##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805262_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14784686
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.468760648687436	37.0	35.0	37.0	32.0	37.0
2	35.884499407021565	37.0	35.0	37.0	35.0	37.0
3	37.844395680774014	39.0	38.0	39.0	35.0	39.0
4	37.88672150358824	39.0	39.0	39.0	35.0	39.0
5	37.877232090015305	39.0	38.0	39.0	35.0	39.0
6	37.915160321970994	39.0	39.0	39.0	35.0	39.0
7	37.85811318549477	39.0	38.0	39.0	35.0	39.0
8	38.8131531504964	40.0	39.0	40.0	36.0	40.0
9	38.864781639596536	40.0	40.0	40.0	36.0	40.0
10	38.55827252604485	40.0	39.0	40.0	36.0	40.0
11	38.704323784759445	40.0	39.0	40.0	36.0	40.0
12	38.77976637447694	40.0	39.0	40.0	36.0	40.0
13	38.89624825309107	40.0	40.0	40.0	37.0	40.0
14	38.88238052536253	40.0	40.0	40.0	37.0	40.0
15	38.869889627686376	40.0	39.0	40.0	37.0	40.0
16	38.8374213020148	40.0	39.0	40.0	36.0	40.0
17	38.84248924867258	40.0	39.0	40.0	36.0	40.0
18	38.77781780417927	40.0	39.0	40.0	36.0	40.0
19	38.747317596058515	40.0	39.0	40.0	36.0	40.0
20	38.711319739898435	40.0	39.0	40.0	36.0	40.0
21	38.61391510107148	40.0	39.0	40.0	36.0	40.0
22	38.58779219254301	40.0	39.0	40.0	36.0	40.0
23	38.17195650959378	40.0	38.0	40.0	35.0	40.0
24	38.325053234136995	40.0	38.0	40.0	35.0	40.0
25	38.42389145092429	40.0	39.0	40.0	35.0	40.0
26	38.39818491917921	40.0	39.0	40.0	35.0	40.0
27	38.42869256743092	40.0	39.0	40.0	35.0	40.0
28	38.33931542408138	40.0	39.0	40.0	35.0	40.0
29	38.26885697809206	40.0	39.0	40.0	35.0	40.0
30	38.25278967710238	40.0	38.0	40.0	35.0	40.0
31	38.140463043990245	40.0	38.0	40.0	34.0	40.0
32	38.0935812908032	40.0	38.0	40.0	34.0	40.0
33	37.968690373268664	40.0	38.0	40.0	34.0	40.0
34	37.96193047319368	40.0	38.0	40.0	34.0	40.0
35	37.95965575460987	40.0	38.0	40.0	34.0	40.0
36	37.74781277059249	40.0	37.0	40.0	33.0	40.0
37	37.619389617067284	40.0	37.0	40.0	33.0	40.0
38	37.596906691153265	40.0	37.0	40.0	33.0	40.0
39	37.46979401523982	40.0	37.0	40.0	33.0	40.0
40	37.344664066588905	40.0	37.0	40.0	33.0	40.0
41	37.280348395630455	40.0	36.0	40.0	33.0	40.0
42	37.22978851224842	40.0	36.0	40.0	33.0	40.0
43	37.14527275046626	40.0	36.0	40.0	33.0	40.0
44	37.030387388680424	40.0	35.0	40.0	32.0	40.0
45	36.98660025650866	40.0	35.0	40.0	32.0	40.0
46	36.927937055951006	39.0	35.0	40.0	32.0	40.0
47	36.95083933470078	40.0	35.0	40.0	32.0	40.0
48	36.98558833106094	40.0	35.0	40.0	32.0	40.0
49	36.948724646570106	39.0	35.0	40.0	32.0	40.0
50	36.79065290936852	39.0	35.0	40.0	32.0	40.0
51	36.57745507750384	39.0	35.0	40.0	31.0	40.0
52	36.299939545554096	39.0	35.0	40.0	31.0	40.0
53	35.90672943612059	38.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	8.0
11	70.0
12	123.0
13	296.0
14	635.0
15	1174.0
16	2158.0
17	3584.0
18	5385.0
19	7926.0
20	10902.0
21	15370.0
22	21188.0
23	27784.0
24	36990.0
25	48341.0
26	61966.0
27	77307.0
28	95781.0
29	116668.0
30	141898.0
31	171884.0
32	212025.0
33	269828.0
34	361033.0
35	522354.0
36	812484.0
37	1570280.0
38	2562924.0
39	7626315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.923363404538996	40.62214104513278	23.811564209074174	17.64293134125405
2	23.2081763522066	38.98061142455106	19.133615688557743	18.677596534684607
3	38.4210662302872	20.6759818909918	20.072526396570073	20.830425482150922
