##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805263_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18720561
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.58204030317254	37.0	37.0	37.0	35.0	37.0
2	36.48633200682394	37.0	37.0	37.0	35.0	37.0
3	38.31029919455939	39.0	39.0	39.0	37.0	39.0
4	38.237180338772966	39.0	39.0	39.0	37.0	39.0
5	38.15838953757849	39.0	39.0	39.0	37.0	39.0
6	38.170091537320914	39.0	39.0	39.0	37.0	39.0
7	38.220663472638456	39.0	39.0	39.0	37.0	39.0
8	39.172761916696835	40.0	40.0	40.0	38.0	40.0
9	39.16749957439844	40.0	40.0	40.0	38.0	40.0
10	39.13541282229737	40.0	40.0	40.0	38.0	40.0
11	39.133635525131965	40.0	40.0	40.0	38.0	40.0
12	39.127115688466816	40.0	40.0	40.0	38.0	40.0
13	39.160757041415586	40.0	40.0	40.0	38.0	40.0
14	39.19903287086322	40.0	40.0	40.0	38.0	40.0
15	39.17933153819482	40.0	40.0	40.0	38.0	40.0
16	39.198040218987025	40.0	40.0	40.0	38.0	40.0
17	39.163681579841544	40.0	40.0	40.0	38.0	40.0
18	39.11263615444003	40.0	40.0	40.0	38.0	40.0
19	39.10510480962616	40.0	40.0	40.0	38.0	40.0
20	39.0933814430027	40.0	40.0	40.0	38.0	40.0
21	39.062889568320095	40.0	40.0	40.0	38.0	40.0
22	39.04191268627046	40.0	40.0	40.0	37.0	40.0
23	38.94737871370415	40.0	40.0	40.0	37.0	40.0
24	38.95607898716283	40.0	40.0	40.0	37.0	40.0
25	38.94909938863477	40.0	40.0	40.0	37.0	40.0
26	38.89345057554632	40.0	40.0	40.0	37.0	40.0
27	38.84611027415257	40.0	40.0	40.0	37.0	40.0
28	38.82850887855337	40.0	40.0	40.0	37.0	40.0
29	38.79840395808651	40.0	40.0	40.0	37.0	40.0
30	38.74546259591259	40.0	40.0	40.0	36.0	40.0
31	38.69170026475168	40.0	39.0	40.0	36.0	40.0
32	38.65603958129246	40.0	39.0	40.0	36.0	40.0
33	38.612163705991506	40.0	39.0	40.0	35.0	40.0
34	38.547582788785014	40.0	39.0	40.0	35.0	40.0
35	38.50938227759307	40.0	39.0	40.0	35.0	40.0
36	38.457203819906894	40.0	39.0	40.0	35.0	40.0
37	38.36813875396149	40.0	38.0	40.0	35.0	40.0
38	38.28025618463037	40.0	38.0	40.0	35.0	40.0
39	38.202073378036054	40.0	38.0	40.0	35.0	40.0
40	38.13119200861556	40.0	38.0	40.0	35.0	40.0
41	38.07565040385275	40.0	38.0	40.0	34.0	40.0
42	37.9805621209749	40.0	38.0	40.0	34.0	40.0
43	38.062426654842234	40.0	38.0	40.0	35.0	40.0
44	38.073039798326555	40.0	38.0	40.0	35.0	40.0
45	38.03156192808538	40.0	38.0	40.0	35.0	40.0
46	37.99383960769124	40.0	38.0	40.0	34.0	40.0
47	38.040778852727755	40.0	38.0	40.0	35.0	40.0
48	38.033901975480326	40.0	38.0	40.0	35.0	40.0
49	37.98028002472789	40.0	38.0	40.0	35.0	40.0
50	37.91540590049625	40.0	37.0	40.0	35.0	40.0
51	37.76259557606206	40.0	37.0	40.0	34.0	40.0
52	37.61024106061779	40.0	37.0	40.0	34.0	40.0
53	37.29937494928704	40.0	36.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	25.0
13	41.0
14	175.0
15	469.0
16	1151.0
17	2049.0
18	3579.0
19	5169.0
20	7834.0
21	11329.0
22	15419.0
23	20211.0
24	26098.0
25	33595.0
26	43040.0
27	54103.0
28	68302.0
29	86178.0
30	107609.0
31	135255.0
32	170524.0
33	218906.0
34	292225.0
35	433427.0
36	696390.0
37	1276498.0
38	2749467.0
39	1.2261491E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.52193606804839	29.953674999376357	23.953213795248978	21.57117513732628
2	24.54336704973745	26.96083733815456	26.213642849698786	22.282152762409204
3	26.830616881620163	24.086676675982094	24.83555914804049	24.24714729435726
4	22.986848524464627	26.063177273373377	24.350910210436535	26.599063991725462
5	24.868757939465596	30.134257194535998	23.259110664472075	21.737874201526335
