##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805260_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38589221
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.91132829553621	37.0	35.0	37.0	35.0	37.0
2	36.25268019792367	37.0	36.0	37.0	35.0	37.0
3	37.86613694534025	39.0	39.0	39.0	35.0	39.0
4	37.95577969817012	39.0	39.0	39.0	35.0	39.0
5	37.85311631452731	39.0	38.0	39.0	35.0	39.0
6	37.923143226964854	39.0	39.0	39.0	35.0	39.0
7	37.780094498409284	39.0	38.0	39.0	35.0	39.0
8	38.76574240770499	40.0	39.0	40.0	36.0	40.0
9	38.77087679484382	40.0	39.0	40.0	36.0	40.0
10	38.49579648161335	40.0	38.0	40.0	36.0	40.0
11	38.66309182556445	40.0	39.0	40.0	36.0	40.0
12	38.64375155435244	40.0	39.0	40.0	36.0	40.0
13	38.63273422907397	40.0	39.0	40.0	36.0	40.0
14	38.74170520311877	40.0	39.0	40.0	36.0	40.0
15	38.6503707343561	40.0	39.0	40.0	36.0	40.0
16	38.671871297946126	40.0	39.0	40.0	36.0	40.0
17	38.627882045092335	40.0	39.0	40.0	36.0	40.0
18	38.50976838843158	40.0	39.0	40.0	36.0	40.0
19	38.465850476743235	40.0	38.0	40.0	36.0	40.0
20	38.38781669109102	40.0	38.0	40.0	36.0	40.0
21	37.97704623267725	40.0	38.0	40.0	34.0	40.0
22	38.14242259515941	40.0	38.0	40.0	35.0	40.0
23	37.942551936977424	40.0	38.0	40.0	34.0	40.0
24	37.93582591366641	40.0	38.0	40.0	34.0	40.0
25	37.8271586772897	40.0	38.0	40.0	34.0	40.0
26	37.88554264933205	40.0	38.0	40.0	34.0	40.0
27	37.51648282819703	40.0	38.0	40.0	34.0	40.0
28	37.58010743466421	40.0	37.0	40.0	34.0	40.0
29	37.445090171682914	39.0	37.0	40.0	33.0	40.0
30	37.35642310582015	39.0	37.0	40.0	33.0	40.0
31	37.13656541550813	39.0	37.0	40.0	33.0	40.0
32	36.75191186160509	38.0	36.0	40.0	32.0	40.0
33	36.68605582890621	38.0	36.0	40.0	31.0	40.0
34	36.61809381951504	38.0	36.0	40.0	31.0	40.0
35	36.38397880589505	38.0	36.0	40.0	31.0	40.0
36	36.25209368699099	38.0	35.0	40.0	31.0	40.0
37	35.889880466879596	38.0	35.0	39.0	30.0	40.0
38	35.80130091250093	38.0	35.0	39.0	30.0	40.0
39	35.62671104451681	38.0	35.0	39.0	30.0	40.0
40	35.46457273651624	37.0	34.0	39.0	30.0	40.0
41	35.30818137012924	37.0	34.0	38.0	30.0	40.0
42	35.052878082198134	37.0	34.0	38.0	30.0	40.0
43	34.85528000163569	36.0	34.0	38.0	29.0	40.0
44	34.84264655148131	36.0	34.0	38.0	29.0	40.0
45	34.57499276805821	36.0	33.0	38.0	28.0	39.0
46	33.63683065797053	36.0	33.0	38.0	25.0	39.0
47	33.49665210914727	35.0	32.0	38.0	25.0	39.0
48	33.68852278723118	35.0	32.0	38.0	26.0	39.0
49	33.656926606525694	35.0	32.0	38.0	27.0	39.0
50	33.50457232603892	35.0	32.0	38.0	27.0	39.0
51	32.93131325454846	35.0	31.0	38.0	25.0	39.0
52	32.51452621964045	34.0	31.0	37.0	24.0	38.0
53	34.617843412801726	37.0	33.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	17.0
12	74.0
13	232.0
14	720.0
15	1850.0
16	3732.0
17	6394.0
18	10341.0
19	17137.0
20	29315.0
21	46443.0
22	69197.0
23	95386.0
24	127805.0
25	166290.0
26	210806.0
27	263845.0
28	326430.0
29	400182.0
30	490217.0
31	608862.0
32	773295.0
33	1023861.0
34	1456838.0
35	2272732.0
36	4205065.0
37	9363886.0
38	1.5569366E7
39	1048898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.446090088214012	41.50734734966534	28.826661206765486	18.219901355355166
2	25.08712730946292	47.2248299596408	12.160812989720625	15.527229741175653
3	40.19722502301873	24.034126006327	17.391470535256463	18.377178435397802
4	20.716380877447616	22.07129809642957	18.517842067866567	38.69447895825625
5	19.158090804683514	39.03358401559856	21.69864221928709	20.109682960430842
6	36.62449159054027	22.14234902539235	20.21196022588795	21.021199158179428
7	21.047579581873393	21.358016011776968	37.66746936923137	19.92693503711827
8	36.08227541053498	23.092443353546837	21.47848488571459	19.346796350203597
9	36.28894711297748	22.482881942602575	21.694716252499628	19.533454691920316
