##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805266_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4185005
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.301689484241955	35.0	33.0	37.0	17.0	37.0
2	31.875268010432485	35.0	32.0	35.0	19.0	37.0
3	33.58357301843128	35.0	32.0	38.0	25.0	39.0
4	34.561487501209676	37.0	34.0	39.0	28.0	39.0
5	34.49736666981282	37.0	34.0	38.0	28.0	39.0
6	34.237420504873946	37.0	33.0	38.0	27.0	39.0
7	34.62051706031414	37.0	34.0	38.0	28.0	39.0
8	34.856751903522216	37.0	33.0	39.0	27.0	40.0
9	34.560504228788254	37.0	33.0	38.0	27.0	40.0
10	35.10951958241388	36.0	33.0	38.0	29.0	40.0
11	34.297656753098266	36.0	33.0	38.0	27.0	40.0
12	33.81144753709972	36.0	32.0	38.0	27.0	39.0
13	33.668254876636944	36.0	32.0	38.0	26.0	39.0
14	32.951073893579576	35.0	31.0	37.0	25.0	39.0
15	32.52893819720646	34.0	31.0	37.0	24.0	39.0
16	32.56449753345576	34.0	31.0	37.0	25.0	38.0
17	31.19232832457787	33.0	29.0	36.0	19.0	38.0
18	31.140878445784413	33.0	29.0	36.0	21.0	38.0
19	30.455876157854053	32.0	27.0	36.0	18.0	37.0
20	28.98207313969756	32.0	27.0	34.0	16.0	37.0
21	28.6475086648642	31.0	26.0	34.0	16.0	37.0
22	27.58622677870158	30.0	24.0	34.0	11.0	36.0
23	27.267635044641523	30.0	24.0	33.0	15.0	36.0
24	26.146315237377255	29.0	23.0	32.0	10.0	35.0
25	26.000825805464988	27.0	23.0	32.0	10.0	34.0
26	25.141309747539133	27.0	19.0	31.0	10.0	34.0
27	24.428661136605573	26.0	18.0	30.0	10.0	34.0
28	23.588319488268233	25.0	16.0	30.0	10.0	33.0
29	22.86993587821281	25.0	16.0	30.0	10.0	33.0
30	22.152666245321093	24.0	16.0	30.0	10.0	32.0
31	21.51504526278941	24.0	15.0	29.0	10.0	31.0
32	20.775929777861677	23.0	15.0	27.0	10.0	30.0
33	20.00034312981705	19.0	10.0	26.0	10.0	30.0
34	19.466923217534983	19.0	10.0	25.0	9.0	30.0
35	18.72295254127534	18.0	10.0	25.0	9.0	30.0
36	18.05029862568862	16.0	10.0	24.0	9.0	30.0
37	17.61625685035024	16.0	10.0	24.0	9.0	30.0
38	16.86309096404903	15.0	10.0	24.0	9.0	27.0
39	16.255176517112883	15.0	10.0	23.0	9.0	26.0
40	15.813910377645906	15.0	10.0	23.0	9.0	25.0
41	15.295343494213268	14.0	10.0	22.0	9.0	25.0
42	14.956043063269936	13.0	10.0	20.0	9.0	24.0
43	14.51358218210014	12.0	10.0	19.0	9.0	24.0
44	14.192014585406708	12.0	9.0	18.0	8.0	24.0
45	13.816225309169283	10.0	9.0	15.0	8.0	24.0
46	13.552920247407112	10.0	9.0	15.0	8.0	23.0
47	13.218946213923282	10.0	9.0	15.0	8.0	23.0
48	12.978803131656951	10.0	9.0	15.0	8.0	23.0
49	12.739610824837724	10.0	9.0	15.0	8.0	23.0
50	12.57612213127583	10.0	9.0	14.0	8.0	22.0
51	12.387920444539493	10.0	9.0	14.0	8.0	22.0
52	12.190831074275897	10.0	9.0	14.0	8.0	22.0
53	11.848211889830479	10.0	9.0	13.0	8.0	20.0
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
10	1.0
11	8.0
12	79.0
13	453.0
14	1759.0
15	5581.0
16	14441.0
17	33882.0
18	70322.0
19	130354.0
20	217075.0
21	330584.0
22	462771.0
23	602462.0
24	718960.0
25	727187.0
26	515395.0
27	223274.0
28	72430.0
29	27678.0
30	13865.0
31	7863.0
32	4390.0
33	2449.0
34	1134.0
35	452.0
36	130.0
37	26.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.04754594080533	29.48677480672066	19.947120732233294	26.518558520240713
