FastQCFastQC Report
Thu 26 May 2016
SRR805264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18148223
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT8225904.532620080764932No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6614883.6449188441204408No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG1270040.6998150728035467No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG1057250.5825639237516533No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA946550.5215662161524024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC750000.4132636016209411No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG740080.40779750171683476No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC635580.35021610655765034No Hit
TAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG616540.3397247212578333No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT600620.3309525125407595No Hit
AGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG555340.30600241136556455No Hit
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT537860.29637061435711914No Hit
TGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG515600.28410495066100966No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGT506310.2789859921822649No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA500640.2758617193540106No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA450860.24843203656909No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA446710.24614531130678746No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG438460.24159941168895707No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT433780.23902064681484242No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG428970.23637024958311345No Hit
TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTG412140.22709661436273954No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCG402030.22152582101288926No Hit
TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC386570.21300708063814291No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA382710.2108801506351338No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC371760.2048465020514681No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT370000.2038767101329976No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC365620.2014632506995313No Hit
TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT362200.1995787686761398No Hit
CTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC333550.18379209909421987No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA332750.18335128458582417No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC326850.18010027758640612No Hit
ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG303380.16716788194634816No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC300970.16583992823980617No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT292800.1613381100728154No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA288600.15902383390373812No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT283480.15620262105000585No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA283240.15607037669748713No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG271470.14958489324271582No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACT269980.1487638762208289No Hit
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG267330.14730367816176823No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC265430.1462567437043285No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC263240.14505001398759537No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA245170.13509311627920817No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG240210.132360066327155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTCAGT239550.13199639435772856No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA237550.1308943580867394No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT234810.12938456839548423No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA221850.12224337335947437No Hit
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC218100.12017705535136967No Hit
TCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT210380.11592319534535145No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT209550.11546585029289093No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA202080.11134974482074635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC3572700.044.5281219
GAGTACT994950.044.5128920
GTACTTT990800.044.30323822
GTACATG2569950.044.08027622
GAGTACA2594550.044.07444420
ACATGGG2543850.044.05216624
AGTACAT2579500.044.0418921
TACATGG2569300.044.00727523
AGTACTT1006150.043.8118621
TACTTTT1003950.043.5895223
CATGGGG1685850.043.57365825
ATGGGGG867300.043.13345726
ACTTTTT1018050.043.08968424
TGGGGGG587400.042.19518327
CATGGGC373750.041.02675625
ATGGGGC279400.040.7170726
CCGATCC91850.040.60370346
CTTTTTT1082550.040.47240425
CGATCCC92400.040.38744747
CATGGGA364100.040.3069225
Produced by FastQC (version 0.11.5)