FastQCFastQC Report
Thu 26 May 2016
SRR805265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41014168
Sequences flagged as poor quality0
Sequence length53
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGT4753021.1588727095475886No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2822850.6882621634553211No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGT2199360.5362439633055582No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1522910.3713131520795448No Hit
AGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACAT1129030.27527804538178124No Hit
AGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTA910930.22210129923883865No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGA722110.17606354955194994No Hit
AACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAG668310.1629461312003208No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT658060.16044699480433203No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA532440.1298185544078329No Hit
AGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTACAT529360.1290675943981114No Hit
AGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA503940.12286973613605913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCACCGCAGAGT478380.11663774332811043No Hit
AGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTA424740.10355933588607723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGA415900.10140398313090246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTTT385950.034.8648822
TACTTTT396900.033.66024423
GAGTACT448650.032.23949420
ACTTTTT425950.031.3590824
AGTACTT459300.030.89331421
CTTTTTT472200.028.3025225
GCAGAGA604150.026.29090347
CCGCAGA475350.022.90428245
CATGGGG602250.022.49912625
ACCGCAG578300.019.18035744
ATCACCG607250.018.13824541
CACCGCA628400.017.841943
ATGGGGA372400.017.51772126
TCACCGC658350.017.02308742
TATCACC666900.016.56874840
ATGGGGC129350.015.5879426
ATGGGGG289800.015.32608726
TCCGATC17950.014.53203245
GTATCAC736300.014.29532839
CATGGGT174200.014.28616425
Produced by FastQC (version 0.11.5)