##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805258_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20864480
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.51330984524896	37.0	37.0	37.0	35.0	37.0
2	36.493200118095444	37.0	37.0	37.0	35.0	37.0
3	38.3452894105197	39.0	39.0	39.0	37.0	39.0
4	38.26204367422529	39.0	39.0	39.0	37.0	39.0
5	38.16712872786669	39.0	39.0	39.0	37.0	39.0
6	38.20385133010743	39.0	39.0	39.0	37.0	39.0
7	38.21224880754277	39.0	39.0	39.0	37.0	39.0
8	39.18333890899749	40.0	40.0	40.0	38.0	40.0
9	39.20129948122359	40.0	40.0	40.0	38.0	40.0
10	39.008102909825695	40.0	39.0	40.0	37.0	40.0
11	39.118711082183694	40.0	40.0	40.0	38.0	40.0
12	39.16202426324548	40.0	40.0	40.0	38.0	40.0
13	39.21013799529152	40.0	40.0	40.0	38.0	40.0
14	39.26242844298061	40.0	40.0	40.0	38.0	40.0
15	39.24705394047683	40.0	40.0	40.0	38.0	40.0
16	39.25002281389232	40.0	40.0	40.0	38.0	40.0
17	39.214153575837976	40.0	40.0	40.0	38.0	40.0
18	39.172045217518004	40.0	40.0	40.0	38.0	40.0
19	39.1589792796178	40.0	40.0	40.0	38.0	40.0
20	39.155621755251026	40.0	40.0	40.0	38.0	40.0
21	39.10137151752644	40.0	40.0	40.0	38.0	40.0
22	39.11031446745857	40.0	40.0	40.0	38.0	40.0
23	38.82153947761938	40.0	39.0	40.0	37.0	40.0
24	38.93031880018098	40.0	39.0	40.0	37.0	40.0
25	38.99715118708925	40.0	40.0	40.0	37.0	40.0
26	38.957562373948456	40.0	40.0	40.0	37.0	40.0
27	38.93075178485157	40.0	40.0	40.0	37.0	40.0
28	38.92110261075282	40.0	40.0	40.0	37.0	40.0
29	38.89786877027369	40.0	40.0	40.0	37.0	40.0
30	38.84839162059155	40.0	40.0	40.0	37.0	40.0
31	38.74677854420527	40.0	40.0	40.0	36.0	40.0
32	38.69840422574634	40.0	39.0	40.0	36.0	40.0
33	38.61089233951673	40.0	39.0	40.0	35.0	40.0
34	38.55510609418495	40.0	39.0	40.0	35.0	40.0
35	38.524369933973915	40.0	39.0	40.0	35.0	40.0
36	38.453511566068265	40.0	39.0	40.0	35.0	40.0
37	38.32034994401969	40.0	38.0	40.0	35.0	40.0
38	38.173635048656855	40.0	38.0	40.0	35.0	40.0
39	38.093951346978216	40.0	38.0	40.0	35.0	40.0
40	38.027961588307015	40.0	38.0	40.0	35.0	40.0
41	37.98178876252847	40.0	38.0	40.0	34.0	40.0
42	37.89824059837581	40.0	38.0	40.0	34.0	40.0
43	37.983932070197774	40.0	38.0	40.0	35.0	40.0
44	37.99715482964349	40.0	38.0	40.0	35.0	40.0
45	37.96406668174812	40.0	38.0	40.0	35.0	40.0
46	37.92638325997101	40.0	37.0	40.0	35.0	40.0
47	37.94916029539198	40.0	37.0	40.0	35.0	40.0
48	37.92656466875762	40.0	37.0	40.0	35.0	40.0
49	37.853377366701686	40.0	37.0	40.0	35.0	40.0
50	37.760259014363164	40.0	37.0	40.0	34.0	40.0
51	37.56451754369148	40.0	37.0	40.0	34.0	40.0
52	37.35542476975223	40.0	36.0	40.0	34.0	40.0
53	36.992891411623965	40.0	36.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	12.0
13	32.0
14	157.0
15	404.0
16	915.0
17	1759.0
18	2917.0
19	4380.0
20	6739.0
21	10099.0
22	14640.0
23	19782.0
24	26762.0
25	35129.0
26	45212.0
27	58392.0
28	74092.0
29	93374.0
30	116521.0
31	146274.0
32	186100.0
33	240656.0
34	331462.0
35	504711.0
36	838776.0
37	1678330.0
38	3103127.0
39	1.3323724E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.895357085343129	37.54107459184221	29.13619702000721	19.42737130280745
2	23.345388909764345	40.54742797328282	17.575511107873286	18.531672009079543
3	37.34370087344616	23.01708453793241	19.55646630062192	20.082748287999507
4	20.958586075473722	21.964415120817772	20.395102106546627	36.681896697161875
5	20.997944832557533	37.17768187848439	21.8762748939825	19.948098394975574