6	28.53822062276873	24.33849071082859	25.15590211212153	21.967386554281145
7	23.15767139670654	24.833059222958116	29.852075480002977	22.157193900332366
8	27.87382814008619	24.429193120868547	25.04244931548793	22.65452942355734
9	27.403826199439212	24.692945900499456	25.399623440771883	22.50360445928944
10	22.188507064505174	24.982846400810317	30.57650889842457	22.25213763625994
11	22.190793320777086	29.894574206403323	25.488947686984382	22.425684785835212
12	22.148096950727066	24.75568440497056	30.445556626214355	22.650662018088028
13	22.294636362660285	25.009026171811836	25.153156467907134	27.54318099762074
14	22.369383054279197	29.92705720731339	25.162290809554268	22.541268928853146
15	22.51563935503856	29.609310319279423	25.093094165287035	22.781956160394977
16	22.608286151253694	24.63670826958658	25.04696841082914	27.708037168330584
17	27.63446031344894	24.616340290229548	25.08313185699937	22.66606753932214
18	22.5693717191488	24.624288769978634	25.05335176654161	27.752987744330955
19	22.85865257990933	29.75916693949503	24.909034510237166	22.473145970358473
20	27.81643135587657	24.62928862014338	25.05266802634814	22.50161199763191
21	22.746829007955476	29.489773303267995	25.131223364513488	22.632174324263037
22	27.75318004626037	24.55256015030746	25.135694384372346	22.55856541905982
23	22.65053381680175	24.654902168797186	30.05505550821901	22.63950850618205
24	22.515003690327443	29.645986570594758	24.96111094106635	22.877898798011447
25	22.706723372232275	24.604983792953643	25.025200900763604	27.66309193405048
26	22.912027048762056	28.09830859235468	26.486353694208205	22.503310664675062
27	23.057385940517488	24.33573972489393	30.015484044521955	22.59139029006663
28	26.87067978358127	24.42818887745939	26.240618537019273	22.46051280194007
29	26.773551284066755	24.418183835409632	26.264196890253448	22.544067990270165
30	26.531400421173274	24.412478878170372	26.31767285179114	22.738447848865214
31	25.14230743405606	25.12865933878798	26.537009227447832	23.192023999708127
32	24.0974509257495	25.7171139262333	26.89688092146384	23.28855422655336
33	23.959303356347068	25.33061375671381	27.049306909125214	23.660775977813913
34	23.812267164429528	25.344368686387124	27.22845752325478	23.614906625928572
35	23.712558614028715	25.40066507622288	27.209943120828484	23.676833188919925
36	23.661774879502808	25.424024418926333	27.16993897779025	23.744261723780607
37	24.001454870930417	25.344187067898233	27.081645683588224	23.57271237758313
38	24.003490066350043	25.371718294125905	27.110565757083883	23.51422588244017
39	24.04783702796086	25.295593438679536	27.028832095362954	23.62773743799665
40	23.9568995822294	25.480641311977774	26.847715728177164	23.71474337761566
41	23.835749366698998	25.683845692444795	26.92317821031111	23.557226730545093
42	23.909587965873456	25.58755050129107	26.974896745882777	23.527964786952698
43	24.023259773037783	25.41733658515896	27.020541745517136	23.53886189628612
44	23.96702748384517	25.302842153074366	26.971723764047457	23.75840659903301
45	23.872692704027408	25.17422955433868	26.988587574912952	23.964490166720964
46	23.979986497199523	25.356237988808132	26.810713631925882	23.853061882066463
47	23.944111503923413	25.346686992980604	26.844590821824198	23.864610681271785
48	23.941884006574377	25.298237590208966	26.88087712756044	23.87900127565622
49	23.848713721773613	25.666116522896935	26.711833048165595	23.77333670716385
