FastQCFastQC Report
Thu 26 May 2016
SRR805259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20043172
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1311710.6544423208063075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT828200.037.837781
AGCAGTG920950.034.5144652
TCAACGC931650.034.02978512
CAACGCA933400.033.9659813
AACGCAG940600.033.73346714
ACGCAGA940700.033.71489315
GTGGTAT944400.033.632576
GTATCAA945150.033.593459
GGTATCA946150.033.567888
AGTGGTA947400.033.4863855
ATCAACG949650.033.4268411
TGGTATC950950.033.3836177
AGAGTAC944500.033.2483319
TATCAAC955300.033.21930310
CGCAGAG957950.033.0979716
GCAGTGG973400.032.63543
CAGTGGT984950.032.2216844
CAGAGTA980300.032.13720318
GCAGAGT999250.031.63122217
GTACATG716550.031.44811622
Produced by FastQC (version 0.11.5)