2	24.898679929892555	24.524200090561422	28.651076880433834	21.92604309911219
3	19.625639634839146	25.34587174925717	28.58352618455653	26.444962431347157
4	21.5414079553071	28.749404122575722	26.710242879040763	22.998945043076414
5	25.30290883762385	28.04491273009232	24.901523415145263	21.75065501713857
6	23.703412540725758	25.952776639454434	27.486179825352657	22.85763099446715
7	23.933902110033323	26.811867608282427	26.783504440257538	22.470725841426713
8	22.009292700964515	25.84754379027026	28.295545644509385	23.847617864255838
9	22.272685456767675	25.29846439848937	28.772175899431424	23.656674245311535
10	23.052039364349625	26.27918007266419	27.40515244306757	23.263628119918614
11	22.956770660966953	26.394018645139017	27.148641399472638	23.500569294421393
12	22.71586294401082	25.847615474772432	27.491962375194294	23.944559206022454
13	22.922720522436652	26.257818091017814	26.95905978606955	23.860401600475985
14	22.98632857069466	26.220255411881226	26.682262028360775	24.111153989063332
15	22.86824030078817	26.381808384936217	26.90457956442107	23.84537174985454
16	23.469697168820584	26.230554085359515	26.546706634759097	23.7530421110608
17	22.959303513376923	26.080207789476955	27.467780803129266	23.492707894016853
18	23.122290176475296	25.97913264141859	26.68144960400286	24.217127578103252
19	23.438801148385725	26.23676674221417	26.512799865233138	23.811632244166972
20	23.115097831424336	25.95241821694359	27.238605449694802	23.693878501937274
21	23.30654324188382	26.15349324552778	26.413612409065223	24.126351103523174
22	23.16936299956631	25.87970623691011	27.125415620769864	23.825515142753712
23	23.094285430961254	26.062286663934692	27.50457884757605	23.33884905752801
24	23.18730801994263	26.27263288813275	26.610051839842484	23.930007252082135
25	23.27741543916913	26.21053021442029	26.491724621595438	24.02032972481514
26	23.284368835879526	26.173397642296724	26.859967909237863	23.682265612585887
27	23.228048712008707	25.823242744034953	27.029310598195224	23.919397945761116
28	23.353639959808888	25.87299178854028	27.04603698203467	23.727331269616165
29	23.111250763141264	25.861020476678043	27.34245239850371	23.685276361676987
30	23.352158480097394	25.72240654431715	26.935475584855933	23.98995939072952
31	23.187188545772347	26.02214334272002	27.054328489452224	23.73633962205541
32	22.99653166483672	26.0309127468187	27.176383301812063	23.796172286532514
33	22.744393375874104	25.804891511479678	27.431054443184657	24.019660669461565
34	23.230151457405665	26.056551903761164	26.901329866989403	23.811966771843764
35	22.722410128542258	26.0184874331094	27.39803178251878	23.861070655829565
36	22.559375675775776	25.90137885139922	27.453778430372246	24.085467042452755
37	22.81705756623947	26.092059627168908	27.16400577777087	23.926877028820755
38	22.254979384731918	26.037961722865326	27.83141238779882	23.875646504603935
39	22.19784683650318	26.797960814861632	27.296311473940893	23.707880874694297
40	22.531084192253058	25.91607417434388	27.57091090691648	23.981930726486585
41	22.276651999221027	25.97645642000428	27.83573735276302	23.911154228011675
