FastQCFastQC Report
Thu 26 May 2016
SRR805263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18720561
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1797440.9601421666797273No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC254240.13580789592790515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1044650.043.2679821
CAACGCA1130000.039.98951313
TCAACGC1130250.039.9848212
AGCAGTG1136950.039.790532
GTGGTAT1140050.039.7709736
AACGCAG1138950.039.73097614
AGTGGTA1140550.039.7082065
GTATCAA1140250.039.7021689
ACGCAGA1140050.039.66584415
GGTATCA1143450.039.5931138
ATCAACG1142500.039.58695611
TGGTATC1149700.039.384017
TATCAAC1150000.039.3349110
AGAGTAC1140350.039.2473819
CGCAGAG1157900.039.07465716
CAGTGGT1171450.038.6447564
GCAGTGG1176500.038.4888653
CAGAGTA1172650.038.29859518
GCAGAGT1192250.037.81681417
ACATGGG853800.037.7877124
Produced by FastQC (version 0.11.5)