##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805265_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41014168
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.48042030256472	35.0	33.0	35.0	31.0	35.0
2	34.526305031958714	35.0	35.0	36.0	32.0	37.0
3	37.04567114466396	39.0	37.0	39.0	34.0	39.0
4	37.02662384861738	39.0	37.0	39.0	33.0	39.0
5	36.97239648991539	39.0	37.0	39.0	33.0	39.0
6	36.99883910847588	38.0	37.0	39.0	33.0	39.0
7	36.91721368089193	38.0	37.0	39.0	33.0	39.0
8	37.893631707950284	40.0	38.0	40.0	33.0	40.0
9	37.81784662802376	40.0	37.0	40.0	33.0	40.0
10	37.50330785693373	39.0	37.0	40.0	33.0	40.0
11	37.23881289021882	39.0	36.0	40.0	32.0	40.0
12	37.0700181459246	38.0	36.0	40.0	32.0	40.0
13	37.13412494433631	38.0	36.0	40.0	32.0	40.0
14	36.909414449172786	38.0	36.0	40.0	32.0	40.0
15	36.68602415633544	38.0	36.0	40.0	32.0	40.0
16	36.44774025405075	38.0	35.0	40.0	31.0	40.0
17	36.055451935536034	38.0	34.0	40.0	31.0	40.0
18	35.85245961834457	38.0	34.0	39.0	30.0	40.0
19	35.5571549080308	37.0	34.0	39.0	29.0	40.0
20	35.133107247232225	37.0	33.0	39.0	29.0	40.0
21	34.60279223511251	36.0	33.0	38.0	27.0	40.0
22	34.48539524195639	36.0	32.0	38.0	27.0	40.0
23	33.91585831998348	36.0	32.0	38.0	27.0	40.0
24	33.283978916748964	35.0	31.0	38.0	25.0	39.0
25	32.83907426818947	34.0	30.0	38.0	25.0	39.0
26	32.80661799600567	34.0	30.0	38.0	25.0	39.0
27	32.278204034274204	34.0	30.0	37.0	24.0	39.0
28	31.78744849828479	34.0	29.0	37.0	24.0	38.0
29	31.21313537312277	33.0	29.0	37.0	21.0	38.0
30	30.778314410766544	33.0	27.0	36.0	19.0	38.0
31	30.482379674262805	32.0	27.0	36.0	19.0	38.0
32	30.041523114646626	32.0	26.0	36.0	18.0	38.0
33	29.65362959453426	31.0	26.0	35.0	18.0	38.0
34	28.98597862572758	31.0	25.0	35.0	16.0	38.0
35	28.575863613763907	30.0	24.0	35.0	15.0	38.0
36	28.080626723916478	30.0	24.0	34.0	15.0	37.0
37	27.100694228394442	30.0	23.0	34.0	10.0	37.0
38	27.097204092985624	30.0	23.0	34.0	14.0	37.0
39	26.4570393820984	29.0	20.0	33.0	10.0	37.0
40	25.927122134965654	28.0	19.0	33.0	10.0	36.0
41	25.14124892159217	27.0	19.0	33.0	10.0	36.0
42	25.062443421990178	27.0	19.0	33.0	10.0	35.0
43	24.365249832691962	26.0	18.0	32.0	10.0	35.0
44	23.74167746131044	25.0	15.0	31.0	10.0	35.0
45	23.1826580268555	24.0	15.0	31.0	9.0	35.0
46	22.8027724224468	24.0	14.0	31.0	9.0	34.0
47	21.83548031499749	23.0	13.0	30.0	9.0	34.0
48	22.038439155952158	23.0	13.0	30.0	9.0	33.0
49	21.16341492042457	23.0	12.0	29.0	9.0	33.0
50	20.808051403115137	22.0	12.0	28.0	9.0	33.0
51	20.40898513411268	22.0	10.0	28.0	9.0	32.0
52	20.37690668258832	22.0	10.0	28.0	9.0	32.0
53	18.459943110390537	18.0	9.0	26.0	9.0	31.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	10.0
12	72.0
13	272.0
14	960.0
15	3162.0
16	9202.0
17	24192.0
18	57146.0
19	117468.0
20	217018.0
21	358125.0
22	543592.0
23	777461.0
24	1075919.0
25	1488027.0
26	2191747.0
27	3615819.0
28	5127027.0
29	4365300.0
30	2484509.0
31	1607347.0
32	1666531.0
33	2434209.0
34	4232457.0
35	5121101.0
36	2709978.0
37	655213.0
38	123901.0
39	6400.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.577540619621981	45.68716839507753	18.40967735832164	21.325613626978853
2	28.784526361719685	39.51235338968719	19.10265740365622	12.600462844936903
3	29.87159461579228	24.019285238213293	22.906338121987506	23.202782024006925
4	24.03255626202146	26.351545153860002	20.616636670528095	28.999261913590445
5	21.35311388006213	33.30377931840529	22.845659090292898	22.497447711239687
6	26.192968244534427	26.086773234068772	24.757583769589083	22.962674751807715
7	25.158613482053322	23.7757133096056	29.03827526136822	22.02739794697286
8	27.906571212172338	25.25797183061229	24.72527786007996	22.11017909713541
9	30.28380583021945	22.587202061492505	24.37035172821255	22.75864038007549
10	23.19507249299803	23.84978527420086	32.34023910956819	20.614903123232928
