FastQCFastQC Report
Thu 26 May 2016
SRR805262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14784686
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4966883.3594761498485664No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC1143500.7734354317704143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG447710.3028200937104785No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG356550.24116169934214363No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA198070.13396970351619236No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG191660.12963413629481207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2882600.045.3385471
AGCAGTG2956650.044.2229042
GTGGTAT2958100.044.0900086
AGTGGTA2960500.044.0653765
TGGTATC2961350.044.023377
GTATCAA2965950.043.9923329
GGTATCA2963950.043.989518
ACGCAGA2970850.043.93717615
TATCAAC2968800.043.93426510
TCAACGC2974600.043.9094312
AACGCAG2974750.043.9080114
CAACGCA2977900.043.85998513
AGAGTAC2944450.043.82351319
ATCAACG2985450.043.77189311
CGCAGAG2981600.043.75984216
GCAGTGG2994100.043.6791883
CAGAGTA2968250.043.57188818
CAGTGGT2994350.043.5703744
GCAGAGT2990850.043.40685717
GAGTACT774150.043.3724720