FastQCFastQC Report
Thu 26 May 2016
SRR805258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20864480
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6034802.8923797765388835No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC791680.37943912333305213No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG622130.29817661403495316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG425180.20378173815019593No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA247780.11875685375336456No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG213540.10234618835456238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3254750.045.4418831
GTATCAA3332800.044.449619
GTGGTAT3331300.044.4428186
TCAACGC3330200.044.44126512
CAACGCA3333700.044.39883813
AGCAGTG3333950.044.389872
GGTATCA3337250.044.3748478
AGTGGTA3335150.044.37465
ACGCAGA3338800.044.3380615
TATCAAC3341200.044.3336410
AACGCAG3341450.044.3155514
TGGTATC3340150.044.3131037
ATCAACG3345150.044.2700511
AGAGTAC3319250.044.16166319
CGCAGAG3352550.044.14499716
GCAGTGG3360500.044.0237773
CAGAGTA3349400.043.87429418
CAGTGGT3372650.043.8442764
GCAGAGT3380000.043.62378717
GAGTACT926450.043.51476320
Produced by FastQC (version 0.11.5)