11	22.706651028493376	34.15231292757176	23.390324533707474	19.750711510227394
12	21.73604253047386	22.594748234317468	30.709629413913746	24.959579821294923
13	23.198385982131832	26.27866058382557	23.87338199814269	26.649571435899905
14	21.228386249356564	32.720880745404855	24.02866492378926	22.022068081449316
15	22.48063644738569	32.47088664580493	23.625823642210662	21.42265326459871
16	25.409838863487366	23.264648450262357	22.92581919496697	28.39969349128331
17	28.2150694852569	24.957124572172233	24.122773866825728	22.705032075745144
18	23.602175716449985	25.637433386433685	22.87999112891916	27.880399768197172
19	22.324478214455063	31.8439983958714	24.24598007205705	21.585543317616487
20	30.99310218849252	25.490759680898563	22.98280681934106	20.53333131126785
21	24.055214285951138	30.12047934265057	22.417246157474167	23.407060213924126
22	28.16839537010723	26.250826299828876	24.104065697492633	21.476712632571264
23	23.16322252349481	24.847618510754625	31.36564418422434	20.62351478152623
24	22.107543422555835	30.411788433694426	25.491427742725392	21.98924040102435
25	23.172653410889623	24.383337484744295	21.915714589163432	30.528294515202646
26	23.771634231370975	30.15040802485619	24.29347829267194	21.784479451100896
27	22.035595114351704	24.900795256897567	32.59675778379803	20.4668518449527
28	28.683041918587744	24.720240576378387	23.871265656297112	22.725451848736757
29	31.043896830968265	23.216174956907572	25.70519777458365	20.03473043754051
30	25.13501675811149	24.242408135647175	26.013357140391097	24.609217965850238
31	20.98387562073672	33.1433835254198	23.873445390870785	21.999295462972697
32	25.11126642871312	31.22680923333615	24.05743059325256	19.604493744698175
33	25.937103003040313	23.870553707196986	25.30265882755442	24.88968446220828
34	24.494328398908397	25.282346334564192	23.8981685548272	26.32515671170021
35	23.013001263368306	29.69494590259639	24.005953259858888	23.286099574176415
36	24.841525494312112	26.435060196759324	25.92421721196441	22.799197096964154
37	24.71380865265876	24.311311154720975	28.611810435847435	22.36306975677283
38	27.080627357843756	25.317902340479026	25.11375337419986	22.48771692747735
39	28.01280767172944	23.263005115695627	25.245444452268302	23.478742760306634
40	23.309620714480907	24.183469965793282	30.984539293836217	21.52237002588959
41	22.71766673409052	31.40650811202607	25.052318506131833	20.82350664775158
42	21.82780106620717	22.903838985591516	29.825576371560185	25.442783576641126
43	23.228421944338844	25.98604462731025	25.423446356390794	25.36208707196011
44	21.47240680342461	29.997785155607694	25.487036577214	23.0427714637537
45	22.641892918564142	29.11863285877212	25.207950091782916	23.031524130880822
46	24.757230233220874	23.439134496157525	25.029289878560988	26.774345392060617
47	25.512259568449615	25.335957077076387	25.665591948616388	23.486191405857603
48	23.48872223861764	25.732944284033753	24.69515656150821	26.0831769158404
49	22.101018847925914	29.276283258994795	26.28543629118601	22.337261601893278
50	27.902475066664767	25.809647046844887	24.86595363826471	21.421924248225636
51	23.56890916329206	27.923209365115003	24.423465081627402	24.084416389965536
52	25.56103051999007	26.248407623433934	25.97494846171206	22.21561339486394
53	22.814933122622406	25.790953506602886	29.630302387214098	21.763810983560607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9569.0
1	6367.0
2	3165.0
3	2611.0
4	2057.0
5	1882.5
6	1708.0
7	1742.0
8	1962.0
9	2148.0
10	2817.5
11	3487.0
12	4860.5
13	6234.0
14	8467.0
15	10700.0
16	15500.5
17	20301.0
18	29761.5
19	39222.0
20	54103.0
21	68984.0
22	177795.5
23	286607.0
24	254341.0
25	247986.0
26	273897.0
27	312718.0
28	351539.0
29	402015.0
30	452491.0
31	512503.0
32	572515.0
33	650325.5
34	728136.0