42	22.34723733902349	25.84061428839392	27.744817509178603	24.067330863403985
43	22.385373494177426	25.890219963894907	27.548091340392666	24.176315201535004
44	22.381908743239258	26.038248460874	27.69855711044551	23.881285685441238
45	22.02372518073455	25.930124336769012	27.859154290138243	24.186996192358194
46	22.583151035661846	25.97351735541535	27.419680502173833	24.02365110674898
47	22.33703424488143	25.91036330900441	27.745868881877083	24.006733564237077
48	22.30329473919386	26.05227472846508	27.55936970206726	24.0850608302738
49	22.704512897834054	25.989216261390368	27.31490165483673	23.991369185938847
50	22.45457293360462	26.058415700817562	27.388187110887564	24.098824254690257
51	22.480451038887647	25.95855918929607	27.41681312208707	24.144176649729214
52	22.76922010845865	26.100637872595133	27.104746589311123	24.02539542963509
53	22.578467648186802	26.110817071903142	27.159871971479127	24.150843308430932
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	880.0
1	873.5
2	867.0
3	847.0
4	827.0
5	781.0
6	735.0
7	727.5
8	751.0
9	782.0
10	822.5
11	863.0
12	998.5
13	1134.0
14	1372.5
15	1611.0
16	2200.0
17	2789.0
18	3810.0
19	4831.0
20	6847.5
21	8864.0
22	12595.0
23	16326.0
24	20372.0
25	30394.0
26	36370.0
27	44042.0
28	51714.0
29	61273.5
30	70833.0
31	82620.5
32	94408.0
33	108463.5
34	122519.0
35	137644.5
36	152770.0
37	169679.0
38	186588.0
39	206174.0
40	225760.0
41	244875.0
42	281155.5
43	298321.0
44	313248.0
45	328175.0
46	336938.5
47	345702.0
48	349195.0
49	352688.0
50	348006.5
51	343325.0
52	329713.0
53	316101.0
54	292938.0
55	269775.0
56	240984.0
57	212193.0
58	184685.5
59	132865.0
60	108552.0
61	90025.5
62	71499.0
63	58325.0
64	45151.0
65	36667.5
66	28184.0
67	22480.0
68	16776.0
69	13124.0
70	9472.0
71	7298.0
72	5124.0
73	3971.5
74	2819.0
75	2173.0
76	1189.0
77	851.0
78	669.5
79	488.0
80	396.0
81	304.0
82	220.0
83	136.0
84	84.0
85	32.0
86	23.5
87	15.0
88	12.0
89	9.0
90	6.5
91	4.0
92	4.5
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	4185005.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.10444553471487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.20151704649867	81.18266465329542
2	4.045504867883139	7.047629168499014
3	1.241098466281563	3.243155812783217
4	0.5567527966703656	1.9398257461549617
5	0.3056344278350575	1.3311058686446255
6	0.18869946791804287	0.9861937515418105
7	0.12350992870554739	0.7530784700550208
8	0.08183187233952012	0.5702335893761177
9	0.0613631071753171	0.48104994841139515
>10	0.1935469513184646	2.3955312692777735
>50	4.581211482263807E-4	0.025141505789963915
>100	5.531780951126396E-5	0.017051718361793974
>500	0.0	0.0
>1k	2.7628416562866748E-5	0.02733849780887288
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1947417028175594E-4	0.0	0.0	0.0	0.0
2	1.1947417028175594E-4	0.0	0.0	0.0	0.0
3	1.1947417028175594E-4	0.0	0.0	0.0	0.0
4	1.1947417028175594E-4	0.0	0.0	4.7789668112702375E-5	0.0
5	1.1947417028175594E-4	0.0	0.0	4.7789668112702375E-5	0.0
6	1.4336900433810712E-4	0.0	0.0	4.7789668112702375E-5	0.0
7	1.4336900433810712E-4	0.0	0.0	4.7789668112702375E-5	0.0
