##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805261_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18399960
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.50562811006111	35.0	35.0	35.0	32.0	37.0
2	35.611149698151515	37.0	35.0	37.0	35.0	37.0
3	37.912762092961074	39.0	38.0	39.0	35.0	39.0
4	37.942888191061286	39.0	38.0	39.0	35.0	39.0
5	37.89719580912132	39.0	38.0	39.0	35.0	39.0
6	37.953761856004036	39.0	38.0	39.0	35.0	39.0
7	37.81354421422655	39.0	38.0	39.0	35.0	39.0
8	38.81738297257168	40.0	39.0	40.0	36.0	40.0
9	38.893322485483665	40.0	39.0	40.0	36.0	40.0
10	38.44933711812417	40.0	38.0	40.0	36.0	40.0
11	38.70542060961002	40.0	39.0	40.0	36.0	40.0
12	38.8394298683258	40.0	39.0	40.0	36.0	40.0
13	38.95911045458794	40.0	40.0	40.0	37.0	40.0
14	38.98386447579234	40.0	40.0	40.0	37.0	40.0
15	38.96590128456801	40.0	40.0	40.0	37.0	40.0
16	38.892098026300054	40.0	39.0	40.0	37.0	40.0
17	38.89585912143287	40.0	39.0	40.0	37.0	40.0
18	38.849844021400045	40.0	40.0	40.0	36.0	40.0
19	38.81753732073331	40.0	39.0	40.0	36.0	40.0
20	38.7740102152396	40.0	39.0	40.0	36.0	40.0
21	38.67043971834721	40.0	39.0	40.0	36.0	40.0
22	38.661625840490956	40.0	39.0	40.0	36.0	40.0
23	38.012229428759625	40.0	38.0	40.0	35.0	40.0
24	38.257718386344315	40.0	38.0	40.0	35.0	40.0
25	38.39639488346714	40.0	38.0	40.0	35.0	40.0
26	38.422810538718565	40.0	39.0	40.0	35.0	40.0
27	38.47218483083659	40.0	39.0	40.0	35.0	40.0
28	38.412445733577684	40.0	39.0	40.0	35.0	40.0
29	38.3642658462301	40.0	39.0	40.0	35.0	40.0
30	38.336204752619025	40.0	39.0	40.0	35.0	40.0
31	38.16811726764623	40.0	38.0	40.0	35.0	40.0
32	38.084182465614056	40.0	38.0	40.0	34.0	40.0
33	37.93080767566886	40.0	38.0	40.0	34.0	40.0
34	37.96555987078233	40.0	38.0	40.0	34.0	40.0
35	37.92783098441518	40.0	38.0	40.0	34.0	40.0
36	37.778394409553066	40.0	38.0	40.0	33.0	40.0
37	37.46400198696084	40.0	36.0	40.0	33.0	40.0
38	37.40078184952576	40.0	36.0	40.0	33.0	40.0
39	37.29738493996726	40.0	36.0	40.0	33.0	40.0
40	37.18427072667549	40.0	35.0	40.0	33.0	40.0
41	37.11311160459044	40.0	35.0	40.0	33.0	40.0
42	37.05031021806569	40.0	35.0	40.0	33.0	40.0
43	36.95500153261203	39.0	35.0	40.0	32.0	40.0
44	36.86779906043274	39.0	35.0	40.0	32.0	40.0
45	36.819840260522305	39.0	35.0	40.0	32.0	40.0
46	36.74589173019941	39.0	35.0	40.0	32.0	40.0
47	36.818257920125916	39.0	35.0	40.0	32.0	40.0
48	36.842175037337036	39.0	35.0	40.0	32.0	40.0
49	36.77710897197603	39.0	35.0	40.0	32.0	40.0
50	36.57746348361627	39.0	35.0	40.0	32.0	40.0
51	36.30228158104691	39.0	35.0	40.0	31.0	40.0
52	35.973808747410324	39.0	35.0	40.0	30.0	40.0
53	35.495305641968784	38.0	35.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	10.0
13	61.0
14	164.0
15	429.0
16	977.0
17	1971.0
18	3295.0
19	5646.0
20	8670.0
21	13241.0
22	19009.0
23	27308.0
24	38184.0
25	53085.0
26	69885.0
27	90593.0
28	113355.0
29	139606.0
30	169472.0
31	210342.0
32	266048.0
33	345423.0
34	482864.0
35	722054.0
36	1175668.0
37	2335899.0
38	3643939.0
39	8462756.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.327225711360242	49.78398866084491	30.232761375568206	8.656024252226635
2	22.702016743514662	54.78337452907507	9.884668227539626	12.629940499870651
3	50.805094141509	20.677506907623712	14.812244157052515	13.705154793814769
4	19.98653257941865	18.647698147169887	14.879412781332132	46.48635649207933
5	16.92097700212392	48.24695814556119	17.680011260894048	17.152053591420852