6	36.25722280162266	22.233810763556054	20.796123363726295	20.71284307109499
7	21.31485663673382	21.729743564181806	37.14554592302324	19.809853876061133
8	35.43856832281465	22.82690965698642	21.87375865585914	19.86076336433978
9	35.137247609334146	22.51005057398986	22.338356862955607	20.01434495372039
10	20.033832618881465	22.716415649946704	37.262433571313544	19.987318159858287
11	19.88584426738649	37.493831621971886	22.50850248843968	20.11182162220194
12	19.839348979701388	22.732088218829322	37.273629632753845	20.154933168715445
13	19.81718212004325	22.7532917187488	22.516597585945107	34.91292857526284
14	20.06225412758909	37.650667545992036	22.237506038971496	20.049572287447376
15	19.952421531713227	37.46359842181545	22.273883652983447	20.310096393487882
16	20.057835134161024	22.41421305491438	22.45102681686771	35.07692499405689
17	35.1049966258445	22.296251811691448	22.380097658796196	20.21865390366786
18	20.176012054937388	22.38588261006265	22.370013534964688	35.068091800035276
19	20.17547525747107	37.432071156338424	22.411941251351582	19.980512334838924
20	35.28079300322845	22.447355505624873	22.372294924196527	19.899556566950146
21	20.264775350260347	37.266124053894465	22.422792228706395	20.046308367138792
22	34.954108609464505	22.325047161491685	22.68947033427145	20.031373894772358
23	20.22374868676334	22.41879979755067	37.27971173976059	20.0777397759254
24	20.123866015352405	37.1487331579795	22.4017708564987	20.3256299701694
25	20.00401639532833	22.49527905799713	22.537355352254167	34.963349194420374
26	20.35200493853669	32.959163132750014	26.575337607263638	20.11349432144966
27	20.56173937716157	22.285074921589228	37.150909104851884	20.002276596397323
28	31.2162296879673	22.189793371318146	26.558370973060434	20.035605967654117
29	31.33479482833984	22.245903085051726	26.376252846943704	20.043049239664732
30	31.102361525425025	22.253705819651387	26.395198921803946	20.248733733119636
31	26.68054511782704	23.94673147857028	27.558875179252013	21.813848224350664
32	24.266787382192128	25.757406846468257	28.18306998305254	21.79273578828708
33	23.979893100618852	25.024203814329425	28.592133616557902	22.403769468493824
34	23.25178964441002	24.493430941005958	29.287775204558177	22.96700421002584
35	22.856284939763654	24.90467052138371	29.297480694462553	22.941563844390085
36	22.95803202380313	25.10732594342155	29.213721118379176	22.720920914396142
37	23.578675337223835	24.648646886958122	29.345945837135652	22.426731938682394
38	23.68039366425619	24.725638980698296	29.246700612715966	22.34726674232955
39	23.825309808823416	24.684353504137174	29.01393181138471	22.4764048756547
40	23.075360612869336	25.157938275959907	28.8442654693527	22.922435641818055
41	23.406861805326564	25.665940392475633	28.562187986472704	22.3650098157251
42	23.429704454652118	25.610650253445087	28.705359539274404	22.25428575262839
43	23.301659087597677	24.994574511322593	29.530426830671075	22.173339570408658
44	23.43235009930753	24.82369558215685	28.841754024063864	22.902200294471754
45	23.435633190954196	24.517816883047168	28.855135618045598	23.191414307953035
46	23.246522319271794	24.626388963444093	28.715405320429742	23.411683396854365
47	23.31333922532457	24.755675674639388	28.691791024746365	23.239194075289678
48	23.36075473723764	24.712564128125887	28.72779959050022	23.198881544136256
49	22.698399384983475	25.56696356678911	28.615503477680726	23.11913357054669