35	804672.5
36	881209.0
37	977111.0
38	1073013.0
39	1198282.0
40	1323551.0
41	1508711.0
42	2128715.5
43	2563560.0
44	3572055.5
45	4580551.0
46	5659848.5
47	6739146.0
48	6190773.0
49	5642400.0
50	4598547.5
51	3554695.0
52	2986127.5
53	2417560.0
54	2165483.5
55	1913407.0
56	1710553.5
57	1507700.0
58	1353142.0
59	1072838.5
60	947093.0
61	830447.0
62	713801.0
63	603747.5
64	493694.0
65	411472.5
66	329251.0
67	258227.5
68	187204.0
69	144276.5
70	101349.0
71	75852.5
72	50356.0
73	36659.0
74	22962.0
75	16730.5
76	7968.5
77	5438.0
78	4300.0
79	3162.0
80	2555.5
81	1949.0
82	1433.0
83	917.0
84	635.0
85	353.0
86	245.5
87	138.0
88	101.5
89	65.0
90	51.0
91	37.0
92	29.0
93	16.0
94	11.0
95	8.5
96	6.0
97	5.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	4.1014168E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.593089045940324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.33310697228943	38.90599944754343
2	7.329461603296022	6.683455910757521
3	2.5044626011396405	3.4255855915796087
4	1.24827625191313	2.276510812296319
5	0.7340903012622774	1.6734722236606085
6	0.49871203420833327	1.3642693310365543
7	0.3431479348195166	1.0951622043709723
8	0.25033339226310464	0.9130778115699228
9	0.20948029794858533	0.8595768489965966
>10	1.306437420580839	11.574625375342924
>50	0.13768951993314765	4.372922235581135
>100	0.09241866954111604	7.927760398154983
>500	0.006329906121730237	1.9824934707356725
>1k	0.004301397524486738	4.271670644581909
>5k	0.001056326480159618	3.369639797410956
>10k+	6.953706785075494E-4	9.303777896380968
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGT	475302	1.1588727095475886	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	282285	0.6882621634553211	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGT	219936	0.5362439633055582	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	152291	0.3713131520795448	No Hit
AGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACAT	112903	0.27527804538178124	No Hit
AGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTA	91093	0.22210129923883865	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGA	72211	0.17606354955194994	No Hit
AACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAG	66831	0.1629461312003208	No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	65806	0.16044699480433203	No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA	53244	0.1298185544078329	No Hit
AGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTACAT	52936	0.1290675943981114	No Hit
AGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA	50394	0.12286973613605913	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCACCGCAGAGT	47838	0.11663774332811043	No Hit
AGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTA	42474	0.10355933588607723	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGA	41590	0.10140398313090246	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7067272948216334E-5	0.0	0.0	0.0	2.4381818497451903E-6
2	1.7067272948216334E-5	0.0	0.0	0.0	2.4381818497451903E-6
3	1.7067272948216334E-5	0.0	0.0	0.0	2.4381818497451903E-6
4	2.1943636647706714E-5	0.0	0.0	2.4381818497451903E-6	2.4381818497451903E-6
5	2.1943636647706714E-5	0.0	0.0	2.4381818497451903E-6	2.4381818497451903E-6
6	2.1943636647706714E-5	0.0	0.0	2.4381818497451903E-6	2.4381818497451903E-6
7	2.6820000347197095E-5	0.0	0.0	2.4381818497451903E-6	2.4381818497451903E-6
8	2.6820000347197095E-5	0.0	0.0	2.4381818497451903E-6	2.4381818497451903E-6
9	3.6572727746177856E-5	0.0	2.4381818497451903E-6	2.4381818497451903E-6	2.4381818497451903E-6
10	6.583090994312015E-5	0.0	4.8763636994903806E-6	4.8763636994903806E-6	2.4381818497451903E-6
11	1.7067272948216334E-4	0.0	4.8763636994903806E-6	4.8763636994903806E-6	4.8763636994903806E-6
12	2.486945486740094E-4	0.0	4.8763636994903806E-6	9.752727398980761E-6	4.8763636994903806E-6