50	23.973838177178557	25.531157960490606	26.828362675669815	23.666641186661018
51	23.879930734981713	25.39551565789081	26.8031390725951	23.921414534532378
52	24.01809432954493	25.49113779229159	26.64416947761341	23.84659840055007
53	24.01412543139065	25.541061509855396	26.544231233241355	23.900581825512603
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6725.0
1	4367.5
2	2010.0
3	1854.5
4	1699.0
5	1701.5
6	1704.0
7	1945.0
8	2504.5
9	2823.0
10	3706.0
11	4589.0
12	6224.5
13	7860.0
14	10100.5
15	12341.0
16	17416.0
17	22491.0
18	30642.5
19	38794.0
20	51478.0
21	64162.0
22	187548.0
23	310934.0
24	240864.0
25	188390.5
26	205987.0
27	232277.5
28	258568.0
29	288967.0
30	319366.0
31	347457.5
32	375549.0
33	418521.0
34	461493.0
35	515336.5
36	569180.0
37	629532.0
38	689884.0
39	749001.0
40	808118.0
41	873971.0
42	1006350.5
43	1072877.0
44	1139831.0
45	1206785.0
46	1261827.0
47	1316869.0
48	1361987.0
49	1407105.0
50	1417000.0
51	1426895.0
52	1402223.0
53	1377551.0
54	1339455.0
55	1301359.0
56	1226057.0
57	1150755.0
58	1041100.5
59	823282.0
60	715118.0
61	631513.0
62	547908.0
63	474691.5
64	401475.0
65	333684.5
66	265894.0
67	213556.5
68	161219.0
69	127709.5
70	94200.0
71	66463.0
72	38726.0
73	27208.5
74	15691.0
75	11724.5
76	5188.0
77	2618.0
78	1642.0
79	666.0
80	460.0
81	254.0
82	195.0
83	136.0
84	96.0
85	56.0
86	54.0
87	52.0
88	37.5
89	23.0
90	17.5
91	12.0
92	11.5
93	10.0
94	9.0
95	8.5
96	8.0
97	5.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8720561E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.03632625792022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.29901776684677	32.56082152274882
2	13.891196235119704	12.512168915152882
3	4.872675815156854	6.583422532814444
4	2.380124568126607	4.287682663385653
5	1.4622760154552237	3.292776985558653
6	0.9387300979601945	2.5366172975918833
7	0.6582796132069825	2.0752546800529035
8	0.4740797869657428	1.7080649566459605
9	0.37304344806331474	1.5120455791823064
>10	2.4427370016781085	21.288172090039346
>50	0.1613756182958824	4.797167901783771
>100	0.040536388762511505	3.1535110349209248
>500	0.004454017477755504	1.3808839401558965
>1k	0.0013667658375935807	0.9201109546415374
>5k	7.12406976047373E-5	0.2211616897261316
>10k+	3.5620348802366786E-5	1.170137255598996
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	179744	0.9601421666797273	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	25424	0.13580789592790515	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6323697778074067E-4	0.0	0.0	5.34172026148148E-6	1.068344052296296E-5
2	3.8994557908814803E-4	5.34172026148148E-6	0.0	5.34172026148148E-6	2.136688104592592E-5
3	4.273376209185184E-4	1.068344052296296E-5	0.0	1.068344052296296E-5	3.739204183037036E-5
4	4.487045019644443E-4	1.602516078444444E-5	0.0	1.068344052296296E-5	3.739204183037036E-5
5	4.593879424874073E-4	1.602516078444444E-5	0.0	1.068344052296296E-5	4.273376209185184E-5
6	4.8075482353333323E-4	1.602516078444444E-5	0.0	1.068344052296296E-5	4.8075482353333324E-5
7	4.967799843177776E-4	1.602516078444444E-5	0.0	1.068344052296296E-5	5.34172026148148E-5
8	5.074634248407406E-4	1.602516078444444E-5	0.0	1.068344052296296E-5	6.410064313777776E-5
9	5.234885856251851E-4	2.136688104592592E-5	0.0	2.136688104592592E-5	6.944236339925924E-5
10	5.395137464096295E-4	2.136688104592592E-5	0.0	2.136688104592592E-5	6.944236339925924E-5
11	5.608806274555554E-4	2.136688104592592E-5	0.0	2.67086013074074E-5	9.080924444518517E-5