50	23.480570807420076	25.12185781768824	28.550982339363358	22.84658903552832
51	23.281543561114393	24.967796944855564	28.633213001234637	23.11744649279541
52	23.56688975713749	24.854225938053574	28.462266972385603	23.116617332423335
53	23.884300016103925	25.06235477711402	27.86253958881314	23.190805617968913
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3907.0
1	2497.5
2	1088.0
3	961.0
4	834.0
5	882.5
6	931.0
7	1070.5
8	1528.5
9	1847.0
10	2646.5
11	3446.0
12	4877.0
13	6308.0
14	8232.5
15	10157.0
16	14950.0
17	19743.0
18	27438.0
19	35133.0
20	49712.0
21	64291.0
22	418714.0
23	773137.0
24	504972.5
25	240439.0
26	244070.0
27	264861.0
28	285652.0
29	313031.0
30	340410.0
31	369013.0
32	397616.0
33	442846.0
34	488076.0
35	547224.5
36	606373.0
37	673065.0
38	739757.0
39	804964.5
40	870172.0
41	947679.5
42	1107308.5
43	1189430.0
44	1278607.5
45	1367785.0
46	1432193.5
47	1496602.0
48	1579853.0
49	1663104.0
50	1645075.0
51	1627046.0
52	1589754.5
53	1552463.0
54	1501120.5
55	1449778.0
56	1389614.0
57	1329450.0
58	1155226.5
59	843928.0
60	706853.0
61	610516.0
62	514179.0
63	438937.0
64	363695.0
65	296324.5
66	228954.0
67	179883.0
68	130812.0
69	99828.0
70	68844.0
71	46795.5
72	24747.0
73	16796.0
74	8845.0
75	6053.0
76	2030.0
77	799.0
78	539.0
79	279.0
80	222.5
81	166.0
82	128.0
83	90.0
84	72.5
85	55.0
86	43.5
87	32.0
88	26.0
89	20.0
90	17.0
91	14.0
92	9.5
93	6.0
94	7.0
95	5.5
96	4.0
97	4.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.086448E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.3752856360642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.92704141617659	32.06606537655807
2	13.036205920610474	11.30898310834061
3	4.498102522998268	5.853194452660528
4	2.1654803563933687	3.757133159913939
5	1.204448022771783	2.6121638510759424
6	0.8419128892104953	2.1910927230113586
7	0.5801130240067774	1.7613797682246457
8	0.4514116051343665	1.5664085849709894
9	0.3639196623134107	1.4206607371281796
>10	2.7419458162208707	22.308646447196217
>50	0.1341632218243121	3.890686033113191
>100	0.048189120909649344	3.8259396574404017
>500	0.004941805262757021	1.5057251603166864
>1k	0.0019367420847655097	1.4188627214342733
>5k	6.630849963727158E-5	0.20449906653715674
>10k+	1.2156558266832829E-4	4.308559152077792
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	603480	2.8923797765388835	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	79168	0.37943912333305213	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	62213	0.29817661403495316	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	42518	0.20378173815019593	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	24778	0.11875685375336456	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	21354	0.10234618835456238	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.75789667415627E-4	0.0	0.0	0.0	2.875700712406923E-5
2	0.0010592164290698833	0.0	0.0	4.792834520678206E-6	3.8342676165425645E-5
3	0.0010640092635905616	0.0	0.0	4.792834520678206E-6	6.230684876881667E-5
4	0.001073594932631918	0.0	0.0	4.792834520678206E-6	7.668535233085129E-5
5	0.001078387767152596	0.0	0.0	4.792834520678206E-6	1.2461369753763335E-4
6	0.0011454874504420911	0.0	0.0	4.792834520678206E-6	1.3899220109966795E-4
7	0.0011502802849627694	0.0	0.0	4.792834520678206E-6	1.7254204274441538E-4
8	0.0011550731194834474	0.0	0.0	4.792834520678206E-6	1.821277117857718E-4