8	1.4336900433810712E-4	0.0	0.0	7.168450216905356E-5	0.0
9	1.672638383944583E-4	0.0	0.0	7.168450216905356E-5	0.0
10	1.672638383944583E-4	0.0	0.0	9.557933622540475E-5	0.0
11	1.911586724508095E-4	0.0	0.0	9.557933622540475E-5	0.0
12	1.911586724508095E-4	0.0	0.0	9.557933622540475E-5	0.0
13	2.150535065071607E-4	0.0	0.0	9.557933622540475E-5	0.0
14	2.150535065071607E-4	0.0	0.0	9.557933622540475E-5	0.0
15	2.3894834056351188E-4	0.0	0.0	9.557933622540475E-5	4.7789668112702375E-5
16	2.8673800867621424E-4	0.0	0.0	1.1947417028175594E-4	4.7789668112702375E-5
17	3.1063284273256546E-4	0.0	0.0	1.1947417028175594E-4	4.7789668112702375E-5
18	4.5400184707067255E-4	0.0	0.0	1.4336900433810712E-4	4.7789668112702375E-5
19	4.5400184707067255E-4	0.0	0.0	1.4336900433810712E-4	4.7789668112702375E-5
20	5.495811832960773E-4	0.0	0.0	1.4336900433810712E-4	4.7789668112702375E-5
21	6.690553535778332E-4	0.0	0.0	1.672638383944583E-4	4.7789668112702375E-5
22	6.929501876341845E-4	0.0	0.0	1.672638383944583E-4	4.7789668112702375E-5
23	7.64634689803238E-4	0.0	0.0	1.672638383944583E-4	4.7789668112702375E-5
24	9.080036941413451E-4	0.0	0.0	1.911586724508095E-4	4.7789668112702375E-5
25	0.0010035830303667499	0.0	0.0	1.911586724508095E-4	4.7789668112702375E-5
26	0.0010752675325358034	0.0	0.0	2.150535065071607E-4	4.7789668112702375E-5
27	0.001266426204986613	0.0	0.0	2.3894834056351188E-4	4.7789668112702375E-5
28	0.00140979520932472	0.0	0.0	3.1063284273256546E-4	4.7789668112702375E-5
29	0.0014814797114937736	0.0	0.0	3.584225108452678E-4	4.7789668112702375E-5
30	0.0015531642136628271	0.0	0.0	3.82317344901619E-4	4.7789668112702375E-5
31	0.001672638383944583	0.0	0.0	4.5400184707067255E-4	4.7789668112702375E-5
32	0.0017682177201699878	0.0	0.0	5.256863492397261E-4	4.7789668112702375E-5
33	0.0019354815585644462	0.0	0.0	5.495811832960773E-4	4.7789668112702375E-5
34	0.0021266402310152555	0.0	0.0	6.212656854651309E-4	4.7789668112702375E-5
35	0.002293904069409714	0.0	0.0	6.929501876341845E-4	4.7789668112702375E-5
36	0.0027479059164803865	0.0	0.0	7.168450216905356E-4	4.7789668112702375E-5
37	0.002939064588931196	0.0	0.0	7.407398557468868E-4	4.7789668112702375E-5
38	0.0030824335932693033	0.0	0.0	8.602140260286428E-4	4.7789668112702375E-5
39	0.003178012929494708	0.0	0.0	9.080036941413451E-4	4.7789668112702375E-5
40	0.0032258025976074104	0.0	0.0	9.796881963103988E-4	4.7789668112702375E-5
41	0.0032735922657201127	0.0	0.0	0.0010513726984794523	4.7789668112702375E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	2545	0.0	31.764244	12
CAACGCA	2630	0.0	31.095057	13
ATCAACG	2585	0.0	30.999998	11
GGTATCA	2595	0.0	29.25048	8
GTATCAA	2690	0.0	29.003717	9
AACGCAG	2720	0.0	28.856619	14
GATCGGA	540	0.0	28.722223	1
AGTGGTA	2670	0.0	27.460672	5
TGGTATC	3090	0.0	24.944984	7
TATCAAC	3330	0.0	24.840843	10
GTGGTAT	3025	0.0	24.393387	6
AAGCAGT	2900	0.0	24.148275	1
GCAGTGG	3060	0.0	22.96242	3
TATGCCG	675	0.0	22.28148	16
ACGCAGA	3680	0.0	21.775816	15
CAGTGGT	3440	0.0	21.58721	4
AGAGTAC	3655	0.0	21.281807	19
ACATGGG	1770	0.0	20.977402	24
CGCAGAG	4160	0.0	19.432693	16
GCCGTCT	835	0.0	18.57485	19
>>END_MODULE