FastQCFastQC Report
Thu 26 May 2016
SRR805261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18399960
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10269885.581468655366642No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC1486490.8078767562538179No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG676920.36789210411326984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG480900.2613592638244866No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA428890.2330928980280392No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG378280.20558740345087706No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC287220.1560981654307944No Hit
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT213550.11606003491311936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5403350.045.2351071
AGCAGTG5463050.044.808752
GTGGTAT5459650.044.72136
AGTGGTA5464750.044.698915
TGGTATC5462750.044.6825837
GTATCAA5473900.044.6636929
AGAGTAC5404500.044.63847419
GGTATCA5475000.044.6366928
TATCAAC5480250.044.59650810
ACGCAGA5482050.044.5857215
AACGCAG5488850.044.55745714
GAGTACT1553950.044.55162420
TCAACGC5487350.044.54308312
CAACGCA5490100.044.5344713
CGCAGAG5493900.044.47115716
GTACTTT1541000.044.4624222
ATCAACG5504100.044.45577611
GCAGTGG5506650.044.4351923
CAGTGGT5496900.044.4327774
CAGAGTA5454300.044.4006618
Produced by FastQC (version 0.11.5)