6	46.15375794295205	19.519656564470793	16.168796019121782	18.157789473455377
7	18.631149198150432	18.16591449111846	46.33752464679271	16.865411663938403
8	45.05314685466708	19.834635510077195	18.340485522794616	16.771732112461113
9	46.00346957275994	19.192079765390794	17.963794486509755	16.84065617533951
10	17.564538183778662	19.53020550044674	46.4072802332179	16.4979760825567
11	16.7306885449751	47.592130635066596	19.062286004969575	16.61489481498873
12	16.7021558742519	19.784928880280177	46.88734649423151	16.625568751236415
13	16.55039467477103	19.462618396996515	19.255438598779563	44.73154832945289
14	16.649416629166584	47.700342826832234	18.584790401718266	17.065450142282916
15	16.77232450505327	47.87715299381085	18.658964476009732	16.69155802512614
16	16.702199352607288	19.41345524664184	18.881323655051425	45.00302174569944
17	44.95545642490527	19.0573349072498	18.83976378209518	17.147444885749753
18	17.275885382359526	18.918551996852166	18.84395400859567	44.961608612192634
19	16.754655988382584	47.49975543425094	18.753187506929365	16.99240107043711
20	45.38137582907789	19.55571099067607	18.64875793208246	16.414155248163585
21	17.420510696762385	47.150227500494566	18.77023645703578	16.659025345707274
22	45.1340274652771	19.514863075789297	18.82720397218255	16.523905486751058
23	17.410651979678214	19.028307670234064	46.76936254209249	16.791677807995235
24	16.922569396889994	47.06164578618649	19.028291365850794	16.98749345107272
25	16.789237585299098	19.629972021678306	18.77111689373238	44.80967349929021
26	17.189542803353923	38.974437987908665	26.64406335665947	17.19195585207794
27	17.38787475624947	19.185623229615718	46.66075361033394	16.765748403800877
28	37.38810845240968	18.901883482355398	27.14406987841278	16.565938186822144
29	37.91608786106057	18.95175315598512	26.57162298178909	16.560536001165218
30	37.45524446792275	18.83193767812539	26.799531085937144	16.913286768014714
31	29.511069589281714	21.97560212087418	28.724024400053043	19.789303889791064
32	24.16381883438877	25.591805634360078	30.126978536909867	20.11739699434129
33	23.508822845267055	24.18440583566486	31.063736008121758	21.24303531094633
34	22.48754888597584	23.18096887167146	32.34455944469444	21.986922797658256
35	21.692242809223497	23.925872664940577	32.288657149254675	22.093227376581254
36	21.84189530846806	24.162161222089612	32.26484731488547	21.73109615455686
37	22.909941108567626	23.352773592986072	32.496842384439965	21.240442914006337
38	23.11688721062437	23.430360718175475	32.2757386429101	21.177013428290064
39	23.57161102524136	23.327469190150413	31.784237574429508	21.316682210178715
40	22.23321137654647	24.010927197667822	31.475573859943175	22.280287565842535
41	22.589494759771217	25.20484283661486	30.984898880214956	21.220763523398965
42	22.93117485038011	25.019434824858315	31.11572525157663	20.933665073184944
43	22.846707275450598	23.852894245422274	32.60234804858271	20.698050430544416
44	22.76448427061798	23.652312287635407	31.46032926158535	22.12287418016126
45	22.82323439833565	23.102821962656442	31.395106293709336	22.67883734529858
46	22.57590777371255	23.24864293183246	31.204415661773176	22.971033632681813
47	22.63425029184846	23.500768479931477	31.10926871580156	22.755712512418505
48	22.980321696351513	23.418290039760954	31.324796358252954	22.276591905634575
49	21.685242794006072	24.95952708592845	31.0121217654821	22.34310835458338