4	20.096050737905426	22.637667110414114	19.084713736903172	38.18156841477729
5	21.50991911495449	40.310900075929915	19.12570885847694	19.053471950638652
6	39.38129629536941	21.097999646390868	20.428015853701595	19.092688204538128
7	20.521355678436457	21.07291287755452	39.429474525194514	18.976256918814506
8	38.33919097098173	21.057288602544553	21.075834819894045	19.527685606579674
9	38.19979673562225	21.10334301316917	21.29851793944085	19.39834231176773
10	19.248450728003284	21.54034248681372	40.05416144786572	19.157045337317275
11	18.991820320025735	39.99108942861553	21.702111225087904	19.31497902627083
12	19.006869675825378	21.457587939304222	40.036663612605636	19.49887877226476
13	19.012537702863625	21.532685915683295	21.459109784272727	37.99566659718035
14	19.17743805989522	40.11816686536325	21.0860683818378	19.618326692903725
15	19.282195103771567	40.0291761353606	21.08249711897838	19.60613164188945
16	19.44203617175231	21.254208577713456	21.10182793195608	38.20192731857816
17	38.121695651838664	20.988041274599947	21.093684370435735	19.796578703125654
18	19.6064563021494	21.076308282773134	21.10616349917746	38.21107191590001
19	19.57863697612516	39.92007676050746	21.022205003203993	19.47908126016339
20	38.3152405130552	21.374731935463494	21.052026400831238	19.258001150650074
21	19.883540306503637	39.62049650564104	21.166550307527668	19.329412880327656
22	38.17195711833177	21.241688866439233	21.367508244679666	19.218845770549336
23	19.702711305468373	21.094658351215575	39.76553847677252	19.43709186654353
24	19.280795006400542	39.67712266598019	21.280587223834175	19.761495103785094
25	19.351753564465284	21.385628345437976	21.268459810374058	37.994158279722676
26	19.680154181157448	34.6475332651637	26.144329341860896	19.527983211817958
27	19.936074394816366	20.96678955508423	39.77858576096915	19.318550289130254
28	33.80283490633484	20.833367715756694	26.17436041590603	19.18943696200244
29	34.11416380435811	20.795524504206583	25.851066434552617	19.239245256882693
30	33.62915519477384	20.855309338324805	25.99112351794282	19.524411948958537
31	28.25270688873609	22.94085921067245	27.27871934513861	21.52771455545285
32	24.752355241092033	25.507318856822526	28.184406486549662	21.55591941553578
33	24.31766220804419	24.651920236926237	28.701231801608774	22.329185753420802
34	23.55200509500168	23.723222799591415	29.821323225937974	22.903448879468932
35	22.86760097576641	24.512600402876327	29.57544718907118	23.044351432286085
36	22.97684238948328	24.701687949274	29.56739832012665	22.754071341116074
37	23.909280183562913	23.910605879624363	29.846809056343844	22.333304880468884
38	23.97779026216722	23.974158125509057	29.788600177237445	22.259451435086277
39	24.43448579158191	23.979717932460655	29.208614914107745	22.377181361849686
40	23.326920842282345	24.44858145786796	28.946228550271545	23.27826914957815
41	23.469446696399235	25.514677822714667	28.712202612892828	22.303672867993274
42	23.660340165492862	25.32469746060214	28.901533654485455	22.113428719419538
43	23.574792186996735	24.26884818521002	30.10562415732062	22.050735470472624
44	23.485659418130357	23.909070507145028	29.325181474939676	23.28008859978494
45	23.66475689777923	23.654597737145046	29.081963593951198	23.59868177112453
46	23.52764881175021	23.781343749877408	28.80863347385261	23.882373964519772
