGCAGT29000.024.1482751
GCAGTGG30600.022.962423
TATGCCG6750.022.2814816
ACGCAGA36800.021.77581615
CAGTGGT34400.021.587214
AGAGTAC36550.021.28180719
ACATGGG17700.020.97740224
CGCAGAG41600.019.43269316
GCCGTCT8350.018.5748519
Produced by FastQC (version 0.11.5)