50	22.822006134795945	24.17019928304192	31.199089563238182	21.808705018923956
51	22.51628807888713	23.853111637199213	30.92958354257292	22.701016741340744
52	23.04858814910467	23.70616023078311	30.57894147595973	22.666310144152487
53	23.573909943282487	24.06579144737271	29.621314394161725	22.738984215183073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1638.0
1	1101.0
2	564.0
3	561.5
4	559.0
5	565.5
6	572.0
7	662.0
8	958.0
9	1164.0
10	1723.5
11	2283.0
12	3323.5
13	4364.0
14	5932.0
15	7500.0
16	11059.0
17	14618.0
18	20614.0
19	26610.0
20	39928.5
21	53247.0
22	644354.0
23	1235461.0
24	758848.5
25	258760.0
26	235284.0
27	239306.5
28	243329.0
29	257505.0
30	271681.0
31	290618.0
32	309555.0
33	345988.0
34	382421.0
35	431051.5
36	479682.0
37	534019.0
38	588356.0
39	648839.0
40	709322.0
41	777213.5
42	916961.0
43	988817.0
44	1082717.0
45	1176617.0
46	1241389.0
47	1306161.0
48	1426480.5
49	1546800.0
50	1490166.0
51	1433532.0
52	1395251.0
53	1356970.0
54	1317694.5
55	1278419.0
56	1232374.0
57	1186329.0
58	1014897.0
59	717445.0
60	591425.0
61	496378.0
62	401331.0
63	336173.0
64	271015.0
65	217625.0
66	164235.0
67	126084.0
68	87933.0
69	66007.5
70	44082.0
71	30017.0
72	15952.0
73	11146.0
74	6340.0
75	4459.0
76	1719.0
77	860.0
78	599.5
79	339.0
80	263.5
81	188.0
82	152.0
83	116.0
84	88.5
85	61.0
86	47.5
87	34.0
88	27.0
89	20.0
90	18.0
91	16.0
92	11.5
93	7.0
94	7.0
95	5.5
96	4.0
97	3.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.839996E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.19802553306438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.993439316213	27.8820525874689
2	13.821555746539998	10.559122786260152
3	4.444357409071653	5.092970333693487
4	2.1728748470100765	3.3199811554497707
5	1.2835454501817984	2.4514450939446477
6	0.8362133922577502	1.916502030513115
7	0.5628254905236122	1.5049175720376298
8	0.4491171565844221	1.3724310891639222
9	0.3453826741139503	1.1873642584034454
>10	2.850153816567829	22.78126408239088
>50	0.16527820833485274	4.258702500241976
>100	0.06603604824114104	4.679864294189382
>500	0.005554960444184754	1.455079864479052
>1k	0.0032256941126625758	2.3717322831452803
>5k	2.0523524144577218E-4	0.4897610234374362
>10k+	2.3455456165231102E-4	8.676809045180903
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1026988	5.581468655366642	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	148649	0.8078767562538179	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	67692	0.36789210411326984	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	48090	0.2613592638244866	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	42889	0.2330928980280392	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	37828	0.20558740345087706	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	28722	0.1560981654307944	No Hit
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21355	0.11606003491311936	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7717451559677305E-4	0.0	0.0	0.0	5.434794423466138E-6
2	0.0024945706403709574	0.0	0.0	0.0	5.434794423466138E-6
3	0.002516309818064822	0.0	0.0	0.0	1.0869588846932276E-5
4	0.0028152235113554597	0.0	0.0	0.0	1.0869588846932276E-5
5	0.002847832277896256	0.0	0.0	0.0	1.6304383270398415E-5
6	0.0028641366611666546	0.0	0.0	0.0	2.1739177693864553E-5
7	0.002918484605401316	0.0	0.0	0.0	2.1739177693864553E-5