9	0.0011790372920868384	0.0	0.0	4.792834520678206E-6	2.1088471890984102E-4
10	0.001188622961128195	0.0	0.0	4.792834520678206E-6	2.3005605699255385E-4
11	0.0011982086301695514	0.0	0.0	4.792834520678206E-6	2.6360589863730127E-4
12	0.0012030014646902294	0.0	0.0	4.792834520678206E-6	2.827772367200141E-4
13	0.0012269656372936207	0.0	0.0	4.792834520678206E-6	3.0674140932340516E-4
14	0.0012653083134590462	0.0	0.0	9.585669041356411E-6	3.4029125096815256E-4
15	0.0012748939825004027	0.0	0.0	9.585669041356411E-6	3.4508408548883075E-4
16	0.0012940653205831155	0.0	0.0	9.585669041356411E-6	3.8821959617493465E-4
17	0.0013132366586658282	0.0	0.0	9.585669041356411E-6	4.025980997369692E-4
18	0.0013419936657898975	0.0	0.0	9.585669041356411E-6	4.313551068610385E-4
19	0.001356372169351932	0.0	0.0	9.585669041356411E-6	4.601121139851077E-4
20	0.001380336341955323	0.0	0.0	9.585669041356411E-6	4.984547901505334E-4
21	0.001399507680038036	0.0	0.0	9.585669041356411E-6	5.128332937125679E-4
22	0.0014330575216827833	0.0	0.0	9.585669041356411E-6	5.511759698779936E-4
23	0.0014522288597654963	0.0	0.0	1.4378503562034615E-5	6.134828186468103E-4
24	0.0015001572049722783	0.0	0.0	1.4378503562034615E-5	6.470326602915577E-4
25	0.0015384998811377038	0.0	0.0	2.3964172603391025E-5	6.518254948122359E-4
26	0.0015720497227824514	0.0	0.0	2.3964172603391025E-5	6.662039983742705E-4
27	0.0016391494060719462	0.0	0.0	2.3964172603391025E-5	7.141323435810526E-4
28	0.0016822849167580502	4.792834520678206E-6	0.0	2.875700712406923E-5	7.620606887878347E-4
29	0.0017206275929234757	4.792834520678206E-6	0.0	2.875700712406923E-5	7.764391923498693E-4
30	0.0017445917655268667	4.792834520678206E-6	0.0	2.875700712406923E-5	8.051961994739384E-4
31	0.0018260699523783962	4.792834520678206E-6	0.0	2.875700712406923E-5	8.147818685152949E-4
32	0.0018979624701885693	4.792834520678206E-6	0.0	2.875700712406923E-5	8.38746041118686E-4
33	0.001979440657040099	4.792834520678206E-6	0.0	2.875700712406923E-5	8.675030482427551E-4
34	0.0020513331748502717	9.585669041356411E-6	0.0	2.875700712406923E-5	8.914672208461462E-4
35	0.0021328113617018014	9.585669041356411E-6	0.0	2.875700712406923E-5	9.154313934495372E-4
36	0.0022047038795119742	9.585669041356411E-6	0.0	2.875700712406923E-5	9.441884005736064E-4
37	0.0023005605699255387	9.585669041356411E-6	0.0	2.875700712406923E-5	9.681525731769974E-4
38	0.002410795763901137	9.585669041356411E-6	0.0	2.875700712406923E-5	9.82531076739032E-4
39	0.002540202295959449	9.585669041356411E-6	0.0	3.8342676165425645E-5	9.873239112597103E-4
40	0.0026600231589764037	1.4378503562034615E-5	0.0	3.8342676165425645E-5	0.0010017024148217448
41	0.002808601029117428	1.9171338082712823E-5	0.0	3.8342676165425645E-5	0.0010448379255078488
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	325475	0.0	45.441883	1
GTATCAA	333280	0.0	44.44961	9
GTGGTAT	333130	0.0	44.442818	6
TCAACGC	333020	0.0	44.441265	12
CAACGCA	333370	0.0	44.398838	13
AGCAGTG	333395	0.0	44.38987	2
GGTATCA	333725	0.0	44.374847	8
AGTGGTA	333515	0.0	44.3746	5
ACGCAGA	333880	0.0	44.33806	15
TATCAAC	334120	0.0	44.33364	10
AACGCAG	334145	0.0	44.31555	14
TGGTATC	334015	0.0	44.313103	7
ATCAACG	334515	0.0	44.27005	11
AGAGTAC	331925	0.0	44.161663	19
CGCAGAG	335255	0.0	44.144997	16
GCAGTGG	336050	0.0	44.023777	3
CAGAGTA	334940	0.0	43.874294	18
CAGTGGT	337265	0.0	43.844276	4
GCAGAGT	338000	0.0	43.623787	17
GAGTACT	92645	0.0	43.514763	20
>>END_MODULE