13	4.1936727815617276E-4	0.0	4.8763636994903806E-6	9.752727398980761E-6	7.314545549235572E-6
14	6.948818271773793E-4	0.0	4.8763636994903806E-6	1.2190909248725953E-5	9.752727398980761E-6
15	0.0011556981967792202	0.0	4.8763636994903806E-6	1.7067272948216334E-5	9.752727398980761E-6
16	0.001548245474588196	0.0	4.8763636994903806E-6	1.7067272948216334E-5	1.2190909248725953E-5
17	0.00217485820997271	0.0	4.8763636994903806E-6	1.7067272948216334E-5	1.4629091098471143E-5
18	0.0027575836720618104	0.0	4.8763636994903806E-6	1.7067272948216334E-5	1.4629091098471143E-5
19	0.0033622527707986177	0.0	4.8763636994903806E-6	1.9505454797961522E-5	1.4629091098471143E-5
20	0.004118089144219627	0.0	4.8763636994903806E-6	2.6820000347197095E-5	1.7067272948216334E-5
21	0.005200641885506492	2.4381818497451903E-6	4.8763636994903806E-6	2.6820000347197095E-5	1.7067272948216334E-5
22	0.006239307353497942	2.4381818497451903E-6	4.8763636994903806E-6	2.6820000347197095E-5	2.1943636647706714E-5
23	0.007870451010977475	2.4381818497451903E-6	4.8763636994903806E-6	2.6820000347197095E-5	2.4381818497451906E-5
24	0.009713716489384839	4.8763636994903806E-6	4.8763636994903806E-6	2.6820000347197095E-5	2.4381818497451906E-5
25	0.01169595833322768	4.8763636994903806E-6	4.8763636994903806E-6	2.9258182196942287E-5	2.4381818497451906E-5
26	0.014119511091874398	4.8763636994903806E-6	4.8763636994903806E-6	3.169636404668748E-5	2.6820000347197095E-5
27	0.017167238404055887	4.8763636994903806E-6	4.8763636994903806E-6	3.169636404668748E-5	2.9258182196942287E-5
28	0.020561187538901193	4.8763636994903806E-6	4.8763636994903806E-6	3.413454589643267E-5	2.9258182196942287E-5
29	0.02445008758924477	4.8763636994903806E-6	4.8763636994903806E-6	3.6572727746177856E-5	2.9258182196942287E-5
30	0.028924151283527193	7.314545549235572E-6	4.8763636994903806E-6	4.144909144566824E-5	2.9258182196942287E-5
31	0.0329008258804616	7.314545549235572E-6	4.8763636994903806E-6	4.388727329541343E-5	2.9258182196942287E-5
32	0.03755287684977542	7.314545549235572E-6	4.8763636994903806E-6	4.876363699490381E-5	2.9258182196942287E-5
33	0.04182944781422849	9.752727398980761E-6	4.8763636994903806E-6	5.607818254413938E-5	3.169636404668748E-5
34	0.0473494915220516	9.752727398980761E-6	4.8763636994903806E-6	6.583090994312015E-5	3.169636404668748E-5
35	0.052628155226749934	9.752727398980761E-6	4.8763636994903806E-6	7.802181919184609E-5	3.413454589643267E-5
36	0.058413960756195274	1.2190909248725953E-5	4.8763636994903806E-6	8.046000104159129E-5	3.413454589643267E-5
37	0.06470446992853787	1.2190909248725953E-5	4.8763636994903806E-6	9.265091029031723E-5	3.413454589643267E-5
38	0.0719629372952293	1.2190909248725953E-5	4.8763636994903806E-6	1.0971818323853357E-4	3.6572727746177856E-5
39	0.08009915012782899	1.2190909248725953E-5	4.8763636994903806E-6	1.1459454693802396E-4	3.6572727746177856E-5
40	0.08842066478100934	1.2190909248725953E-5	4.8763636994903806E-6	1.316618198862403E-4	3.9010909595923044E-5
41	0.09749557762576093	1.2190909248725953E-5	4.8763636994903806E-6	1.4141454728522105E-4	3.9010909595923044E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTTT	38595	0.0	34.86488	22
TACTTTT	39690	0.0	33.660244	23
GAGTACT	44865	0.0	32.239494	20
ACTTTTT	42595	0.0	31.35908	24
AGTACTT	45930	0.0	30.893314	21
CTTTTTT	47220	0.0	28.30252	25
GCAGAGA	60415	0.0	26.290903	47
CCGCAGA	47535	0.0	22.904282	45
CATGGGG	60225	0.0	22.499126	25
ACCGCAG	57830	0.0	19.180357	44
ATCACCG	60725	0.0	18.138245	41
CACCGCA	62840	0.0	17.8419	43
ATGGGGA	37240	0.0	17.517721	26
TCACCGC	65835	0.0	17.023087	42
TATCACC	66690	0.0	16.568748	40
ATGGGGC	12935	0.0	15.58794	26
ATGGGGG	28980	0.0	15.326087	26
TCCGATC	1795	0.0	14.532032	45
GTATCAC	73630	0.0	14.295328	39
CATGGGT	17420	0.0	14.286164	25
>>END_MODULE