10	19.81772319270192	22.768554980677116	38.01354010229955	19.40018172432141
11	19.36578092623326	39.146968009538206	22.02904018197206	19.45821088225647
12	19.289806342553533	22.71753036942622	38.365765403763916	19.62689788425633
13	19.42235631032821	22.71593666013626	22.087328479629065	35.77437854990646
14	19.5980297192317	39.27730233269026	21.714416054161862	19.41025189391618
15	19.400573025301547	39.114645512020054	21.796252378351976	19.688529084326422
16	19.623935917234505	22.36645823972451	21.970008153313074	36.03959768972791
17	36.12688113087331	22.232656627092833	21.93108277567977	19.709379466354086
18	19.606710899916845	22.3983194685376	21.94342560063599	36.05154403090957
19	19.659920059023737	38.87881281666712	21.91132596327871	19.549941161030436
20	36.3035548191035	22.53704991868066	21.91072216772658	19.24867309448926
21	19.960047910788354	38.58278973809811	21.90982554428865	19.54733680682489
22	35.93701723079613	22.299063772238366	22.269286026789707	19.49463297017579
23	19.77223639730898	22.357528284906294	38.3741459823716	19.49608933541312
24	19.64796594365043	38.52656937542222	22.03202806296608	19.79343661796127
25	19.642469590147986	22.45935983004166	22.01579555078347	35.88237502902689
26	19.838723875768313	33.582341555949	26.94872746977712	19.630207098505565
27	20.36415298458603	22.240612216556535	37.98944269955592	19.40579209930151
28	31.23559555659338	22.123499202018095	27.089479209751342	19.551426031637178
29	31.405334665864338	22.220777662238895	26.84915562301711	19.52473204887966
30	31.161688907894774	22.184713187135856	27.00933506794553	19.64426283702384
31	26.695112606704342	23.884221451373687	28.183339072846277	21.237326869075694
32	24.097112507142864	25.915304172634112	28.530995741012756	21.456587579210268
33	23.36968916786374	25.106176670423068	29.415652106581785	22.108482055131407
34	22.729202022502605	24.497364173275226	30.180738813048336	22.592694991173833
35	22.653686116130718	25.033438741870427	29.886262798619335	22.426612343379514
36	22.458204066881784	25.199809034756104	29.979965130677293	22.362021767684816
37	23.08704547313873	24.452058775687647	30.07058629144133	22.39030945973229
38	23.264413655823734	24.77114528950973	29.926787068337035	22.0376539863295
39	23.572201677769034	24.67923102153319	29.657017434998235	22.091549865699545
40	22.66242949034913	24.987182301503314	29.55218764327997	22.79820056486758
41	22.973609651254687	25.75747253358652	29.178578131960737	22.090339683198064
42	23.103252071349146	25.656739740872197	29.362186399150165	21.877821788628488
43	23.067472649940253	24.904830289266528	30.18320323180403	21.84449382898919
44	23.093329611395887	24.865104169892415	29.37884390047677	22.66272231823493
45	23.069512079551956	24.506068676535346	29.412931139501364	23.01148810441133
46	23.019049801497676	24.635172086008165	29.10241955907843	23.24335855341573
47	23.206527024735742	24.761305754267493	29.066621997888998	22.96554522310777
48	23.270163966253683	24.72978399849015	29.299047005898355	22.70100502935781
49	22.422634548647665	25.586359983789254	29.191312257897096	22.799693209665982
50	23.137854998420416	25.162174172938084	29.216713133442106	22.483257695199395
51	23.11132427368772	24.9770395727864	29.03173919991803	22.879896953607847
52	23.196340242266096	24.894589087455277	28.86609968104824	23.042970989230387
53	23.600538606363678	25.15757703427079	28.161395121192	23.08048923817353
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4875.0
1	3285.5
2	1696.0
3	1388.0
4	1080.0
5	1135.0
6	1190.0
7	1381.0
8	2072.0
9	2572.0
10	4040.0
11	5508.0
12	8426.5
13	11345.0
14	16105.5
15	20866.0
16	30919.5
17	40973.0
18	57344.0
19	73715.0