12	5.822475085014813E-4	2.136688104592592E-5	0.0	2.67086013074074E-5	1.068344052296296E-4
13	5.982726692859258E-4	2.67086013074074E-5	0.0	2.67086013074074E-5	1.3888472679851848E-4
14	6.089561098088888E-4	2.67086013074074E-5	0.0	2.67086013074074E-5	1.5490988758296291E-4
15	6.356647111162961E-4	2.67086013074074E-5	0.0	2.67086013074074E-5	1.7627676862888884E-4
16	6.89081913731111E-4	2.67086013074074E-5	0.0	2.67086013074074E-5	1.869602091518518E-4
17	7.478408366074072E-4	2.67086013074074E-5	0.0	2.67086013074074E-5	2.0298536993629626E-4
18	7.852328784377776E-4	2.67086013074074E-5	0.0	3.205032156888888E-5	2.2969397124370365E-4
19	7.959163189607406E-4	2.67086013074074E-5	0.0	3.205032156888888E-5	2.6708601307407403E-4
20	8.653586823599998E-4	2.67086013074074E-5	0.0	3.205032156888888E-5	2.7776945359703697E-4
21	8.760421228829628E-4	2.67086013074074E-5	0.0	3.205032156888888E-5	2.884528941199999E-4
22	9.294593254977776E-4	2.67086013074074E-5	0.0	3.205032156888888E-5	3.258449359503703E-4
23	9.882182483740738E-4	2.67086013074074E-5	0.0	3.205032156888888E-5	3.3652837647333325E-4
24	0.0010576606117733331	2.67086013074074E-5	0.0	3.739204183037036E-5	3.4187009673481474E-4
25	0.0011110778143881479	2.67086013074074E-5	0.0	3.739204183037036E-5	3.525535372577777E-4
26	0.0011484698562185182	3.205032156888888E-5	0.0	3.739204183037036E-5	3.739204183037036E-4
27	0.0011965453385718516	3.205032156888888E-5	0.0	3.739204183037036E-5	3.846038588266666E-4
28	0.0012980380235399997	3.205032156888888E-5	0.0	3.739204183037036E-5	4.059707398725925E-4
29	0.0013728221072007404	3.205032156888888E-5	0.0	3.739204183037036E-5	4.1665418039555545E-4
30	0.0014743147921688884	3.205032156888888E-5	0.0	3.739204183037036E-5	4.273376209185184E-4
31	0.0015384154353066664	3.205032156888888E-5	0.0	4.273376209185184E-5	4.487045019644443E-4
32	0.0016505915607977774	3.205032156888888E-5	0.0	4.8075482353333324E-5	4.7541310327185173E-4
33	0.0017948180078577773	3.205032156888888E-5	0.0	5.34172026148148E-5	5.021217045792591E-4
34	0.001890968972564444	3.205032156888888E-5	0.0	5.34172026148148E-5	5.074634248407406E-4
35	0.0020405371398859256	3.739204183037036E-5	0.0	5.34172026148148E-5	5.181468653637036E-4
36	0.002158054985638518	3.739204183037036E-5	0.0	5.34172026148148E-5	5.288303058866666E-4
37	0.002270231111129629	3.739204183037036E-5	0.0	5.34172026148148E-5	5.44855466671111E-4
38	0.002403774117666666	3.739204183037036E-5	0.0	5.34172026148148E-5	5.44855466671111E-4
39	0.002531975403942222	4.273376209185184E-5	0.0	5.875892287629628E-5	5.822475085014813E-4
40	0.002654834969956296	4.273376209185184E-5	0.0	5.875892287629628E-5	5.982726692859258E-4
41	0.0027990614170162955	4.273376209185184E-5	0.0	5.875892287629628E-5	5.982726692859258E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	104465	0.0	43.267982	1
CAACGCA	113000	0.0	39.989513	13
TCAACGC	113025	0.0	39.98482	12
AGCAGTG	113695	0.0	39.79053	2
GTGGTAT	114005	0.0	39.770973	6
AACGCAG	113895	0.0	39.730976	14
AGTGGTA	114055	0.0	39.708206	5
GTATCAA	114025	0.0	39.702168	9
ACGCAGA	114005	0.0	39.665844	15
GGTATCA	114345	0.0	39.593113	8
ATCAACG	114250	0.0	39.586956	11
TGGTATC	114970	0.0	39.38401	7
TATCAAC	115000	0.0	39.33491	10
AGAGTAC	114035	0.0	39.24738	19
CGCAGAG	115790	0.0	39.074657	16
CAGTGGT	117145	0.0	38.644756	4
GCAGTGG	117650	0.0	38.488865	3
CAGAGTA	117265	0.0	38.298595	18
GCAGAGT	119225	0.0	37.816814	17
ACATGGG	85380	0.0	37.78771	24
>>END_MODULE