FastQCFastQC Report
Thu 26 May 2016
SRR805257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18533010
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT657990.35503676952637486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT469900.039.618431
TCAACGC572150.032.76815812
CAACGCA574200.032.69618613
AACGCAG582200.032.3599314
GTGGTAT577850.032.2822536
GGTATCA580000.032.1382758
ACGCAGA582950.032.08851615
AGTGGTA582000.032.05615
AGCAGTG584700.032.0447242
GTATCAA582300.032.0315139
ATCAACG587100.032.0017911
TGGTATC586800.031.7097847
GCAGTGG593700.031.5668683
TATCAAC594200.031.48098410
CGCAGAG599550.031.1804716
AGAGTAC595950.030.97843719
CAGTGGT604800.030.870954
CAGAGTA619900.029.9521718
GCAGAGT639850.029.17254317
GAGTACT193150.028.64043420
Produced by FastQC (version 0.11.5)