20	105718.0
21	137721.0
22	800641.0
23	1463561.0
24	1057857.5
25	601481.5
26	550809.0
27	575594.5
28	600380.0
29	646003.0
30	691626.0
31	747471.0
32	803316.0
33	889207.0
34	975098.0
35	1077179.5
36	1179261.0
37	1292415.5
38	1405570.0
39	1525235.5
40	1644901.0
41	1766234.5
42	2019705.0
43	2151842.0
44	2305934.0
45	2460026.0
46	2590022.5
47	2720019.0
48	2864120.0
49	3008221.0
50	2951147.0
51	2894073.0
52	2839799.0
53	2785525.0
54	2693250.5
55	2600976.0
56	2466181.0
57	2331386.0
58	2056209.5
59	1540565.0
60	1300097.0
61	1116463.5
62	932830.0
63	786864.0
64	640898.0
65	517287.5
66	393677.0
67	305414.5
68	217152.0
69	166742.0
70	116332.0
71	83514.0
72	50696.0
73	37228.5
74	23761.0
75	17813.0
76	8761.5
77	5658.0
78	4230.5
79	2803.0
80	2139.0
81	1475.0
82	1053.0
83	631.0
84	489.5
85	348.0
86	294.0
87	240.0
88	232.0
89	224.0
90	133.0
91	42.0
92	29.0
93	13.0
94	10.0
95	7.0
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	3.8589221E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.44453781017857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.62921763489318	33.61305622728234
2	12.780462090552206	11.36043461230203
3	4.174640732606293	5.56619933652696
4	1.986004933175168	3.5306828537481976
5	1.1509570215219282	2.557687643046093
6	0.7367672545476793	1.964716806122748
7	0.5035998658684429	1.5667584295853607
8	0.36963706743119357	1.3142678895591369
9	0.2895805244084756	1.1583245309547452
>10	2.120880077081107	19.210049644810436
>50	0.18617223551417886	5.623769650247094
>100	0.06612599760977447	4.939199941249258
>500	0.0038350770609273843	1.1871298485832045
>1k	0.0018736635822084468	1.5430671105939284
>5k	1.1991422122771877E-4	0.362586720774945
>10k+	1.2590992600302307E-4	4.502068754613735
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1075292	2.7865086988928853	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	159720	0.41389796388996813	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	71662	0.18570470753996304	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	53528	0.13871231036252327	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	49301	0.12775847431592363	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	46892	0.1215157984142774	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.612687050614471E-4	0.0	0.0	5.1827944388926636E-6	5.1827944388926636E-6
2	0.022594392356352567	2.5913972194463318E-6	0.0	7.774191658338995E-6	1.0365588877785327E-5
3	0.02267213427293596	2.5913972194463318E-6	0.0	1.0365588877785327E-5	2.3322574975016988E-5
4	0.024587176818106796	2.5913972194463318E-6	0.0	1.554838331667799E-5	2.3322574975016988E-5
5	0.02460013380420403	5.1827944388926636E-6	2.5913972194463318E-6	1.8139780536124324E-5	3.368816385280232E-5
6	0.024605316598642922	7.774191658338995E-6	2.5913972194463318E-6	2.0731177755570654E-5	4.146235551114131E-5
7	0.024605316598642922	1.2956986097231659E-5	2.5913972194463318E-6	2.5913972194463318E-5	4.6645149950033975E-5
8	0.024605316598642922	1.2956986097231659E-5	2.5913972194463318E-6	3.109676663335598E-5	4.6645149950033975E-5
9	0.024646778954154062	1.2956986097231659E-5	2.5913972194463318E-6	3.109676663335598E-5	5.1827944388926636E-5
10	0.024771166020687487	1.2956986097231659E-5	2.5913972194463318E-6	3.368816385280232E-5	5.441934160837297E-5
11	0.024848907937270875	1.554838331667799E-5	2.5913972194463318E-6	3.368816385280232E-5	7.774191658338996E-5
12	0.025294628259015645	1.8139780536124324E-5	2.5913972194463318E-6	3.368816385280232E-5	9.329029990006795E-5
13	0.02541124113389073	1.8139780536124324E-5	2.5913972194463318E-6	3.887095829169498E-5	9.588169711951428E-5
14	0.025621144308665883	1.8139780536124324E-5	2.5913972194463318E-6	3.887095829169498E-5	1.0883868321674595E-4
