FastQCFastQC Report
Thu 26 May 2016
SRR805260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38589221
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10752922.7865086988928853No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC1597200.41389796388996813No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG716620.18570470753996304No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA535280.13871231036252327No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG493010.12775847431592363No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG468920.1215157984142774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6620450.045.0324551
GTGGTAT6714750.044.3986326
AGAGTAC6623350.044.34570319
TCAACGC6759850.044.32177712
AGTGGTA6716650.044.3167955
GTATCAA6732200.044.3121729
GGTATCA6728550.044.312118
CAACGCA6777300.044.2596713
AACGCAG6806750.044.24045614
TGGTATC6718100.044.2295767
ACGCAGA6744950.044.22422815
ATCAACG6780850.044.20842711
TATCAAC6761550.044.18620710
AGCAGTG6758800.044.132562
CGCAGAG6764550.044.0575316
GCAGTGG6785550.043.906293
CAGAGTA6735900.043.88697418
CAGTGGT6794900.043.8071374
GTACATG4634150.043.7864822
GAGTACA4689250.043.678420
Produced by FastQC (version 0.11.5)