47	23.332649743119333	23.88775115007515	28.897948864115207	23.88165024269031
48	23.733321086426862	23.928009022308625	29.105014472407465	23.23365541885705
49	22.671763201464003	25.366017242435856	28.671620080399407	23.290599475700734
50	23.5212570628825	24.579263976252182	29.111974376730082	22.78750458413523
51	23.358710492735526	24.198809497881797	28.87332203064712	23.569157978735564
52	23.73243503446742	24.05706823939311	28.55493853572541	23.655558190414055
53	24.03166357405223	24.30023877409368	27.95996479059481	23.70813286125928
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1490.0
1	983.5
2	477.0
3	465.0
4	453.0
5	462.5
6	472.0
7	593.5
8	852.5
9	990.0
10	1419.0
11	1848.0
12	2668.0
13	3488.0
14	4770.0
15	6052.0
16	8919.5
17	11787.0
18	16570.5
19	21354.0
20	30196.5
21	39039.0
22	328649.5
23	618260.0
24	394649.5
25	165837.5
26	160636.0
27	169519.5
28	178403.0
29	194299.0
30	210195.0
31	227665.5
32	245136.0
33	273854.0
34	302572.0
35	340911.5
36	379251.0
37	423003.0
38	466755.0
39	514077.0
40	561399.0
41	617222.0
42	732432.0
43	791819.0
44	863555.5
45	935292.0
46	994978.5
47	1054665.0
48	1144587.5
49	1234510.0
50	1205719.0
51	1176928.0
52	1152826.0
53	1128724.0
54	1102924.0
55	1077124.0
56	1040411.0
57	1003698.0
58	874718.0
59	638247.0
60	530756.0
61	460893.5
62	391031.0
63	337318.5
64	283606.0
65	233610.5
66	183615.0
67	143377.0
68	103139.0
69	80789.5
70	58440.0
71	39154.0
72	19868.0
73	12916.5
74	5965.0
75	4231.0
76	1831.5
77	1166.0
78	872.0
79	578.0
80	449.0
81	320.0
82	238.0
83	156.0
84	123.5
85	91.0
86	69.0
87	47.0
88	39.5
89	32.0
90	20.5
91	9.0
92	7.5
93	4.5
94	3.0
95	3.0
96	3.0
97	2.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.4784686E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.78003015480619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64586320951649	32.67998142244169
2	12.89206821203743	11.28830270161634
3	4.119102645989751	5.410033141565198
4	2.024312047659226	3.544977699570335
5	1.181497038842801	2.586298799417602
6	0.798447535303743	2.0973634303577127
7	0.6152471848335005	1.8854878213269186
8	0.4858990565466595	1.7018140278243703
9	0.4087319086522578	1.6104865757424598
>10	2.6870456321796117	20.70093294606612
>50	0.08959150186082279	2.6399332073307282
>100	0.04143200974803223	3.4707437577403604
>500	0.007424184179980002	2.299336507254028
>1k	0.0030137858863254863	2.0477137305775037
>5k	1.6202338167070927E-4	0.5006017091036552
>10k+	1.6202338167070927E-4	5.535992522064998
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	496688	3.3594761498485664	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	114350	0.7734354317704143	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	44771	0.3028200937104785	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	35655	0.24116169934214363	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	19807	0.13396970351619236	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	19166	0.12963413629481207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2851135289582749E-4	0.0	0.0	0.0	6.763755415569868E-6
2	1.2851135289582749E-4	0.0	0.0	0.0	1.3527510831139736E-5
3	1.2851135289582749E-4	0.0	0.0	0.0	2.705502166227947E-5
4	1.5556637455810694E-4	0.0	0.0	0.0	5.411004332455894E-5
5	1.5556637455810694E-4	0.0	0.0	0.0	7.440130957126854E-5