15	0.02581549910012436	1.8139780536124324E-5	2.5913972194463318E-6	4.4053752730587645E-5	1.1661287487508494E-4
16	0.026007262494363387	1.8139780536124324E-5	2.5913972194463318E-6	4.9236547169480306E-5	1.3475265541120927E-4
17	0.026336369941233072	1.8139780536124324E-5	2.5913972194463318E-6	5.441934160837297E-5	1.4252684706954826E-4
18	0.02666288599088331	2.0731177755570654E-5	2.5913972194463318E-6	6.219353326671196E-5	1.5289243594733358E-4
19	0.026940165493364067	2.0731177755570654E-5	2.5913972194463318E-6	6.478493048615829E-5	1.6844081926401156E-4
20	0.027336649267939356	2.0731177755570654E-5	2.5913972194463318E-6	6.737632770560464E-5	1.788064081417969E-4
21	0.02778496098690357	2.0731177755570654E-5	2.5913972194463318E-6	6.737632770560464E-5	1.8917199701958223E-4
22	0.028220315719770555	2.0731177755570654E-5	2.5913972194463318E-6	6.737632770560464E-5	1.9694618867792122E-4
23	0.02874636935531816	2.0731177755570654E-5	2.5913972194463318E-6	7.515051936394363E-5	2.1508596921404556E-4
24	0.0292465090186713	2.0731177755570654E-5	2.5913972194463318E-6	8.292471102228262E-5	2.2286016087238455E-4
25	0.029865852954118977	2.3322574975016988E-5	2.5913972194463318E-6	8.810750546117529E-5	2.2286016087238455E-4
26	0.030521476450638896	2.5913972194463318E-5	2.5913972194463318E-6	9.329029990006795E-5	2.2545155809183088E-4
27	0.031130454797208787	2.5913972194463318E-5	2.5913972194463318E-6	9.329029990006795E-5	2.4099994140850886E-4
28	0.03184568042977597	2.850536941390965E-5	2.5913972194463318E-6	9.847309433896061E-5	2.5913972194463317E-4
29	0.03259977702063486	2.850536941390965E-5	2.5913972194463318E-6	1.0365588877785327E-4	2.6950531082241855E-4
30	0.03332277684486038	2.850536941390965E-5	2.5913972194463318E-6	1.0624728599729961E-4	2.7727950248075754E-4
31	0.03407946483293871	2.850536941390965E-5	5.1827944388926636E-6	1.0883868321674595E-4	2.8764509135854286E-4
32	0.034768776493311435	2.850536941390965E-5	5.1827944388926636E-6	1.0883868321674595E-4	2.928278857974355E-4
33	0.035494367714756406	2.850536941390965E-5	5.1827944388926636E-6	1.0883868321674595E-4	3.0319347467522084E-4
34	0.03635730298883204	2.850536941390965E-5	5.1827944388926636E-6	1.1143008043619228E-4	3.0578487189466717E-4
35	0.03703884045754642	2.850536941390965E-5	5.1827944388926636E-6	1.140214776556386E-4	3.187418579918988E-4
36	0.03767891557074966	2.850536941390965E-5	5.1827944388926636E-6	1.1661287487508494E-4	3.3947303574746946E-4
37	0.038420055175511315	2.850536941390965E-5	5.1827944388926636E-6	1.2956986097231659E-4	3.4983862462525483E-4
38	0.03909381845256736	2.850536941390965E-5	5.1827944388926636E-6	1.4252684706954826E-4	3.4983862462525483E-4
39	0.03986087202952348	2.850536941390965E-5	5.1827944388926636E-6	1.451182442889946E-4	3.550214190641475E-4
40	0.040576097662090665	2.850536941390965E-5	5.1827944388926636E-6	1.5030103872788725E-4	3.653870079419328E-4
41	0.04125504373358561	2.850536941390965E-5	5.1827944388926636E-6	1.5289243594733358E-4	3.809353912586108E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	662045	0.0	45.032455	1
GTGGTAT	671475	0.0	44.398632	6
AGAGTAC	662335	0.0	44.345703	19
TCAACGC	675985	0.0	44.321777	12
AGTGGTA	671665	0.0	44.316795	5
GTATCAA	673220	0.0	44.312172	9
GGTATCA	672855	0.0	44.31211	8
CAACGCA	677730	0.0	44.25967	13
AACGCAG	680675	0.0	44.240456	14
TGGTATC	671810	0.0	44.229576	7
ACGCAGA	674495	0.0	44.224228	15
ATCAACG	678085	0.0	44.208427	11
TATCAAC	676155	0.0	44.186207	10
AGCAGTG	675880	0.0	44.13256	2
CGCAGAG	676455	0.0	44.05753	16
GCAGTGG	678555	0.0	43.90629	3
CAGAGTA	673590	0.0	43.886974	18
CAGTGGT	679490	0.0	43.807137	4
GTACATG	463415	0.0	43.78648	22
GAGTACA	468925	0.0	43.6784	20
>>END_MODULE