8	0.0029565281663655792	0.0	0.0	0.0	2.1739177693864553E-5
9	0.0029837021384829096	0.0	0.0	0.0	3.260876654079683E-5
10	0.0030163109050237065	0.0	0.0	0.0	3.8043560964262964E-5
11	0.003038050082717571	0.0	0.0	0.0	5.978273865812752E-5
12	0.003070658849258368	0.0	0.0	5.434794423466138E-6	7.065232750505979E-5
13	0.003309789803890878	0.0	0.0	1.0869588846932276E-5	8.152191635199207E-5
14	0.0034130508979367345	0.0	0.0	1.0869588846932276E-5	9.239150519892435E-5
15	0.0036467470581457787	0.0	0.0	1.0869588846932276E-5	1.0326109404585662E-4
16	0.0036793558246865756	0.0	0.0	1.0869588846932276E-5	1.1956547731625504E-4
17	0.003690225413533508	0.0	0.0	1.0869588846932276E-5	1.2500027173972117E-4
18	0.0038260952741201613	0.0	0.0	1.0869588846932276E-5	1.3586986058665345E-4
19	0.0038478344518140255	0.0	0.0	1.0869588846932276E-5	1.4130465501011958E-4
20	0.0038804432183548224	0.0	0.0	1.0869588846932276E-5	1.57609038280518E-4
21	0.003961965134706815	0.0	0.0	1.0869588846932276E-5	1.57609038280518E-4
22	0.004021747873364942	0.0	0.0	1.0869588846932276E-5	1.6304383270398414E-4
23	0.004201096089339325	0.0	0.0	2.1739177693864553E-5	1.6304383270398414E-4
24	0.004271748416844384	0.0	0.0	2.717397211733069E-5	1.6847862712745027E-4
25	0.0043152267722321135	0.0	0.0	2.717397211733069E-5	1.6847862712745027E-4
26	0.00439131389416064	0.0	0.0	2.717397211733069E-5	1.7391342155091642E-4
27	0.004635879643216615	0.0	0.0	2.717397211733069E-5	1.7934821597438255E-4
28	0.004711966765145141	0.0	0.0	2.717397211733069E-5	1.9565259924478096E-4
29	0.005228272235374425	5.434794423466138E-6	0.0	2.717397211733069E-5	1.9565259924478096E-4
30	0.005331533329420281	5.434794423466138E-6	0.0	2.717397211733069E-5	2.0652218809171324E-4
31	0.005625012228287453	5.434794423466138E-6	0.0	2.717397211733069E-5	2.0652218809171324E-4
32	0.005673925378098648	1.0869588846932276E-5	0.0	3.260876654079683E-5	2.1739177693864552E-4
33	0.005711968939062911	1.6304383270398415E-5	0.0	3.260876654079683E-5	2.282613657855778E-4
34	0.005744577705603708	1.6304383270398415E-5	0.0	3.260876654079683E-5	2.282613657855778E-4
35	0.005793490855414903	2.1739177693864553E-5	0.0	3.8043560964262964E-5	2.3369616020904393E-4
36	0.005869577977343429	2.1739177693864553E-5	0.0	3.8043560964262964E-5	2.3369616020904393E-4
37	0.005972839071389286	2.1739177693864553E-5	0.0	3.8043560964262964E-5	2.3369616020904393E-4
38	0.00609240454870554	2.717397211733069E-5	0.0	3.8043560964262964E-5	2.3913095463251008E-4
39	0.006157622081787134	2.717397211733069E-5	0.0	4.3478355387729105E-5	2.3913095463251008E-4
40	0.006255448381409525	2.717397211733069E-5	0.0	4.3478355387729105E-5	2.5000054347944234E-4
41	0.006358709475455381	2.717397211733069E-5	0.0	4.3478355387729105E-5	2.8260931002023915E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	540335	0.0	45.235107	1
AGCAGTG	546305	0.0	44.80875	2
GTGGTAT	545965	0.0	44.7213	6
AGTGGTA	546475	0.0	44.69891	5
TGGTATC	546275	0.0	44.682583	7
GTATCAA	547390	0.0	44.663692	9
AGAGTAC	540450	0.0	44.638474	19
GGTATCA	547500	0.0	44.636692	8
TATCAAC	548025	0.0	44.596508	10
ACGCAGA	548205	0.0	44.58572	15
AACGCAG	548885	0.0	44.557457	14
GAGTACT	155395	0.0	44.551624	20
TCAACGC	548735	0.0	44.543083	12
CAACGCA	549010	0.0	44.53447	13
CGCAGAG	549390	0.0	44.471157	16
GTACTTT	154100	0.0	44.46242	22
ATCAACG	550410	0.0	44.455776	11
GCAGTGG	550665	0.0	44.435192	3
CAGTGGT	549690	0.0	44.432777	4
CAGAGTA	545430	0.0	44.40066	18
>>END_MODULE