6	1.623301299736768E-4	0.0	0.0	0.0	8.11650649868384E-5
7	1.893851516359563E-4	0.0	0.0	0.0	8.11650649868384E-5
8	1.893851516359563E-4	0.0	0.0	0.0	8.11650649868384E-5
9	2.0291266246709602E-4	0.0	0.0	0.0	9.469257581797815E-5
10	2.3673143954494537E-4	0.0	0.0	0.0	1.0145633123354801E-4
11	2.7055021662279467E-4	0.0	0.0	0.0	1.0145633123354801E-4
12	2.9084148286950427E-4	0.0	0.0	6.763755415569868E-6	1.0145633123354801E-4
13	3.246602599473536E-4	0.0	0.0	6.763755415569868E-6	1.1498384206468775E-4
14	3.58479037025203E-4	0.0	0.0	6.763755415569868E-6	1.2174759748025761E-4
15	3.787703032719126E-4	0.0	0.0	1.3527510831139736E-5	1.3527510831139734E-4
16	4.0582532493419203E-4	0.0	0.0	1.3527510831139736E-5	1.623301299736768E-4
17	4.193528357653318E-4	0.0	0.0	1.3527510831139736E-5	1.8262139622038641E-4
18	4.7346287908989074E-4	0.0	0.0	1.3527510831139736E-5	1.893851516359563E-4
19	4.8699038992103044E-4	0.0	0.0	1.3527510831139736E-5	2.0291266246709602E-4
20	5.140454115833099E-4	0.0	0.0	2.02912662467096E-5	2.0291266246709602E-4
21	5.411004332455893E-4	0.0	0.0	3.3818777077849334E-5	2.1644017329823577E-4
22	5.61391699492299E-4	0.0	0.0	3.3818777077849334E-5	2.3673143954494537E-4
23	5.749192103234387E-4	0.0	0.0	3.3818777077849334E-5	2.5025895037608507E-4
24	6.08737987401288E-4	0.0	0.0	3.3818777077849334E-5	2.637864612072248E-4
25	6.560842753102771E-4	0.0	0.0	3.3818777077849334E-5	2.7055021662279467E-4
26	6.899030523881265E-4	0.0	0.0	4.05825324934192E-5	2.7055021662279467E-4
27	7.304855848815457E-4	0.0	0.0	4.05825324934192E-5	3.246602599473536E-4
28	7.845956282061047E-4	0.0	0.0	4.05825324934192E-5	3.381877707784934E-4
29	8.454694269462334E-4	0.0	0.0	4.05825324934192E-5	3.381877707784934E-4
30	8.725244486085129E-4	0.0	0.0	4.05825324934192E-5	3.4495152619406323E-4
31	9.333982473486417E-4	0.0	0.0	4.05825324934192E-5	3.517152816096331E-4
32	0.0010010358015043403	0.0	0.0	4.05825324934192E-5	3.58479037025203E-4
33	0.0010889646219067487	0.0	0.0	4.734628790898907E-5	3.58479037025203E-4
34	0.0011092558881534583	0.0	0.0	4.734628790898907E-5	3.58479037025203E-4
35	0.0011363109098157377	0.0	0.0	4.734628790898907E-5	3.787703032719126E-4
36	0.0012445309964648555	0.0	0.0	4.734628790898907E-5	3.9229781410305233E-4
37	0.0013121685506205542	0.0	0.0	4.734628790898907E-5	3.990615695186222E-4
38	0.001373042349360683	0.0	0.0	5.411004332455894E-5	4.0582532493419203E-4
39	0.0014947899468409408	0.0	0.0	5.411004332455894E-5	4.0582532493419203E-4
40	0.0016165375443211983	0.0	0.0	5.411004332455894E-5	4.1258908034976194E-4
41	0.001738285141801456	0.0	0.0	6.0873798740128805E-5	4.1258908034976194E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	288260	0.0	45.338547	1
AGCAGTG	295665	0.0	44.222904	2
GTGGTAT	295810	0.0	44.090008	6
AGTGGTA	296050	0.0	44.065376	5
TGGTATC	296135	0.0	44.02337	7
GTATCAA	296595	0.0	43.992332	9
GGTATCA	296395	0.0	43.98951	8
ACGCAGA	297085	0.0	43.937176	15
TATCAAC	296880	0.0	43.934265	10
TCAACGC	297460	0.0	43.90943	12
AACGCAG	297475	0.0	43.90801	14
CAACGCA	297790	0.0	43.859985	13
AGAGTAC	294445	0.0	43.823513	19
ATCAACG	298545	0.0	43.771893	11
CGCAGAG	298160	0.0	43.759842	16
GCAGTGG	299410	0.0	43.679188	3
CAGAGTA	296825	0.0	43.571888	18
CAGTGGT	299435	0.0	43.570374	4
GCAGAGT	299085	0.0	43.406857	17
GAGTACT	77415	0.0	43.37247	20
>>END_MODULE